Influence of Antibiotics and Surfactants Addition on Growth and Methionine Productivity by &amp;lt;i&amp;gt;Bacillus cereus&amp;lt;/i&amp;gt;

Dike, K. S.; Ekwealor, I. A.; Eziuzor, S. C.

doi:10.4236/aim.2013.31004

Cited by 3 publications

(1 citation statement)

References 21 publications

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“…Most natural strains cannot produce industrially substantial amounts of L-methionine in the broth culture due to their various metabolic regulation mechanisms. Some attempts have been made to overproduce biologically active L-methionine using fermentation methods [14,15,16,8,10,17,12,13].…”

Section: Introductionmentioning

confidence: 99%

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Ezemba

Ekwealor

2021

MRJI

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Three of the methionine-producing bacteria previously isolated from different soil ecovars in Owerri, characterized as Bacillus cereus no: DS13, RS16 and AS9, based on 16s rRNA sequencing, were screened for utilization agricultural products for production of methionine. Experimental studies on the Bacillus strains were carried out to examine the use of various starch hydrolysates from different agricultural products: wheat, sorghum, cassava, cocoyam, yam, plantain, millet, corn, potato, rice and defatted plant proteins: pigeon pea, cowpea, bambaranut, groundnut and soybean meal as source of carbon and nitrogen, respectively for the production of methionine. The result of the influence of various carbon and nitrogen sources on methionine production showed that 2.32mg/ml methionine accumulation in the culture broth of B. cereus DS13 when corn-starch hydrolysate/defatted groundnut meal was used, while B. cereus RS16 and Bacillus cereus DS13 gave the same methionine yield of 2.22mg/ml with corn-starch hydrolysate/defatted groundnut meal and sorghum hydrolysate/soyabean meal respectively. Bacillus cereus AS9 utilized wheat starch hydrolysate/soaybean meal, followed by plaintain-starch hydrolysate/bambaranut to produced 1.79mg/ml and 1.78mg/ml methionine respectively. Methionine was accumulated in the fermentation broth of the Bacillus strains after a fermentation period of 96h. This study indicates the possible utilization of agricultural products as substrates for L-methionine production in submerged medium especially when considering the limitations of producing methionine by fermentation processes.

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Section: Introductionmentioning

confidence: 99%

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Ezemba

Ekwealor

2021

MRJI

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Reduction of nitrogen excretion and emission in poultry: A review for organic poultry

Chalova

Kim

Patterson

et al. 2016

Journal of Environmental Science and Health, Part B

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Organic poultry is an alternative to conventional poultry which is rapidly developing as a response to customers' demand for better food and a cleaner environment. Although organic poultry manure can partially be utilized by organic horticultural producers, litter accumulation as well as excessive nitrogen still remains a challenge to maintain environment pureness, animal, and human health. Compared to conventional poultry, diet formulation without nitrogen overloading in organic poultry is even more complicated due to specific standards and regulations which limit the application of some supplements and imposes specific criteria to the ingredients in use. This is especially valid for methionine provision which supplementation as a crystalline form is only temporarily allowed. This review is focused on the utilization of various protein sources in the preparation of a diet composed of 100% organic ingredients which meet the avian physiology need for methionine, while avoiding protein overload. The potential to use unconventional protein sources such as invertebrates and microbial proteins to achieve optimal amino acid provision is also discussed.

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Lysine Production of <i>Microbacterium lacticum</i> by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources

Ezemba¹,

Ozokpo²,

Anakwenze³

et al. 2016

AiM

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Bacterial isolation from oil-contaminated and uncontaminated soil was screened for hydrocarbon utilizer which was also capable of producing lysine. Microbial production of lysine by Microbacterium lacticum was investigated in submerged fermentations using various concentrations of hydrocarbon, sugar sources and nitrogen. Of the nine sugar and five nitrogen sources tested, glucose/ammonium sulphate proved optimum for lysine production. Effect of varying concentration of carbon and nitrogen sources on lysine accumulation showed that glucose (4%) ammonium sulphate (1%) respectively increased lysine production. A gram positive rod bacterium identified as Microbacterium lacticum was identified. Optimizing the cultural conditions of Microbacterium lacticum in submerged medium gave a methionine yield of 2.99 mg/ml lysine in the broth culture after 96 h.

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Influence of Antibiotics and Surfactants Addition on Growth and Methionine Productivity by <i>Bacillus cereus</i>

Cited by 3 publications

References 21 publications

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Reduction of nitrogen excretion and emission in poultry: A review for organic poultry

Lysine Production of <i>Microbacterium lacticum</i> by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources

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Influence of Antibiotics and Surfactants Addition on Growth and Methionine Productivity by &lt;i&gt;Bacillus cereus&lt;/i&gt;

Cited by 3 publications

References 21 publications

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Utilization of Various Starch Hydrolysates and Defatted Proteins by Bacillus cereus for Microbial Synthesis of Methionine in Submerged Medium

Reduction of nitrogen excretion and emission in poultry: A review for organic poultry

Lysine Production of &lt;i&gt;Microbacterium lacticum&lt;/i&gt; by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources

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Product

Resources

About

Influence of Antibiotics and Surfactants Addition on Growth and Methionine Productivity by <i>Bacillus cereus</i>

Lysine Production of <i>Microbacterium lacticum</i> by Submerged Fermentation Using Various Hydrocarbon, Sugar and Nitrogen Sources