Influence of ancient anthropogenic activities on the mangrove soil microbiome

Huergo, Luciano F.; Rissi, Daniel Vasconcelos; Elias, Andressa S.; Gonçalves, Maria V.; Gernet, Marcos V.; Barreto, Flávio Ataliba Flexa Daltro; Dahmer, Gilson W.; Reis, Rodrigo Arantes; Pedrosa, F. O.; Souza, Emanuel Maltempi de; Monteiro, Rose A.; Baura, Valter A.; Balsanelli, Eduardo; Cruz, Leonardo M.

doi:10.1016/j.scitotenv.2018.07.094

Cited by 27 publications

(12 citation statements)

References 32 publications

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“…Previous work that has focused on mangrove microbial diversity has found that composition of bacterial communities in sediments correlates with a broad range of variables, such as; hydrodynamic regimes, granulometry, organic matter content (Colares and Melo 2013), vegetation (Rocha et al 2016) and pollutant distributions, all of which can generate niche variations (Peixoto et al 2011). However, ecosystems can also exhibit a robust community structure, such that even significant differences in variables, such as pH, are mitigated, resulting in less variation between communities than expected (Huergo et al 2018). Previous work by (Gong et al 2019) found environmental conditions and historical events play an important role in https://doi.org/10.5194/bg-2020-181 Preprint.…”

Section: Discussionmentioning

confidence: 99%

“…While mangrove degradation has long been known to be sensitive to both of these environmental variables (Alongi 2015) these results diverge from others (Rocha et al 2016;Zhang et al 2017), making it difficult to infer general trends. While this work adds to our understanding of prokaryotic variation in mangrove forests, it is important to note that more studies need to be performed in mangroves more diverse than the anthropogenically impacted areas of South America and Asia, as the majority of them have been so far (Imchen et al 2017;Lin et al 2019;Huergo et al 2018;Zhang et al 2018;Gong et al 2019;Ghizelini, Mendonça-Hagler and Macrae 2012).…”

Section: Discussionmentioning

confidence: 99%

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Differential analysis of prokaryotic communities from pristine mangrove tidal zone sediments reveal distinct structures and functional profiles

Santana¹,

Spealman²,

Melo³

et al. 2020

Preprint

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Abstract. Mangrove forests are intertidal ecosystems that constitute a large portion of the world's coastline, as such, they are composed of, and reliant upon, microhabitats defined by the tides. However, we are only beginning to understand tidal microhabitat biodiversity and their role in nutrient cycling. The majority of metagenomic studies have so far been conducted on anthropogenically impacted areas. As even mild disruption can severely alter ecosystems and lead to decreased biodiversity and local extinctions, this is a critical issue. Here, we characterize prokaryotic populations and their involvement in nutrient cycling across the tidal zones of a pristine mangrove forest within a Brazilian Environmental Protection Area of the Atlantic Forest. We hypothesize that tidal zones in pristine mangroves constitute distinct microhabitats, are composed of different prokaryotic communities and, consequently, distinct functional profiles. Samples were collected in triplicate from zones below, between, and above the tidal waterline. Using 16S amplicon sequencing, we find significantly different prokaryotic communities with diverse nutrient cycling related functions, as well specific taxa with varying contribution to functional abundances between zones. Our findings contrast those observed in anthropogenically impacted mangroves and suggest that some aspects of mangrove zonation may be compromised by human activity.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Differential analysis of prokaryotic communities from pristine mangrove tidal zone sediments reveal distinct structures and functional profiles

Santana¹,

Spealman²,

Melo³

et al. 2020

Preprint

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“…The medium layer, namely CM, did not yield enough DNA for this analysis and was not considered for further analysis. The extracted metagenomic DNA from the top layer, namely S, and the bottom layer, namely B, were used as template for PCR ampli cation of the V4 region of the 16S rRNA gene and subjected to next generation Illumina sequencing using primers and methodology described previously (Huergo et al, 2018).…”

Section: Prokaryotic Biodiversity Using 16s Rdna Amplicon Sequencingmentioning

confidence: 99%

“…Still, most of the Brazilian biomes are only marginally explored in terms of microbiome composition (Pylro and Roesch, 2017). Our research group is combining metagenomics and traditional cell culture techniques to uncover the microbiome in yet to be explored Brazilian environments such as the pristine Amazon forest air (Souza et al, 2019) and the mangrove soil (Huergo et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Mangroves are particularly interesting habitats for microbial prospection, given the unique chemical characteristics and diel tidal variations which affect salinity, oxygen, pH and nutrient availability (Alongi, 2014). Nevertheless, there are very few studies on Brazilian mangrove microbiome (Andreote et al, 2012;Ceccon et al, 2019;Huergo et al, 2018; M. and R., 2020; Mendes and Tsai, 2018).…”

Section: Introductionmentioning

confidence: 99%

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The Microbiome of a Shell Mound: Ancient Anthropogenic Waste as a Source of Streptomyces Degrading Recalcitrant Polysaccharides

Huergo

Conzentino

Gonçalves

et al. 2021

Preprint

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Metagenome amplicon DNA sequencing and traditional cell culture techniques are helping to uncover the diversity and the biotechnological potential of prokaryotes in different habitats around the world. It has also had a profound impact on microbial taxonomy in the last decades. Here we used metagenome 16S rDNA amplicon sequencing to reveal the microbiome composition of different layers of an anthropogenic soil collected at a shell mound Sambaqui archeological site. The Samabaqui soil microbiome is mainly composed by phyla Acidobacteria, Rokubacteria, Proteobacteria and Thaumarchaeota. Using culture-dependent analysis we isolated few Streptomyces strains from the Sambaqui soil. One of the isolates, named Streptomyces sp. S3, was able to grow in minimal medium containing recalcitrant polysaccharides including chitin, xylan, carboxymethylcellulose or microcrystalline cellulose as sole carbon sources. The activities of enzymes degrading these compounds were confirmed in cell free supernatants. The genome sequence revealed not only an arsenal of genes related to polysaccharides degradation but also biosynthetic gene clusters which may be involved in the production of biotechnologically interesting secondary metabolites.

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Investigation of the Structural and Functional Microbial Diversity in Indian Mangroves

2019

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Influence of ancient anthropogenic activities on the mangrove soil microbiome

Cited by 27 publications

References 32 publications

Differential analysis of prokaryotic communities from pristine mangrove tidal zone sediments reveal distinct structures and functional profiles

Differential analysis of prokaryotic communities from pristine mangrove tidal zone sediments reveal distinct structures and functional profiles

The Microbiome of a Shell Mound: Ancient Anthropogenic Waste as a Source of Streptomyces Degrading Recalcitrant Polysaccharides

Investigation of the Structural and Functional Microbial Diversity in Indian Mangroves

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