Influence of a temporary restriction of dietary protein in prepubertal ewe lambs on first lactation milk traits and response to a mammary gland inflammatory challenge

Pelayo, R.; Marina, H.; Suárez-Vega, Aroa; Hervás, Gonzalo; Esteban-Blanco, Cristina; Gausserès, Blandine; Foucras, Gilles; Arranz, Juan José; Gutiérrez-Gil, Beatriz

doi:10.1016/j.rvsc.2023.04.006

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…IL-6 has been recognized as an early but non-specific indicator of mastitis [24,27,31]. Increased concentrations of IL-6 in milk and blood were detected by the authors in cows and in ewes with natural infection of the udder but also in the case of experimentally induced mastitis and even after administration of bacterial lipopolysaccharides (LPS) [24,26,28,32]. Previous studies indicate that the level of IL-6 depends on a number of factors, including the form of mastitis course, the phase of infection, and the type of microorganisms causing mastitis [26,27,31].…”

Section: Discussionmentioning

confidence: 97%

Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by Streptococcus spp.

Bochniarz,

Ziomek,

Szczubiał

et al. 2024

Animals

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The aim of the study was to evaluate the concentrations of Interleukin-6 (IL-6) in milk and serum of healthy cows (HE) and cows with mastitis caused by Streptococcus spp. The blood and milk samples were obtained from Holstein-Friesian cows (Lublin region, Poland). A total of 43 milk and serum samples from 28 cows with mastitis and 15 healthy cows were selected for study. IL-6 levels in milk from HE cows ranged from 6.09–80.24 pg/mL (median 26.6 pg/mL) and were significantly lower than in milk from both cows with clinical and subclinical mastitis (487.09 pg/mL vs. 26.6 pg/mL in CM, p < 0.001; and 165.31 pg/mL vs. 26.6 pg/mL in SCM, p < 0.001). The IL-6 concentration in the serum of HE was not significantly different from the serum IL-6 of the entire group of mastitis cows, regardless of whether the inflammation proceeded in a clinical or subclinical form (44.37 pg/mL vs. 78.09 pg/mL; 128.29 pg/mL vs. 78.09 pg/mL, respectively). The present study indicates that cows with mastitis caused by Streptococcus spp. develop a local immune response in the mammary gland in response to the pathogen. Monitoring of IL-6 levels in milk can allow early detection of mastitis, which is especially important in cases of subclinical inflammation.

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Section: Discussionmentioning

confidence: 97%

Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by Streptococcus spp.

Bochniarz,

Ziomek,

Szczubiał

et al. 2024

Animals

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Milk transcriptome biomarker identification to enhance feed efficiency and reduce nutritional costs in dairy ewes

Suárez-Vega,

Gutiérrez-Gil,

Fonseca

et al. 2024

animal

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Epigenetic regulation of functional candidate genes for milk production traits in dairy sheep subjected to protein restriction in the prepubertal stage

Fonseca,

Suárez-Vega,

Esteban-Blanco

et al. 2023

BMC Genomics

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Background As the prepubertal stage is a crucial point for the proper development of the mammary gland and milk production, this study aims to evaluate how protein restriction at this stage can affect methylation marks in milk somatic cells. Here, 28 Assaf ewes were subjected to 42.3% nutritional protein restriction (14 animals, NPR) or fed standard diets (14 animals, C) during the prepubertal stage. During the second lactation, the milk somatic cells of these ewes were sampled, and the extracted DNA was subjected to whole-genome bisulfite sequencing. Results A total of 1154 differentially methylated regions (DMRs) were identified between the NPR and C groups. Indeed, the results of functional enrichment analyses of the genes harboring these DMRs suggested their relevant effects on the development of the mammary gland and lipid metabolism in sheep. The additional analysis of the correlations of the mean methylation levels within these DMRs with fat, protein, and dry extract percentages in the milk and milk somatic cell counts suggested associations between several DMRs and milk production traits. However, there were no phenotypic differences in these traits between the NPR and C groups. Conclusion In light of the above, the results obtained in the current study might suggest potential candidate genes for the regulation of milk production traits in the sheep mammary gland. Further studies focusing on elucidating the genetic mechanisms affected by the identified DMRs may help to better understand the biological mechanisms modified in the mammary gland of dairy sheep as a response to nutritional challenges and their potential effects on milk production.

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Influence of a temporary restriction of dietary protein in prepubertal ewe lambs on first lactation milk traits and response to a mammary gland inflammatory challenge

Cited by 4 publications

References 35 publications

Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by Streptococcus spp.

Interleukin-6 as a Milk Marker of Clinical and Subclinical Intramammary Infections (IMI) in Cows Caused by Streptococcus spp.

Milk transcriptome biomarker identification to enhance feed efficiency and reduce nutritional costs in dairy ewes

Epigenetic regulation of functional candidate genes for milk production traits in dairy sheep subjected to protein restriction in the prepubertal stage

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