Influence of Age and Storage Temperature on RNA Metabolism in Durum Wheat Seeds

Grilli, Isa; Anguillesi, M.C.; Meletti, Paolo; Floris, C.; Galleschi, Luciano

doi:10.1016/s0044-328x(82)80186-4

Cited by 6 publications

(3 citation statements)

References 21 publications

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“…In Triticum durum a progressive rRNA deterioration has been recorded in embryos of ageing seeds. Embryos of cold stored old seeds maintain the structural stability of RNA and the whole amount of poly(A) + RNA, and this state persists during imbibition (Grilli et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Spanò

Buselli

Castiglione

et al. 2007

Journal of Plant Physiology

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Section: Introductionmentioning

confidence: 99%

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Spanò

Buselli

Castiglione

et al. 2007

Journal of Plant Physiology

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“…1978;BROCKLEHURST and FRASER 1980) and bring about a loss in the regulatory control of the embryo (BERJAK and VILUERS 1972;GRILLI et al 1982). As the histonic proteins play an important role in regulating the transcriptional activity of DNA (HUANG and BoNNER 1962;GEORGIEV 1969) our results are of particular interest because they show specific changes in the histone/DNA ratio for each area of an embryo subjected to storage.…”

Section: Resultsmentioning

confidence: 77%

Variation of Histone/DNA Ratio in the Embryonic Areas ofTriticum DurumAged Seeds

Innocenti

Bitonti

1984

Caryologia

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SUMMARY -The histone/DNA ratio has been estimated independently for each embryonic area in young and old caryopses of Triticum durum. As the ratio is already quite different in the single embryo areas of over dormant young seeds, it can be suggested that nuclear conditions at ripening time influence the response of the cell populations which undergo senescence. Furthermore, when old seeds are considered, it can be clearly observed that the single areas of the embryo exibit specific increases in the histone/DNA ratio. These observations are discussed in terms of a heterogeneity in the ageing process and loss of metabolic activity and regulatory control in the embryos of aged seeds.

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“…Progressive deterioration of 18S rRNA has been observed in dry non-viable rye embryos and wheat embryos 42 and lesions have been found to become amplified with progressive imbibition, either as a result of the intrinsic properties of the rRNA or due to an increase in the activity of other components such as nucleases 43 , 44 . Progressive rRNA deterioration has also been recorded in embryos of aging seeds of Triticum durum 45 . PCD, as revealed by DNA fragmentation and TUNEL assay, has been detected in aged seeds of Phaseolus vulgaris and Helianthus annuus , coincident with a loss of viability 46 , 47 .…”

Section: Discussionmentioning

confidence: 94%

Deterioration of willow seeds during storage

López-Fernández

Moyano

Correa

et al. 2018

Sci Rep

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Willow (Salix spp.) seeds are able to tolerate desiccation, but differ from typical orthodox seeds in that they lose viability in a few days at room temperature, and in that the chloroplasts in embryo tissues do not dedifferentiate during maturation drying, thus retaining chlorophyll and maintaining intact their thylakoid membranes. In the present study, we investigated the damage generated in willow seeds during storage under appropriate conditions to exclude the eventual generation of reactive oxygen species by photooxidation. To this end, we measured different indicators of molecular damage, such as changes in the fatty acid profile, protein degradation, nuclease activities, and DNA damage, and evaluated normal germination and total germination in seeds stored for one, ten and sixteen years. We found: (i) a decrease in the fraction of unsaturated fatty acids; (ii) changes in the protein profile due to a decrease in protein solubility; (iii) activation of nucleases; and (iv) DNA fragmentation. Taken together, our findings identified programmed cell death as a key mechanism in seed deterioration during storage. We also found that, although the seeds maintained high percentages of total germination, the death program had already started in the seeds stored for ten years and was more advanced in those stored for sixteen years.

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Influence of Age and Storage Temperature on RNA Metabolism in Durum Wheat Seeds

Cited by 6 publications

References 21 publications

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

RNases and nucleases in embryos and endosperms from naturally aged wheat seeds stored in different conditions

Variation of Histone/DNA Ratio in the Embryonic Areas ofTriticum DurumAged Seeds

Deterioration of willow seeds during storage

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