Objectives: to verify the effectiveness of a regional nutrition training program for nurses based on four levels of assessment: response, learning, behavior, and conclusions. Methods: this was a longitudinal, almost experimental before and after type of study, carried out in 2014 with eight nurses and 62 mothers attended in the Primary Health Care Units of the rural area of Ceará, Brazil, in a training program using educational technology on regional food. Results: the evaluations showed a positive reaction from the nurses; a very good post-test average (92.3%); a change in nurses’ behavior, verified by 30 educational activities after training; and satisfactory results, verified by improvement in knowledge, attitude and practice of the mothers about regional food and food safety after participating in the educational interventions carried out by the trained nurses. Conclusions: the training program was considered effective, showing positive results in the four levels of evaluation.