An increasing number of nature reserves are being invaded by various development and construction activities, such as energy, resources, and transportation facilities. The ecological footprint model, which enables a quantitative assessment of ecological sustainability, can assess whether human consumption at various spatial scales falls within the regenerative capacity of the biosphere. Based on the traditional ecological footprint evaluation model: the Global Agro-Ecological Zone (EF-GAEZ model), this study proposes an improved ecological footprint model based on net primary productivity (EF-NPP model) and its validations. In this study, the status of ecological footprints and the ecological carrying capacities of 319 national nature reserves in 2010 is explored, and the changes in ecological surpluses and ecological deficits from 2000 to 2010 are analyzed. The ecological footprint per capita and the ecological carrying capacity per capita calculated by the two models were mostly consistently at the same level (more than 68%), which indicated that the ecological footprint per capita and the ecological carrying capacity per capita of the two models followed the same rule. The EF-NPP model can reflect the change in the global climate, the degradation of the soil, and the progress of the technology.Sustainability 2019, 11, 2 2 of 16 of nature reserves have been developed for tourism, planting, or breeding industries, and local communities have continuously eroded the land of nature reserves [9][10][11]. This pressure greatly exceeds the ecological carrying capacity, which is an ecological concept that assumes that a limited number of individuals can be supported at a given level of consumption wherein the environment is not degraded [12], causing great damage to the main targets of protection and the resources and environment in these areas [13].One way to estimate human demand compared to an ecosystem's carrying capacity is "ecological footprint" accounting [14][15][16]. The ecological footprint is an accounting system of indicators based on the context that the Earth has a finite amount of biological production that supports all life on it [17]. Accounting for the ecological footprint is a non-monetized ecosystem assessment tool and a significant international biophysical method for distinguishing the level of development in recent years [18][19][20]. Bioproductive space has been used as a synonym for the renewable capacity of the Earth's ecosystem to measure the flow of energy into and out of the socioeconomic system, thereby characterizing the interdependence and supportive relation between human society and nature [21]. The ecological footprint could be used to express the space equivalents-individuals or countries occupying bioproductive areas [22]. The idea of this concept is to compare the area needed to support a certain lifestyle with the available area, thus providing a tool to assess whether the consumption is ecologically sustainable [23].The ecological footprint model can assess whether human consumpt...