Influence of Antibiotic Pressure on Five Plasmid-based Bioluminescent Gram-negative Bacterial Strains

Wang, Xiwen; Chi, Hang; Li, Qianxue; Li, Wenliang; Li, Jiakuan; Li, Bo; Gao, Wanli; Zhang, Da; Sun, Yu; Liu, Yi; Qu, Han; Wang, Yutian; Li, Zhiping; Xia, Zhang

doi:10.1007/s11307-017-1110-x

Cited by 4 publications

(14 citation statements)

References 17 publications

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“…Some studies have suggested that the correlation between bioluminescence intensity and bacterial load might be changed in mice on different antibacterial treatments as antibiotic pressure can enhance bioluminescence signals [21, 22]. Therefore, caution needs to be applied when directly comparing the bioluminescence intensity between animals on different antibiotic therapies.…”

Section: Discussionmentioning

confidence: 99%

Sustained activity of novel THIOMAB antibody-antibiotic conjugate against Staphylococcus aureus in a mouse model: Longitudinal pharmacodynamic assessment by bioluminescence imaging

Zhou¹,

Cai²,

Baruch³

et al. 2019

PLoS ONE

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Staphylococcus aureus (S. aureus) infections are a leading cause of death by an infectious agent. Survival within host phagocytic cells is one mechanism by which S. aureus evades antibiotic treatment. A novel THIOMAB™ antibody-antibiotic conjugate (TAC) strategy was developed to kill S. aureus intracellularly and mitigate the spread of infection. In this report, we used a longitudinal whole-body bioluminescence imaging method to study the antibacterial dynamics of TAC alone or in combination with vancomycin in a mouse infection model. Injections of stably luminescent S. aureus bacteria into mice resulted in exponential increases in whole body bioluminescence with a reduction in body weight and survival rate. Vancomycin, a standard-of-care antibiotic, suppressed bacterial growth in mice. However, bacterial growth rebounded in these animals once treatment was discontinued. In contrast, single dose of TAC showed rapid reduction of bioluminescence intensity, which persisted for up to 19 days. The combination of TAC and vancomycin achieved a more sustained and significantly greater reduction of bioluminescence compared with vancomycin alone. In summary, the present study showed an imaging method to longitudinally assess antibacterial drug dynamics in mice and demonstrated that TAC monotherapy or in combination with vancomycin had superior and sustained activity compared to vancomycin alone.

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Section: Discussionmentioning

confidence: 99%

Sustained activity of novel THIOMAB antibody-antibiotic conjugate against Staphylococcus aureus in a mouse model: Longitudinal pharmacodynamic assessment by bioluminescence imaging

Zhou¹,

Cai²,

Baruch³

et al. 2019

PLoS ONE

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show abstract

“…10 The recombinant plasmid pBBR-lux and the monitoring of the bioluminescent intensity and stability have been described previously. 6,7 The luciferase-expressing bioluminescent strains were detected using a NightOWL II LB 983 in vivo Imaging System (Berthold Technologies, Bad Wildbad, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…6 The plasmid in E. coli O157:H7 exhibited high stability, which was determined by the ratio between bioluminescence normalized for CFU under nonselective conditions versus kanamycin-selected conditions. 6 …”

Section: Methodsmentioning

confidence: 99%

“…6 In addition, the bacterial luciferase gene cassette (luxCDABE) is an ideal bioreporter for real-time monitoring of the dynamics of bacterial colonization and clearance because it is a fully autonomous, substrate-free bioluminescent reporter system available in a prokaryotic or eukaryotic host background. pBBR-lux is a broad host range plasmid vector that carries the luxCDABE operon for the construction of bioluminescent Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…In a previous study, the current authors found that Escherichia coli O157 could reproduce in the lungs of mice after an intraperitoneal injection, which suggested that this bacterium might be used as a tool to explore the mechanisms of bacterial infection following IAV infection. 6 In addition, the bacterial luciferase gene cassette (luxCDABE) is an ideal bioreporter for real-time monitoring of the dynamics of bacterial colonization and clearance because it is a fully autonomous, substrate-free bioluminescent reporter system available in a prokaryotic or eukaryotic host background. pBBR-lux is a broad host range plasmid vector that carries the luxCDABE operon for the construction of bioluminescent Gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Bacteria meets influenza A virus: A bioluminescence mouse model of Escherichia coli O157:H7 following influenza A virus/Puerto Rico/8/34 (H1N1) strain infection

et al. 2018

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ObjectiveTo develop a bioluminescence-labelled bacterial infection model to monitor the colonization and clearance process of Escherichia coli O157:H7 in the lungs of mice following influenza A virus/Puerto Rico/8/34 (H1N1) strain (IAV/PR8) infection.MethodsBALB/c mice were administered IAV/PR8 or 0.01 M phosphate-buffered saline (PBS; pH 7.4) intranasally 4 days prior to intranasal administration of 1 × 107 colony-forming units (CFU) of E. coli O157:H7-lux. Whole-body bioluminescent signals were monitored at 10 min, 4 h, 8 h, 12 h, 16 h and 24 h post-bacterial infection. Lung bioluminescent signals and bacterial load (CFU/g) were monitored at 4 h, 8 h, 12 h, 16 h and 24 h post-bacterial infection.ResultsPrior IAV/PR8 infection of mice resulted in a higher level of bacterial colonization and a lower rate of bacterial clearance from the lungs compared with mice treated with PBS. There were also consistent findings between the bioluminescence imaging and the CFU measurements in terms of identifying bacterial colonization and monitoring the clearance dynamics of E. coli O157:H7-lux in mouse lungs.ConclusionThis novel bioluminescence-labelled bacterial infection model rapidly detected bacterial colonization of the lungs and monitored the clearance dynamics of E. coli O157:H7-lux following IAV/PR8 infection.

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Recent advance in microbial fuel cell reactor configuration and coupling technologies for removal of antibiotic pollutants

Song

Han

et al. 2022

Current Opinion in Electrochemistry

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Influence of Antibiotic Pressure on Five Plasmid-based Bioluminescent Gram-negative Bacterial Strains

Abstract: The bioluminescent signals and CFU enhancement at sub-MIC antibiotic concentrations should be of value in the research of new antibiotic drugs and bioluminescent imaging.

Cited by 4 publications

References 17 publications

Sustained activity of novel THIOMAB antibody-antibiotic conjugate against Staphylococcus aureus in a mouse model: Longitudinal pharmacodynamic assessment by bioluminescence imaging

Sustained activity of novel THIOMAB antibody-antibiotic conjugate against Staphylococcus aureus in a mouse model: Longitudinal pharmacodynamic assessment by bioluminescence imaging

Bacteria meets influenza A virus: A bioluminescence mouse model of Escherichia coli O157:H7 following influenza A virus/Puerto Rico/8/34 (H1N1) strain infection

Recent advance in microbial fuel cell reactor configuration and coupling technologies for removal of antibiotic pollutants

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