Influence of agar content on the growth parameters of Bacillus cereus

Stecchini, Mara Lucia; Torre, Manuela Del; Donda, Stefania; Maltini, Enrico; Pacor, Sabrina

doi:10.1016/s0168-1605(00)00436-0

Cited by 22 publications

(22 citation statements)

References 13 publications

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“…However, the high The variations in the findings of short and extreme drying conditions must be related to loss of agar media water content, which was found to be reduced by 2.49% -7.84% in case of short drying and 15.51% -61.57% in case of extreme drying conditions. Finding of this study is in perfect analogy with the results of previous investigation [25,26], where 1% and 7% agar was used in media for the growth of Bacillus cereus to mimic short and extreme drying conditions; and observe its effect on available surface water layer. In their study it was observed that Bacillus colony under extreme air-drying condition (7% agar) grew more vertically compared to less drying condition (1% agar), where colony grew more laterally.…”

Section: Extreme Air-drying Conditionssupporting

confidence: 62%

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Effects of Preparation and Storage of Agar Media on the Sensitivity of Bacterial Forward Scattering Patterns

Mialon¹,

Tang²,

Singh³

et al. 2012

OJAB

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Recent worldwide foodborne outbreaks emphasize the need for the development of rapid and accurate method for pathogen detection. To address such issues, a new colony based label-free detection method working on the principles of elastic light scattering was introduced. In order to build libraries of scattering images for bacterial pathogens, it is pertinent to determine the effect of preparation and storage of the agar media on the scatter patterns. Scatter patterns of three Escherichia coli serovars (O26, O111 and O157) were studied and used in a model system, after growth on Sorbitol-MacConkey agar plates that were prepared and stored at different conditions in the laboratory. Quantitative image processing software was used to analyze variation in scatter patterns of the same serovar on media prepared under various standard laboratory conditions and to generate a cross-validation matrix for comparison. Based on the results, it was determined that attention should be given during preparation of media so that the agar plates are not air-dried more than 10 -20 min after solidification at room temperature. The plates could be stored in sealed bags in cold room (4˚C -10˚C) for up to a month before use. The findings of this study should provide guidelines in preparation, storage, and handling of media for generation of reproducible scatter patterns of bacterial colonies with the light scattering sensor for pathogen detection.

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Section: Extreme Air-drying Conditionssupporting

confidence: 62%

“…Surface water layer (content) on SMAC agar plate was measured by following the published procedure [25,26] where they monitored the influence of agar on the growth of Bacillus cereus. Briefly, an 85 mm diameter filter paper (Whatman, Maidstone, UK) was placed for 1 min on the petri dish after agar solidification.…”

Section: Surface Water Layer Measurementmentioning

confidence: 99%

Effects of Preparation and Storage of Agar Media on the Sensitivity of Bacterial Forward Scattering Patterns

Mialon¹,

Tang²,

Singh³

et al. 2012

OJAB

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“…As well as measurement of growth rate, FCM has applications including the study during growth and/or differing growth conditions of cell physiology (Cronin and Wilkinson 2008c), changes in cell size (reflected by changes in FSC properties of cells; Robertson and Button 1999), changes in cytoplasmic density (reflected by changes in SSC properties of cells; Davey 1994), DNA (Reardon and Scheper 1993; Stecchini et al. 2001) and lipid or protein contents (Reardon and Scheper 1993) of B. cereus.…”

Section: Common Themes In Bacillus Cereus Research and The Utility Ofmentioning

confidence: 99%

“…As well as measurement of growth rate, FCM has applications including the study during growth and ⁄ or differing growth conditions of cell physiology (Cronin and Wilkinson 2008c), changes in cell size (reflected by changes in FSC properties of cells; Robertson and Button 1999), changes in cytoplasmic density (reflected by changes in SSC properties of cells; Davey 1994), DNA (Reardon and Scheper 1993;Stecchini et al 2001) and lipid or protein contents (Reardon and Scheper 1993) of B. cereus. The physiological status of cells may be assessed by staining with a number of fluorescent dyes to detect alterations in membrane permeability, enzyme activity, redox activity, membrane potential, oxidative damage to DNA and intracellular Ca 2+ and pH (Coder 1997;Nebe-von-Caron et al 2000;Sincock and Robinson 2001;Shapiro and Nebe-von-Caron 2004).…”

Section: Growthmentioning

confidence: 99%

The potential of flow cytometry in the study of Bacillus cereus

Cronin

Wilkinson

2010

Journal of Applied Microbiology

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Summary Flow cytometry (FCM) is a rapid method allowing the acquisition of multiparametric data from thousands of individual cells within a sample. As well as measuring the intrinsic light scattering properties of cells, a plethora of fluorescent dyes may be employed to yield information on macromolecule content, surface antigens present or physiological status. Despite FCM’s indispensability within other fields e.g. immunology, it is underutilized within microbiological research. In this review, a strong case is presented for the potential of FCM in the study of Gram‐positive spore‐former, Bacillus cereus. Previous reports where FCM was successfully used in the study of B. cereus are reviewed along with relevant studies involving other members of the genus. Under headings reflecting common research themes associated with B. cereus, specific instances where FCM has generated novel data, providing a unique insight into the organism, are discussed. Further applications are posited, based on the authors’ own research with FCM and B. cereus and work extant in the broader field of microbial cytometry. The authors conclude that, while the expense of equipment and reagents is an undeniable disadvantage, FCM is a technique capable of generating significantly novel data and allows the design and execution of experiments that are not possible with any other technique.

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“…2004). The surface water of agar media was found to be changed according to the different cross‐linking densities (Stecchini et al. 2001), whereas viscosity of the liquid phase of the media was changed by adding PVP (Stecchini et al.…”

Section: Introductionmentioning

confidence: 99%

Bacillus cereuscell and spore properties as influenced by the micro-structure of the medium

Stecchini

Spaziani

Torre

et al. 2009

Journal of Applied Microbiology

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Aim: To investigate the effect of different growth conditions on Bacillus cereus cell and spore properties. Methods and Results: Bacillus cereus was grown on agar plates with different surface water conditions (wet and dry) or viscosity. Cell populations displayed different types of behaviour, and heterogeneity was manifested in cell motility and dimension. Spore populations were heterogeneous regarding their properties, namely size and thermal resistance. The smallest spores were produced from flagellated cells, which also displayed jet‐motility, growing on the wettest agar. Cytometric analysis also revealed within the smallest spores a sub‐population labelled by propidium iodide (PI), indicating that spore populations were partly damaged. Nonmotile cells grown on diffusion‐limiting media were elongated and produced the least thermal‐resistant spores. Conclusions: The micro‐structural properties of the media were found to influence cell and spore properties. Abundant surface water enabled flagellar motility and resulted in a heterogeneous cell and spore population, the latter including small and damaged spores. High viscosity gave rise to filamentous cells and more heat‐sensitive spores. Significance and Impact of the Study: This study provides useful information on conditions resulting in heterogeneous populations of damaged and heat‐sensitive spores.

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Influence of agar content on the growth parameters of Bacillus cereus

Cited by 22 publications

References 13 publications

Effects of Preparation and Storage of Agar Media on the Sensitivity of Bacterial Forward Scattering Patterns

Effects of Preparation and Storage of Agar Media on the Sensitivity of Bacterial Forward Scattering Patterns

The potential of flow cytometry in the study of Bacillus cereus

Bacillus cereuscell and spore properties as influenced by the micro-structure of the medium

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