Influence of Additives on Enhanced In vitro Shoot Multiplication of Orthosiphon aristatus (Blume) Miq.

Abstract

Orthosiphon aristatus is a valuable medicinal plant and different parts of the plant are pharmaceutically used for the treatment of various diseases. The present study was designed to develop an efficient protocol for micropropagation of O. aristatus from nodal explants and to study the influence of additives on the enhancement of the number of shoots per explant. Among the different types of additives used, 10% coconut water and 30 mg/L glutamine added to Murashige and Skoog's (MS) medium supplemented with 1.0 mg/L 6-benzyl amino purine (BAP) and 0.5 mg/L kinetin (KIN) was found to be most effective. Maximum number of shoots (44.07 ± 0.38) with 100% shooting response and shoot length of 7.47 ± 0.10 cm was recorded. In vitro rooting of the microshoots was achieved on halfstrength MS medium containing 0.5 mg/L indole-3-butyric acid (IBA), producing an average of 30.27 ± 0.36 roots and 6.02 ± 0.20 cm root length. The rooted shoots were acclimatized with 100% survival rate on coco pith: soil (3:1) planting substrate and was successfully transferred to field conditions. The hardened plants exhibited homogeneity and no morphological variations were observed among the regenerants and the mother plants. Thus, the procedure described is a quick and reliable method which could be applied for efficient large-scale propagation, genetic transformation assays and secondary metabolite production.

RETRACTED: Review on Medicinal Plants Propagation: A Comprehensive Study on Role of Natural Organic Extracts in Tissue Culture Medium

“…Amino acids glutamine and asparagine significantly improved shoot multiplication in Orthosiphon arisstatus [49] is well documented. In Syzyzium cumini [60] in vitro studies lower levels of L-glutamine (50 & 100 mg/L) had no significant effect on axillary shoot development.…”

“…It was also observed that cultures grown on without CW turned brown after four weeks and need to transfer to fresh medium, whereas addition of coconut water to the medium had made the cultures survival for 8 weeks. In Orthosiphon aristatus [49], supplementation of CW reduces the time required for bud break and shoot initiation.…”

Influence of Additives on Enhanced in Vitro Shoot Multiplication of <i>Stevia rebaudiana</i> (Bert.)—An Important Anti Diabetic Medicinal Plant

“…Both of them are used in tissue culture media, but the coconut water is the more complex combination of compounds [33]. CW contains growth hormones and is liberally made use in tissue culture [12]. When added to the culture medium containing auxin, the liquid CW can induce plant cells to divide and grow rapidly this is clearly evident in the present research addition of CW into the media enhanced shoot growth and maximum shoot length (8.0 cm) was obtained on 15% CW supplemented medium.…”

“…Minor quantities of complex organic nutrients like amino acids, peptides, fatty acids, carbohydrates, vitamins on high proliferation and regeneration of plants in vitro, are well documented in many medicinal crops [12][13][14].…”

Induction Of Callus From Different Explants Of Bacopa monnieri And Effect Of Adjuvant On The Growth Rate Of The Calli

“…Tissue culture studies for micropropagation and callus regeneration have been done by Arumugam and Bhojwani (1990), Rani and Grover (1999), Martin (2004), Tsay Hsin-Sheng and Agarwal, (2005), Wood Ward and Bartel (2005), Sakakibara (2006), Bahadur et al, (2007), Chaturvedi et al, (2007), Monga et al, (2008, Wang et al, (2008), Siva et al, (2009), Dash and Mandal (2010), Joshi et al, (2010), Juan et al, (2010), Showkat et al, (2010), Ahmad et al, (2011), Madhulkar et al, (2011), Rao et al, (2011), Rout et al, (2011), Subashri and Kolipillai (2012), Jayakumar and Lingam (2013), Kalsaitkar et al, (2014), Kaur et al, (2013), Osman et al, (2013), Sundriyal et al, (2013), Kumari Ritika et al, (2014, Subhashri and Pillai (2014), Thangavel et al, (2014), Anita (2017), Aradkani et al, (2017), Abiri et al, (2017), Khattak et al, (2017), Rajeev et al, (2018) and Srivastva et al, (2017). In the present work impact of plant growth regulators & adjuvant on the growth of callus have been studied.…”