As the highest plateau on the Earth, the Tibetan Plateau (TP) has experienced rapid warming in the last decades, affecting natural ecosystem and water resources extending far beyond the plateau itself. A distinctive characteristic known as elevation-dependent warming (EDW) in the high mountain regions was particularly pronounced in the TP, whereby the magnitude of temperature warming was amplified with increasing altitudes. Different mechanisms have been proposed to explain this phenomenon, however, the link between the root cause of warming, human activities, and the EDW remains poorly understood. Here we used the homogenized observation and simulations by the newest climate models to discern human influence on both mean and extreme temperatures within the region. An optimal fingerprinting method was applied in a vertical space rather than in traditional horizontal space. We found that the long-term trends in mean and extreme temperature amplified with increasing elevation, with larger magnitude of trends at higher elevations. The response to external forcing, primarily driven by human activities, was robustly detected in altitudinal amplification of temperature increase, providing clear evidence of human causes of EDW. As warming increases, the EDW in the region will continue, with more pronounced EDW corresponding to larger magnitude of warming under a high emission scenario. These findings mark the first evidence of human influence on temperature across different vertical altitudes of climate system.