Influence of acute exposure to a low dose of systemic insecticide fipronil on locomotor activity and emotional behavior in adult male mice

Maeda, Mizuki; Yokoyama, Toshifumi; Kitauchi, Sayaka; Hirano, Tetsushi; Mantani, Youhei; Tabuchi, Yoshiaki; Hoshi, Nobuhiko

doi:10.1292/jvms.20-0551

Cited by 5 publications

(4 citation statements)

References 24 publications

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“…The male pups were subjected to the open field test (OF) and the elevated plus maze test (EPM) at 3 and 10 weeks of age under conditions described in a previous study [ 22 ]. The OF was performed to assess locomotor activity and anxiety-like behavior in a novel environment.…”

Section: Methodsmentioning

confidence: 99%

Developmental stage-specific exposure and neurotoxicity evaluation of low-dose clothianidin during neuronal circuit formation

Shoda

Murata

KIMURA

et al. 2023

The Journal of Veterinary Medical Science

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Neonicotinoid pesticides (NN) were recently reported to exhibit adverse effects in higher vertebrates.Moreover, NNs are routinely transferred from mother to offspring, raising concerns about their effects on future generations. The fetal and neonatal periods are the most critical to the formation of neural circuits in the brain through neurogenesis and differentiation, neuronal migration, axon guidance, and synaptogenesis. NN exposure throughout the fetal and neonatal periods was found to affect the neurobehavior of the offspring, but the stage-specific neurobehavioral effects are unclear. We exposed fetal and neonatal mice to a no-observed-adverse-effect level (NOAEL) of clothianidin (CLO) for 4 days during each of four developmental stages: neurite proliferation and differentiation (fetal days 9-12, CLO-1), neurite outgrowth (fetal days 15-18, CLO-2), synapse formation and astrocyte differentiation (days 1-4 after birth, CLO-3), and synapse remodeling (days 11-14 after birth, CLO-4). CLO's neurobehavioral effects were evaluated in juveniles and adults, revealing that CLO-1 and CLO-2 caused behavioral abnormalities in adult mice. CLO-3 significantly increased locomotor activity and decreased juvenile neurons in the hippocampal dentate gyrus in adulthood. Comprehensive gene analysis of CLO-3 revealed high expression of genes related to neurite outgrowth and axonal branching in the hippocampus in juveniles and adults. These results revealed developmental stage-specific effects of a NOAEL of CLO in the fetal and neonatal periods, suggesting that the susceptibility of the fetus and neonate to CLO varies by developmental stage.

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Section: Methodsmentioning

confidence: 99%

Developmental stage-specific exposure and neurotoxicity evaluation of low-dose clothianidin during neuronal circuit formation

Shoda

Murata

KIMURA

et al. 2023

The Journal of Veterinary Medical Science

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“…Fipronil disrupts the central nervous system by blocking GABA‐gated chloride channels and glutamate‐gated chloride channels (Alengebawy et al, 2021). This causes hyperexcitation of the nerves and muscles resulting in excess neuronal stimulation and death of the target insect of target insects (Maeda et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Five‐day dietary LC 50 s for bobwhite quail and mallard ducks were 49 mg/kg and 5 g/kg, respectively (Koslowski et al, 2020). LC 50 s for midges ( Chironomus dilutus ), mysid shrimp ( Neomysis americana ), grass shrimp ( Palaemonetes pugio ), black flies ( Simulium vittatum ), and Daphnia were 0.082, 0.14, 0.32, 0.18–0.31, and 190 μg/L, respectively (CDC, 2005; Maeda et al, 2021). Some of the direct toxic effects of fipronil to these organisms are severe neurodegeneration in the notochord, altered body shape, and abnormal locomotion in response to a touch stimulus, convulsions, tremors, abnormal, hunched posture and in severe cases death (Gibbons et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Fipronil use and associated effects on hematological and biochemical parameters of blue land crab (Cardisoma guanhumi Latreille): Ecological implication

et al. 2021

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Fipronil is used to control pests to improve farm yield, however, indiscriminate use of fipronil has been reported to endanger crabs leading to their extinction. Therefore, this study investigated the impact of fipronil on several hematological and biochemical parameters of blue land crabs. We exposed blue land crabs to either fipronil or to a control treatment; fipronil reduced the protein content of the crab and also led to hematological and oxidative damages to the crabs' oxy‐hemocyanin. Based on our results, there is need for guided use of agrochemicals such as fipronil to avoid/reduce their adverse effects on economically important species such as crabs.

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“…Male offspring were subjected to the open field test (OF) and the elevated plus maze test (EPM) at 10 weeks of age under conditions described previously [ 23 ]. In the OF, locomotor activity and anxiety-like behavior were assessed in a novel environment.…”

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confidence: 99%

Transgenerational effects of developmental neurotoxicity induced by exposure to a no-observed-adverse-effect level (NOAEL) of neonicotinoid pesticide clothianidin

et al. 2023

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Neonicotinoid pesticides (NNs) transfer rapidly from mother to offspring, which exhibit neurobehavioral effects. However, no studies have investigated NNs’ transgenerational effects. We exposed F0 generation mice (mothers) to a no-observed-adverse-effect level (NOAEL) of clothianidin (CLO) during gestation and lactation, and examined the adult neurobehavioral effects of three generations of offspring (F1, F2, F3). F1 had lower birth weight, decreased locomotor activity, and increased anxiety-like behavior. In F2, body weight was affected, and there was a decreasing trend in locomotor activity and an increasing trend in anxiety-like behavior. In F3, locomotor activity tended to increase. Thus, even when only the mothers were exposed, the effects of CLOs were still observed in F1, F2, and F3 but the effects became smaller.

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Influence of acute exposure to a low dose of systemic insecticide fipronil on locomotor activity and emotional behavior in adult male mice

Cited by 5 publications

References 24 publications

Developmental stage-specific exposure and neurotoxicity evaluation of low-dose clothianidin during neuronal circuit formation

Developmental stage-specific exposure and neurotoxicity evaluation of low-dose clothianidin during neuronal circuit formation

Fipronil use and associated effects on hematological and biochemical parameters of blue land crab (Cardisoma guanhumi Latreille): Ecological implication

Transgenerational effects of developmental neurotoxicity induced by exposure to a no-observed-adverse-effect level (NOAEL) of neonicotinoid pesticide clothianidin

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