Influence of ashing techniques on the analysis of trace elements in animal tissue

Clegg, Michael S.; Keen, Carl L.; Lönnerdal, Bo; Hurley, Lucille S.

doi:10.1007/bf02990451

Cited by 437 publications

(117 citation statements)

References 8 publications

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“…Zinc Analysis-Plasma, tissue, and milk were digested in nitric acid, and zinc concentrations were determined by atomic absorption spectrometry as previously described (23).…”

Section: Methodsmentioning

confidence: 99%

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Lee

Hennigar

Alam

et al. 2015

Journal of Biological Chemistry

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Background: ZnT2 is expressed in non-secreting and secreting mammary epithelium; however, the physiological role is not understood. Results: ZnT2-null mice have impaired mammary expansion and compromised mammary differentiation and milk secretion during lactation. Conclusion: ZnT2-mediated zinc transport is critical for mammary development and function during lactation. Significance: This study identifies novel consequences of ZnT2 function in the mammary gland.

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“…Zinc Analysis-Plasma, tissue, and milk were digested in nitric acid, and zinc concentrations were determined by atomic absorption spectrometry as previously described (23).…”

Section: Methodsmentioning

confidence: 99%

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Lee

Hennigar

Alam

et al. 2015

Journal of Biological Chemistry

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“…After wet-ashing of tissues with nitric and perchloric acid, the liver and kidney tissue Zn, Cu, Fe and Mg levels were studied by atomic absorption spectrophotometry (Shimadzu AA-680, Japan) with an air-acetylene flame. The tissue concentrations of element are expressed as micrograms per gram of wet weight (Clegg et al 1981).…”

Section: The Tissue Element Analysismentioning

confidence: 99%

Evaluation of trace elements and oxidative stress levels in the liver and kidney of streptozotocin-induced experimental diabetic rat model

Özçelik¹,

Tunçdemi̇r²,

Öztürk³

et al. 2012

gpb

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Abstract. In this study, we aimed to investage the relationship among trace elements (Cu, Fe, Zn and Mg) on oxidative and anti-oxidative substances in liver and kidneys tissues in streptozotocin (STZ) diabetic rat model. The mean levels of Fe and Cu were found significantly higher in the liver and kidneys of the diabetic rats, in comparison to the control rats. On the other hand, the mean levels of Zn and Mg in the liver and kidneys of the diabetic rats were significantly lower than in the control rats.The liver and kidneys malonaldehyde (MDA) levels of the experimental group were found to be higher than in the control group (p < 0.001; p < 0.01, respectively) after 4 weeks of the experimental period. Superoxide dismutase (SOD) activities and glutathione (GSH) levels in the liver tissue of STZ-induced diabetic rats were found to be lower in the experimental group than in the control group (p < 0.01). SOD activity and GSH concentration in kidneys of the diabetic rats were significantly diminished with respect to the control group (p < 0.01). In conclusion, the present results indicate that the increase of Fe and Cu together with decreas of Zn and Mg concentration in liver and kidney of STZ-induced diabetic rats may be involved in disturbances of oxidative balance in both the tissues. Therefore, these findings may contribute to explain the role of impaired ion metabolism of some elements in the progression of diabetic oxidative complications.

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“…(1 mL), and liver samples (-0.3 g) were wet ashed with 16 N nitric acid (2 mL of Ultrex grade, J. T. Baker Co., SanFrancisco, CA), concentrated by evaporation and diluted with distilled deionized water as described by Clegg et al (16). Trace element concentrations were determined in the diluted ashed samples by flame atomic absorption spectrophotometry (model IL 55 1, Instrumentation Laboratories, Wilmington, MA).…”

Section: Mt Metallothioneinmentioning

confidence: 99%

Influence of Marginal Maternal Zinc Deficiency on Pregnancy Outcome and Infant Zinc Status in Rhesus Monkeys

Lönnerdal

et al. 1989

Pediatr Res

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ABSTRACT. To investigate the effects of marginal zinc deficiency on early development, rhesus monkeys were fed a diet marginally deficient in zinc (M, 4 pglg) throughout pregnancy and during the first month of lactation. Despite the low concentration of zinc in the diet, M dams did not develop overt signs of zinc deficiency. However, compared to control dams fed diets adequate in zinc (C; 100 pg Zn/ g), M dams showed a low response to the mitogens concanavalin A and phytohemagglutinin. Pregnancy outcome was similar in the two groups and all of the neonates were judged to be healthy at delivery. From birth until d 30 of age, the infants were closely monitored for signs of zinc deficiency, and at d 30, they were killed and tissues were removed and analyzed for a number of parameters reported to be affected by zinc status. At birth, M infants had low plasma zinc concentrations compared to controls; however, this difference was not observed at d 30. D 30 M infants showed a normal response to the mitogens concanavalin A and phytohemagglutinin, but showed a low response to pokeweed mitogen. Tissue (liver, brain, spleen, kidney, and heart) trace element concentrations were similar in the two groups of infants, as were liver metallothionein concentrations and @Zn uptakelretention by isolated hepatocytes. Infant wt gain was inversely correlated with plasma zinc, liver zinc, and liver metallothionein concentrations in both the M and C groups. These results demonstrate that feeding a diet containing 4 fig zinclg to rhesus monkeys during pregnancy and lactation does not result in marked signs of zinc deficiency, although subtle signs occur in both the mother and infant. The data also support the concept that infant growth is associated with a depletion of tissue zinc stores. (Pediatr Res 26:470-477, 1989)

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Influence of ashing techniques on the analysis of trace elements in animal tissue

Cited by 437 publications

References 8 publications

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Evaluation of trace elements and oxidative stress levels in the liver and kidney of streptozotocin-induced experimental diabetic rat model

Influence of Marginal Maternal Zinc Deficiency on Pregnancy Outcome and Infant Zinc Status in Rhesus Monkeys

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Influence of ashing techniques on the analysis of trace elements in animal tissue

Cited by 437 publications

References 8 publications

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Essential Role for Zinc Transporter 2 (ZnT2)-mediated Zinc Transport in Mammary Gland Development and Function during Lactation

Evaluation of trace elements and oxidative stress levels in the liver and kidney of streptozotocin-induced experimental diabetic rat model

Influence of Marginal Maternal Zinc Deficiency on Pregnancy Outcome and Infant Zinc Status in Rhesus Monkeys

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Product

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Evaluation of trace elements and oxidative stress levels in the liver and kidney of streptozotocin-induced experimental diabetic rat model