Influence of Bacterial Contamination and Antibiotic Sensitivity on Cryopreserved Sperm Quality of Indian Red Jungle Fowl

Rakha, Bushra Allah; Zafar, Zartasha; Ansari, Muhammad Tayyab; Akhter, Shamim; Qadeer, Saima; Akhter, Ali; Waseem, Kainat; Santiago-Moreno, J.

doi:10.1089/bio.2022.0029

Cited by 4 publications

(9 citation statements)

References 40 publications

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“…Free radicals pose serious threats to sperm DNA and cause formation of oxidative by‐product, i.e., 8‐oxo‐7, 8‐dihydro‐2′‐deoxyguanosine that induce breaks in the DNA developing a mutagenic effect (Ménézo et al., 2007). These DNA breaks are detrimental in terms of post‐fertilization and embryonic DNA expression (Zuha et al., 2023). In the current study, sperm DNA fragmentation is reported minimal ( p < .05) at 0.4 mM GSH compared to other concentrations and control throughout the stages of cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

“…However, concentrations of GSH above 0.4 mM showed gradual decline in the semen quality and free radical scavenging capacity. This pattern of declining biochemical activity with increasing GSH concentrations after 0.4 mM may reflect that supplemented levels have exceeded the physiological GSH levels required for the ring‐necked pheasant semen (Ansari et al., 2021; Zuha et al., 2023).…”

Section: Discussionmentioning

confidence: 99%

“…Reduced glutathione (GSH) is a natural redox force immediately consumed by cells to oppose toxins and fatty acid oxidation (Zuha et al., 2023). It, being composed of cysteine, glutamate and glycine, maintains proteins and other chief cellular constituents (Dickinson & Forman, 2002; Luberda, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…It, being composed of cysteine, glutamate and glycine, maintains proteins and other chief cellular constituents (Dickinson & Forman, 2002; Luberda, 2005). The GSH is converted to oxidized glutathione (GSSG) in the process and later recycled to a reduced state (GSH) (Ansari et al., 2021; Zuha et al., 2023). Natural GSH level is reported to diminish up to 78% during sperm cryopreservation (Bilodeau et al., 2000), spreading out a wave of partially reduced oxygen radicals in the semen.…”

Section: Introductionmentioning

confidence: 99%

“…Ring‐necked pheasant ( Phasianus colchicus ), brightly coloured gallinaceous bird having well developed resistance to parasites and other diseases makes it highly manageable and craved as game bird and table fare (Dahlgren, 1988; Mateos, 1998). Unfortunately, shifts from conventional to modern farming have jeopardized the existence of this valuable species in the wild due to its dependence on croplands and open grasslands (Zuha et al., 2023). Under the USDA conservation reserve program, efforts have been made to establish the pheasant populations in some areas through intensive habitat management; however, persistent endeavours are required to conserve and propagate this valued species (Matthews et al., 2012).…”

Section: Introductionmentioning

confidence: 99%

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Effect of reduced glutathione on quality, lipid peroxidation and antioxidant potential of frozen‐thawed ring‐necked pheasant semen

Zuha,

Rakha,

Akhter

et al. 2024

Reprod Domestic Animals

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Reduced glutathione (GSH) is a natural redox force immediately consumed by cells to combat toxins. Today's world is shifting towards clean farming practices whence ring‐necked pheasants dependent on croplands are facing threats and sperm‐assisted reproductive techniques become indispensable in preserving this valuable species for game and meat purposes. This study aims at evaluating the cryosurvivability (motility, viability, plasma membrane and acrosomal integrity, DNA fragmentation) and antioxidant capacity (Free radical scavenging activity, LPO detection, Ferric reducing antioxidant power, mitochondrial status) of ring‐necked pheasant semen with GSH supplementation in the extender. Semen collected from ten males was pooled, divided and diluted with Beltsville poultry semen extender (1:5) containing 0.0, 0.2, 0.4, 0.6 and 0.8 mM GSH. Samples were cryopreserved, and sperm quality was evaluated post‐dilution, cooling, equilibration, and freeze–thaw. Free radical scavenging activity, total antioxidant potential, mitochondrial action and lipid peroxidation were assessed at post‐thawing. Sperm motility, plasma membrane and acrosomal integrity, viability and DNA integrity were recorded highest (p < .05) at 0.4 mM GSH compared to other concentrations and control at all the stages of cryopreservation. Similarly, sperm mitochondrial action, antioxidant potential and free radical scavenging capacity were recorded higher (p < .05) with 0.4 mM GSH at post‐thaw compared to other concentrations of GSH and control. Lipid peroxidation was recorded as highest (p < .05) in the control compared to GSH‐supplemented groups at post‐thaw. Hence, it is concluded that GSH at 0.4 mM preserves the quality and ROS scavenging capacity of cryopreserved ring‐necked pheasant sperm as an antioxidant.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effect of reduced glutathione on quality, lipid peroxidation and antioxidant potential of frozen‐thawed ring‐necked pheasant semen

Zuha,

Rakha,

Akhter

et al. 2024

Reprod Domestic Animals

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Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

Hu,

Li,

Xin

et al. 2024

Poultry Science

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Short-Term Storage of Rooster Ejaculates: Sperm Quality and Bacterial Profile Differences in Selected Commercial Extenders

et al. 2023

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Bacterial contamination of semen has become an important contributor to the reduced shelf life of insemination doses in the poultry industry, which is why antibiotics (ATBs) are an important component of semen extenders. Due to a global rise in antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available semen extenders to prevent possible bacterial contamination of rooster ejaculates. Two selected extenders free from or containing 31.2 µg/mL kanamycin (KAN) were used to process semen samples from 63 healthy Lohmann Brown roosters. Phosphate-buffered saline without ATBs was used as a control. The extended samples were stored at 4 °C for 24 h. Sperm motility, viability, mitochondrial activity, DNA integrity and the oxidative profile of each extended sample were assessed following 2 h and 24 h of storage. Furthermore, selective media were used to quantify the bacterial load and specific bacterial species were identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicate that semen extenders enriched with KAN ensured a significantly higher preservation of sperm quality in comparison to their KAN-free counterparts. Bacterial load was significantly decreased in diluents supplemented with ATBs (p ≤ 0.001); however, KAN alone was not effective enough to eradicate all bacteria since several Escherichia coli, Enterococcus faecalis, Enterococcus faecium and Micrococcus luteus were retrieved from samples extended in KAN-supplemented commercial extenders. As such, we may suggest that more focus should be devoted to the selection of an optimal combination and dose of antibiotics for poultry extenders, which should be accompanied by a more frequent bacteriological screening of native as well as extended poultry semen.

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Influence of Bacterial Contamination and Antibiotic Sensitivity on Cryopreserved Sperm Quality of Indian Red Jungle Fowl

Cited by 4 publications

References 40 publications

Effect of reduced glutathione on quality, lipid peroxidation and antioxidant potential of frozen‐thawed ring‐necked pheasant semen

Effect of reduced glutathione on quality, lipid peroxidation and antioxidant potential of frozen‐thawed ring‐necked pheasant semen

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

Short-Term Storage of Rooster Ejaculates: Sperm Quality and Bacterial Profile Differences in Selected Commercial Extenders

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