Influence of ascorbic acid supplementation on copper status in young adult men

Finley, Elizabeth; Cerklewski, Florian L.

doi:10.1093/ajcn/37.4.553

Cited by 75 publications

(21 citation statements)

References 40 publications

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“…Previous studies on laboratory animals and young humans reported that with high dose of vitamin C intake decreased the levels of serum ceruloplasmin. 24,25 Findings of this study is not in agreement with the previous study ; which may be due to the difference in the dosage levels. In the present study the dosage used was 20mg/kg b.w.…”

Section: Discussioncontrasting

confidence: 84%

Effect of Antioxidants (Vitamin C) on Tissue Ceruloplasmin Following Renal Ischemia Reperfusion in Wistar Rats

Vinodini¹,

Tripathi²,

Raghuveer³

et al. 2012

Int J of Biomed & Adv Res

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Oxidant injury has been implicated in the pathogenesis of inflammatory, metabolic and toxic insults in ischemic -reperfusion injury. Oxidant injury is initiated by free radicals and reactive oxygen molecules .However when the cells are exposed to oxidant stress several different antioxidant defense systems operate to prevent or limit the injury. Ceruloplasmin one among such antioxidants; a powerful free radical scavenger. Aim: To study the effect of vitamin C on tissue ceruloplasmin level following renal reperfusion. Materials and Methods:Wistar albino rats were divided into Group I, II & III the Gr. II the experimental groups) were subjected to ischemia for 60 minutes followed by 24 hrs of reperfusion. The Gr.III was pre-treated with vitamin C (20mg/kg.bw) for 30 days followed by 60 minute ischemia & 24hrs of reperfusion. After the experimental procedure was over; the kidneys were removed and homogenized. The homogenized tissue was used for biochemical estimation of lipid peroxidation & ceruloplasmin. Result: A significant in the levels of tissue lipid peroxidation (MDA) and increase in tissue ceruloplasmin level was observed in Group II compared to those in Group I (normal control) However, the pre-treated group (Group III) showed an increase in the levels of ceruloplasmin and a decrease in lipid peroxidation in comparison to group II. Conclusion:The results of the present study suggest that administration of vitamin C prior to renal ischemia reperfusion protect the renal tissue from the free radical induced reperfusion injury.

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Section: Discussioncontrasting

confidence: 84%

Effect of Antioxidants (Vitamin C) on Tissue Ceruloplasmin Following Renal Ischemia Reperfusion in Wistar Rats

Vinodini¹,

Tripathi²,

Raghuveer³

et al. 2012

Int J of Biomed & Adv Res

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“…Apart from some genetic disorders, leukemias, and lymphoproliferative disorders (36), the only known cause of reduced ESOD activity is copper depletion, and in studies of copperdepleted adults (1 1, 12) or those receiving zinc supplements (10, 13) a reduction of ESOD activity was the earliest indicator of disturbed copper metabolism. Although high doses of ascorbic acid can alter the metabolism of copper in rats (37) and adult men (38,39), the doses of ascorbate used in this study were similar in all treatment groups. Similarly, solids contributed only a small amount to the dietary intake of 15 infants and would take longer than 2 wk to have any effect on ESOD activity.…”

Section: Discussionmentioning

confidence: 79%

Reduced Erythrocyte Superoxide Dismutase Activity in Low Birth Weight Infants Given Iron Supplements

et al. 1991

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ABSTRAU. Erythrocyte superoxide dismutase (ESOD) activity reflects copper utilization and the risk of copper deficiency. To investigate the possible effects of inorganic iron on the metabolism of copper in low birth weight infants, we have measured ESOD activities in three groups of infants receiving different iron supplements. Fifty-five low birth weight infants were randomly assigned to receive daily from 28 d either 13.8 mg (HiFe), 7 mg (MidFe), or no elemental iron (NatFe) as iron edetate. At 27 d, 8, 12, and 20 wk postnatal age, infants were weighed and measured and hematologic indices, plasma ferritin, zinc, and copper concentrations, and ESOD activities were assayed. Anthropometrical and hematologic indices and plasma copper and zinc concentrations did not differ among treatment groups at any time, but at 20 wk, plasma ferritin concentrations [(pg/L) mean; SD] were lower in the NatFe group (17; 2.0) than in the HiFe group (32; 1.9: 95% confidence interval for mean difference 6.6 to 22.0, p < 0.01). ESOD activities (U/g Hb) were similar in HiFe (1447; 263), MidFe (1552; 322), and NatFe (1538; 382) groups at 27 d, but by 20 wk activities in the HiFe group (1537; 211) were lower than in the MidFe (1789; 403: 95% confidence interval 38 to 466, p < 0.05) and NatFe (1858; 304: 95% confidence interval 150 to 492,p < 0.01) groups. The lower ESOD activities found in the HiFe group at 20 wk may reflect altered copper metabolism induced by the iron supplement, but the clinical importance of this observation is unknown. (Pediatr Res 29: 297-301,1991) Abbreviations ESOD, erythrocyte superoxide dismutase CI, confidence interval HiFe, high iron intake LBW, low birth weight MidFe, middle iron intake NatFe, natural iron intake SOD, superoxide dismutase RBC, red blood cell Because iron and copper have similar physicochemical properties, interactions can occur between them that can affect adversely the metabolism of copper (1). The practical relevance of this has been shown in mixed fed healthy infants in whom an iron-fortified (10.2 mg/L) formula achieved a lower intestinal absorption and whole body retention of copper than did the

