Influence of Aryl—Containing Xenobiotics Concentration on the Oxygenase Enzyme Activities

Topalova, Yana; Dimkov, R.; Manolov, R.

doi:10.1080/13102818.1994.10818755

Cited by 7 publications

(10 citation statements)

References 12 publications

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“…For determination of enzyme activities of the samples, cell-free extract was produced by the Feist and Hegeman [ 14 ] method modification of Topalova et al[ 15 ]…”

Section: Methodsmentioning

confidence: 99%

Microbial community development of biofilm in Amaranth decolourization technology analysed by FISH

Belouhova

Schneider

Chakarov

et al. 2014

Biotechnology & Biotechnological Equipment

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The aim of this study was to elucidate the role, the space distribution and the relationships of the bacteria from the genus Pseudomonas in a biofilm community during semi-continuous Amaranth decolourization process in model sand biofilters. The examined parameters of the process were as follows: technological parameters; key enzyme activities (azoreductase, succinate dehydrogenase, catechol-1,2-dioxygenase, catechol-2,3-dioxygenase); the number of azo-degrading bacteria and the bacteria from genus Pseudomonas (plate count technique); the amount and the location of Pseudomonas sp. using fluorescent in situ hybridization (FISH).The results showed that the increase of the Amaranth removal rate with 120% was accompanied with increase of the enzyme activities of the biofilm (azoreductase activity – with 25.90% and succinate dehydrogenase – with 10.61%). The enzyme assays showed absence of activity for сatechol-1,2-dioxygenase and catechol-2,3-dioxygenase at the early phase and high activities of the same oxygenases at the late phase (2.76 and 1.74 μmol/min mg protein, respectively). In the beginning of the process (0–191 h), the number of the culturable microorganisms from genus Pseudomonas was increased with 48.76% but at the late phase (191–455 h) they were decreased with 15.25% while the quantity of the non-culturable bacteria from this genus with synergetic relationships was increased with 23.26%.The dominant microbial factors were identified in the structure of the biofilm during the azo-degradation process by using FISH analysis. Furthermore, the inner mechanisms for increase of the rate and the range of the detoxification were revealed during the complex wastewater treatment processes.

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“…For determination of enzyme activities of the samples, cell-free extract was produced by the Feist and Hegeman [ 14 ] method modification of Topalova et al[ 15 ]…”

Section: Methodsmentioning

confidence: 99%

Microbial community development of biofilm in Amaranth decolourization technology analysed by FISH

Belouhova

Schneider

Chakarov

et al. 2014

Biotechnology & Biotechnological Equipment

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“…The investigated bacterial strain cleavages the phenol via the orthomechanism and a key metabolite catechol (Cl2DO). Besides this main mechanism, in a high concentration of the toxic substrate and high rate of phenol detoxification the role of the meta-mechanism via a key metabolite catechol (C23DO) and ortho-mechanism via a key metabolite protocatechate (P34DO) as additional and alternative pathways of speeding up of the phenol catabolism were enhanced (14). The obtained results are presented in Fig.…”

Section: Resultsmentioning

confidence: 55%

“…The crude cells extracts were prepared by sonic disruption of the bacteria method (5) modified by us (14). Catechol 1,2-tlioxygenase (EC 1.13.11.1 I Cl2DO) activity was determined after method of Willets and Cain (18).…”

Section: Methodsmentioning

confidence: 99%

Two Steps for Speeding up of Phenol Detoxification

Topalova

Dimkov

Merdjanov

1995

Biotechnology & Biotechnological Equipment

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“…This is in accordance with the above conclusion that the ND had a stimulating effect in the initial phase of phenol biodegradation. It should be noted that in other studies the role of glucose as a co-metabolite repressor and inhibitor for the 55 With bold are indicated the key enzymes that in this paper have been investigated).…”

Section: Enzyme Activities Of Pseudomonas Sp In Phenolmentioning

confidence: 93%

“…The result from the benzene ring cleavage, as well as from the several consecutive transformation reactions from the third stage is key metabolic products of cycle of tricarboxylic acids (CTA) and a good enzyme indicator for the rate of the functioning of the CTA is succinate dehydrogenase. 55 In studying the catabolic mechanisms of detoxification processes, the effectiveness of phenol removal and the specific rate of biodegradation were analyzed in parallel (Table II). The key enzyme activities directly related to the biodegradation of the phenol (C12DO, C23DO, P34DO, SDH) were measured too (Table III).…”

Section: Enzyme Activities Of Pseudomonas Sp In Phenolmentioning

confidence: 99%

The Effect of Nanodiamonds on Phenol Biodegradation by <I>Pseudomonas sp</I>. Strain Isolated from Polluted Sediments

Yotinov¹,

Todorova²,

Irina³

et al. 2016

j nanosci nanotechnol

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Carbon nanoparticles, which include nanodiamonds (ND), are used in a number of technological and medical applications. The influence of ND on the mechanisms and regulation of the degradation of xenobiotics in bioremediation technologies and in particular the biodegradation of aromatic hydrocarbons, however, remains unexplored. The aim of this paper is to investigate the effect of ND on the biodegradation of phenol in model conditions. The process of degradation was realized with a microbial culture of Pseudomonas sp. isolated from contaminated sediments. It was found that ND had a stimulating effect on the initial phase of biodegradation of phenol. ND led to an increase in all important parameters of biological detoxification-rate of phenol biodegradation by 154.86%, effectiveness of phenol detoxification by 151.15%. The stimulating effect was also found with regard to the enzyme activity of key oxygenases responsible for the complete degradation of phenol-phenol 2-monooxygenase, catechol-1,2-dioxygenase, catechol-2,3-dioxygenase. The study has proved that nanodiamonds can be used as a potential nanoregulator of detoxification and biodegradation processes.

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Influence of Aryl—Containing Xenobiotics Concentration on the Oxygenase Enzyme Activities

Cited by 7 publications

References 12 publications

Microbial community development of biofilm in Amaranth decolourization technology analysed by FISH

Microbial community development of biofilm in Amaranth decolourization technology analysed by FISH

Two Steps for Speeding up of Phenol Detoxification

The Effect of Nanodiamonds on Phenol Biodegradation by <I>Pseudomonas sp</I>. Strain Isolated from Polluted Sediments

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