Influence of an enzyme cocktail and phytase individually or in combination in Ven Cobb broiler chickens

Abstract

1. An experiment (21 d) was conducted to determine if the response of chicks to a cocktail of xylanase, amylase, and protease (XAP) or Escherichia coli-derived phytase individually or in combination when fed a nutritionally marginal maize-soybean meal diet is age-dependent. 2. A total of 250 one-day-old Ven Cobb broiler chicks were allocated to 5 dietary treatments in a randomised complete block design. The treatments were as follows: (1) positive control with supplemental inorganic P; (2) negative control (NC) marginal in P and ME; (3) NC plus XAP to provide (per kg of diet) 650, 1650 and 4000 U of xylanase, amylase, and protease, respectively; (4) NC plus phytase added to provide 1000 phytase units/kg; and (5) NC plus a combination of XAP and phytase. 3. Low ME and P in the NC diet depressed weight gain and gain:feed. A cocktail of XAP alone did not improve performance, but phytase supplementation improved weight gain. The enzymes were additive in their effects on growth performance. 4. The enzymes had no effect on ileal digestible energy. Ileal N digestibility was higher in diets with XAP or phytase individually compared with NC. Both phytase and XAP individually and in combination improved ileal P digestibility compared with NC. 5. Total tract nutrient retention and ME increased as the birds grew older. There were age x diet interactions in total tract retention of P and Ca; improvement in P retention due to phytase use decreased by 50% as the chicks matured. 6. The current study shows that a combination of XAP and phytase improved performance, but the enhancement in performance appears to be due mainly to phytase. Both XAP and phytase were effective in improving P digestibility and retention of chicks receiving nutritionally marginal maize-soybean meal. 7. The data also showed that the chicks benefited more from the enzyme addition at a younger age and that the contribution of the enzymes to nutrient retention decreased with age in chickens.

Enzyme Complex Added to Broiler Diets: Effects on Performance, Metabolizable Energy Content, and Nitrogen and Phosphorus Balance

“…These results are consistent with other studies that reported higher weight gain in 42-d-old broilers fed diets with multiple enzyme complexes (Figueiredo et al, 1998;West et al, 2007;García et al, 2008;Farran et al, 2010;Tiwari et al, 2010;Iwahashi et al, 2011).…”

The Effect of Olive Cake, with or Without Enzymes Supplementation, on Growth Performance, Carcass Characteristics, Lymphoid Organs and Lipid Metabolism of Broiler Chickens

“…This phytic acid acts as an anti-nutritional component and, in turn, reduces the utilization of feed (Ceylan et al, 2012). Therefore, many researchers have used phytase enzyme in poultry diets, and some of them have concluded that this enzyme increases the utilization of phytic acid in poultry diets (Tiwari et al,2010).…”

A review on role of exogenous enzyme supplementation in poultry<br>production

“…Ileal N digestibility was higher in diets with XAP or phytase individually compared with negative control diet. Both phytase and XAP individually and in combination improved ileal P digestibility compared with negative control (Tiwari et al, 2010). This study shows that a combination of XAP and phytase improved performance, but the enhancement in performance appears to be due mainly to phytase.…”

“…It was found that a cocktail of XAP alone did not improve performance, but phytase supplementation improved weight gain (Tiwari et al, 2010). The enzymes were additive in their effects on growth performance of the chicks.…”

“…In another study, Maize-soyabean based diet supplemented with mixture of xylanase, amylase, and protease (XAP) at 650, 1650 and 4000U, respectively, or Escherichia coli-derived phytase (1000 phytase units/kg) individually or in combination was fed to the chicks for 21 days (Tiwari et al, 2010). It was found that a cocktail of XAP alone did not improve performance, but phytase supplementation improved weight gain (Tiwari et al, 2010).…”