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“…Its interaction with the copper I1 (cupric) ion is well known, but the in vivo biological consequences of this interaction are less well understood. Previous studies have explored some aspects of this interaction in guinea-pigs (Hitier, 1976;Milne & Omaye, 1980;Smith & Bidlack, 1980;Kassouny et al 1985;DiSilvestro, 1986;Pekiner & Nebioglu, 1994); in rats (Van Campen & Gross, 1968;Johnson & Murphy, 1988;van den Berg et al 1994); in rabbits (Hunt & Carlton, 1965); in chicks (Starcher et al 1964;Carlton & Henderson, 1965;Hill & Starcher, 1965;DiSilvestro & Harris, 1981); in monkeys (Milne et al 1981) and in human subjects (Finley & Cerklewski, 1983;Jacob et al 1987;Milne et al 1988;Pekiner & Nebioglu, 1994). There have also been studies in cultured cells (Harris & Percival, 1991 .…”

mentioning

confidence: 99%

Vitamin C and copper interactions in guinea-pigs and a study of collagen cross-links

Tsuchiya

Bates

1997

Br J Nutr

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While it is clear from these studies that high concentrations or intakes of ascorbate can reduce tissue concentrations of Cu in a variety of species, there is a less clear consensus and uniformity with respect to possible functional effects of ascorbate overload on Cudependent processes in viva Several early studies on aortic rupture in chicks (Starcher et al. 1964;Carlton & Henderson, 1965;Hill & Starcher, 1965) obtained strong evidence for a gross failure of elastin cross-linking, attributed to reduced activity of the key enzyme, lysyl oxidase, in birds that were exposed simultaneously to excessive dietary ascorbate, and to low dietary Cu levels. Studies in mammalian species, however, have not reproduced such a dramatic effect. Nevertheless, in view of the potential importance of this interaction, for human subjects who choose to take daily megadoses of ascorbic acid, there is a need for further studies in relevant animal models such as the guinea-pig, which like humans has an absolute requirement for dietary ascorbate, as well as for Cu. The possibility that high levels of circulating vitamin C may increase the likelihood of Fe acting pro-oxidatively, and thereby influence outcome, has been illustrated by recent studies of human pre-term infants (Silvers et al. 1994;Powers et al. 1995). Deleterious effects of high ascorbate intakes may thus involve more than one transition metal.Another separate but related question arises from the need for better biochemical tests for suboptimum v. optimum status, with respect to those functionally-critical processes which are dependent on specific micronutrients. Collagen cross-linking is such a process. A severe lack of Cu can impair the activity of the Cu-dependent enzyme lysyl oxidase, responsible for the initiation of cross-linking (Farquharson et al. 1989;Robins, 1994). Impairment of cross-linking may then result in loss of tensile strength. Lack of ascorbic acid is well known to affect the hydroxylation of collagen lysyl residues (Kivirikko & Myllyla, 1982;Yeowell et al. 1995), and this could, in theory, alter the pattern of collagen cross-links, by altering the ratio of deoxypyridinoline (derived from lysine) to pyridinoline (derived from hydroxylysine) (Robins, 1994).The dual purpose of the present study was: first to re-examine the interactions of ascorbate and Cu, by dietary modulation in young guinea-pigs, and second, to test the hypothesis that Cu and/or ascorbate status might alter the ratio of deoxypyridinoline collagen-derived cross-links in bone and in urine. MATERIALS AND METHODS Animals and dietsThe purified guinea-pig diet contained the following components (g/kg): sucrose 331, maize starch 50, ovalbumin 300, cellulose powder 150, maize oil 73, potassium acetate 25, choline chloride 2, magnesium oxide 5 , inositol2, salt mixture 60, vitamins (see later). The salt mixture was based on that of Greenfield et al. (1969), except that the CuS04 therein was omitted. It contained (g/kg): CaC03 205, CaHP04 325, Na2HP04 185, KC1 205, MgS04 70, MnS04 4-5, Fe-citrate...

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Influence of ascorbic acid supplementation on copper status in young adult men

Cited by 75 publications

References 40 publications

Effect of Antioxidants (Vitamin C) on Tissue Ceruloplasmin Following Renal Ischemia Reperfusion in Wistar Rats

Effect of Antioxidants (Vitamin C) on Tissue Ceruloplasmin Following Renal Ischemia Reperfusion in Wistar Rats

Reduced Erythrocyte Superoxide Dismutase Activity in Low Birth Weight Infants Given Iron Supplements

Vitamin C and copper interactions in guinea-pigs and a study of collagen cross-links

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