Influence of age on the response to fibroblast growth factor-2 treatment in a rat model of stroke

Won, Seok Joon; Xie, Lin; Kim, Sun Hee; Tang, Huidong; Wang, Yaoming; Mao, XiaoOu; Banwait, Surita; Jin, Kunlin

doi:10.1016/j.brainres.2006.09.055

Cited by 50 publications

(29 citation statements)

References 25 publications

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“…The few studies available in literature have shown varying results in the histological outcomes in young vs. aged animal 4, 6, 19–23 . There have been reports of increased 22, 24 , equal 23, 25 or decreased infarct volumes 4, 19–21 in aged vs. young animals. The discrepancies may be due to the difference in animal strains, animal sexes, or ischemic models used in the studies.…”

Section: Discussionmentioning

confidence: 99%

Perfusion of Ischemic Brain in Young and Aged Animals

Manwani

Friedler

Verma

et al. 2014

Stroke

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Background and Purpose Aging is an important determinant of ischemic stroke outcomes. Both clinical and experimental stroke studies have shown that aging negatively correlates with infarct volumes but is associated with worsened functional recovery after stroke. This may correspond to a differing cellular and molecular response to stroke in the aged vs. young brain. It was hypothesized in this study that the smaller injury seen in the aged ischemic brain is due to structural differences in microvasculature with aging or differences in intra-ischemic tissue perfusion. Methods Both young and aged C57BL6 mice were subject to middle cerebral artery occlusion (MCAO) modeling. Laser Speckle Flowmetry (LSF) was utilized to study the functional dynamics of cerebral perfusion, and FITC-dextran staining was performed to examine the structural change in microvasculature. In separate cohorts, Cresyl violet (CV) staining and immunohistochemistry with CD31 and IgG antibodies were applied to further assess the microvascular density and blood brain barrier breakdown after stroke. Results No difference in cerebral blood flow was seen at the baseline, intra-ischemically and post-reperfusion in young vs. aged mice. FITC-dextran and CD31 staining did not show significant differences in the microvascular density between young and aged ischemic brains. More extravasation of IgG through the BBB was found in the young vs. aged cohort at both 24 and 72 hours after stroke. Conclusions Cerebrovascular dynamics and perfusion are not responsible for the different stroke phenotypes seen in the young vs. aged animals, which may be more related to different levels of BBB breakdown.

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Section: Discussionmentioning

confidence: 99%

Perfusion of Ischemic Brain in Young and Aged Animals

Manwani

Friedler

Verma

et al. 2014

Stroke

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“…Male Sprague-Dawley rats weighing 280-310 g were anesthetized with 4% isoflurane in 70% N 2 O/30% O 2 using a mask. Permanent distal middle cerebral arterial (MCA) occlusion (MCAO) was performed as described previously (Nawashiro et al, 1997; Won et al, 2006). A 2-cm incision was made between the right orbit and tragus under the surgical microscope.…”

Section: Methodsmentioning

confidence: 99%

Transplantation of Human Neural Precursor Cells in Matrigel Scaffolding Improves Outcome from Focal Cerebral Ischemia after Delayed Postischemic Treatment in Rats

Jin

Mao

Xie

et al. 2009

J Cereb Blood Flow Metab

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164

136

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Transplantation of neural cells is a potential approach for the treatment of stroke, but the disruption of tissue architecture that accompanies stroke may limit the efficacy of transplantation. One strategy for enhancing the ability of transplants to restore brain structure and, thereby, function is to administer cells together with biomaterial scaffolding. We occluded the middle cerebral artery in adult rats and, 3 wks later, injected one of the following into the infarct cavity: (a) artificial cerebrospinal fluid, (b) Matrigel scaffolding, (c) human neuronal precursor cells, (d) scaffolding plus cells, or (e) cells cultured in and administered together with scaffolding. When tested up to 9 wks later, the latter group showed reduced infarct size, survival and neuronal differentiation of transplanted cells, and improved outcome on behavioral tests of sensorimotor and cognitive function. These results indicate that transplantation of human neural cells together with the scaffolding in which they are cultured has the potential to improve outcome from stroke, even when treatment is delayed for several weeks after the ischemic event.

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“…Focal ischemia was performed as previously described [28]. Briefly, immediately thereafter, both common carotid arteries (CCAs) were occluded with microclips and rats underwent simultaneous occlusion of the middle cerebral artery (MCA) and both CCAs for 60 min.…”

Section: Transient Focal Cerebral Ischemiamentioning

confidence: 99%

Effect of neural precursor proliferation level on neurogenesis in rat brain during aging and after focal ischemia

Tang

Wang

Xie

et al. 2009

Neurobiology of Aging

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The observed age-related decline in neurogenesis may result from reduced proliferation or increased death rate of neuronal precursor cells (NPCs). We found that caspase-3, but not caspase-6, -7, or -9, was activated in NPCs in neurogenic regions of young, young-adult, middle-aged and aged rat brains. The number of capase-3-immunoreactive cells was highest in young and lowest in aged rats. Surprisingly, intraventricular administration of a caspase-3 inhibitor failed to restore the number of BrdU-positive cells in the aged dentate gyrus, suggesting that the age-related decline in neurogenesis may be attributable primarily to reduced proliferation. Additionally, we also found that NPCs in the subventricular zone of young-adult and aged rat brain were increased after focal cerebral ischemia, suggesting that the increase in neurogenesis induced by ischemia may result from an increase in the rate of NPC proliferation, but not from a decrease in NPC death. Thus, our results suggest that agerelated and injury-induced changes in the rate of neurogenesis are controlled at the level of NPC proliferation. Furthermore, our results may imply that the mechanisms that maintain a stable population of NPCs in the normal adult and in the ischemic brain, which account for the observed age-dependent reduction or injury-induced increases in neurogenesis, impinge on the regulation of cell division at the NPC level.

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Influence of age on the response to fibroblast growth factor-2 treatment in a rat model of stroke

Cited by 50 publications

References 25 publications

Perfusion of Ischemic Brain in Young and Aged Animals

Perfusion of Ischemic Brain in Young and Aged Animals

Transplantation of Human Neural Precursor Cells in Matrigel Scaffolding Improves Outcome from Focal Cerebral Ischemia after Delayed Postischemic Treatment in Rats

Effect of neural precursor proliferation level on neurogenesis in rat brain during aging and after focal ischemia

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