Influence of bariatric surgery induced weight loss on oxidative DNA damage

Bankoglu, Ezgi Eyluel; Gerber, Johanna; Kodandaraman, Geema; Seyfried, Florian; Stopper, Helga

doi:10.1016/j.mrgentox.2020.503194

Cited by 14 publications

(18 citation statements)

References 28 publications

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Section: Prevention or Reversal Of Obesity-induced Adipose Dysfunctiosupporting

confidence: 80%

“…In a randomized controlled trial where participants were placed on a 6-month calorie restriction regimen, a significant decrease in DNA damage measured in blood by the comet assay was reported (167). Similar reductions in DNA damage have also been reported in blood and in lymphocytes of morbidly obese patients after weight loss surgery (168)(169)(170). Setayesh et al showed in a mouse model of diet-induced obesity that weight loss significantly decreased DNA damage in the colon, liver, and testes in association with reductions in circulating levels of IL-6, MCP-1, leptin, and TNF and increased adiponectin (171).…”

Section: Targeting Obesity-induced Dna Damagementioning

confidence: 63%

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Obese Adipose Tissue as a Driver of Breast Cancer Growth and Development: Update and Emerging Evidence

Bhardwaj

Brown

2021

Front. Oncol.

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Obesity is an established risk factor for breast cancer growth and progression. A number of advances have been made in recent years revealing new insights into this link. Early events in breast cancer development involve the neoplastic transformation of breast epithelial cells to cancer cells. In obesity, breast adipose tissue undergoes significant hormonal and inflammatory changes that create a mitogenic microenvironment. Many factors that are produced in obesity have also been shown to promote tumorigenesis. Given that breast epithelial cells are surrounded by adipose tissue, the crosstalk between the adipose compartment and breast epithelial cells is hypothesized to be a significant player in the initiation and progression of breast cancer in individuals with excess adiposity. The present review examines this crosstalk with a focus on obese breast adipose-derived estrogen, inflammatory mediators and adipokines, and how they are mechanistically linked to breast cancer risk and growth through stimulation of oxidative stress, DNA damage, and pro-oncogenic transcriptional programs. Pharmacological and lifestyle strategies targeting these factors and their downstream effects are evaluated for feasibility and efficacy in decreasing the risk of obesity-induced breast epithelial cell transformation and consequently, breast cancer development.

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Section: Prevention or Reversal Of Obesity-induced Adipose Dysfunctiosupporting

confidence: 80%

Section: Targeting Obesity-induced Dna Damagementioning

confidence: 63%

Obese Adipose Tissue as a Driver of Breast Cancer Growth and Development: Update and Emerging Evidence

Bhardwaj

Brown

2021

Front. Oncol.

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“…For this aim, we used 20 anonymized blood samples. These were remaining blood volumes from studies with University Wuerzburg ethical approval numbers 186/14 and 202/19, partially published in (Bankoglu et al 2020 , 2018 ), which were used fresh and also cryopreserved as whole blood or isolated peripheral blood mononuclear cells. Whole blood samples were aliquoted as 250 µl portions and frozen without cryoprotectant.…”

Section: Methodsmentioning

confidence: 99%

Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay

et al. 2021

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The comet assay is a commonly used method to determine DNA damage and repair activity in many types of samples. In recent years, the use of the comet assay in human biomonitoring became highly attractive due to its various modified versions, which may be useful to determine individual susceptibility in blood samples. However, in human biomonitoring studies, working with large sample numbers that are acquired over an extended time period requires some additional considerations. One of the most important issues is the storage of samples and its effect on the outcome of the comet assay. Another important question is the suitability of different blood preparations. In this study, we analysed the effect of cryopreservation on DNA damage and repair activity in human blood samples. In addition, we investigated the suitability of different blood preparations. The alkaline and FPG as well as two different types of repair comet assay and an in vitro hydrogen peroxide challenge were applied. Our results confirmed that cryopreserved blood preparations are suitable for investigating DNA damage in the alkaline and FPG comet assay in whole blood, buffy coat and PBMCs. Ex vivo hydrogen peroxide challenge yielded its optimal effect in isolated PBMCs. The utilised repair comet assay with either UVC or hydrogen peroxide-induced lesions and an aphidicolin block worked well in fresh PBMCs. Cryopreserved PBMCs could not be used immediately after thawing. However, a 16-h recovery with or without mitotic stimulation enabled the application of the repair comet assay, albeit only in a surviving cell fraction.

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“…In essence, Fpg converts undetectable oxidized base lesions into detectable strand breaks. This is a commonly used approach (36,51,(110)(111)(112)(113)(114)(115)(116)(117) and we have demonstrated here that this is also an effective approach for MCL-5 lymphoblastoid cells using the CometChip (Figure 2D).…”

Section: Detecting Ber Activitymentioning

confidence: 67%

CometChip enables parallel analysis of multiple DNA repair activities

Ngo

Kaushal

et al. 2021

DNA Repair

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DNA damage can be cytotoxic and mutagenic, and it is directly linked to aging, cancer, and other diseases. To counteract the deleterious effects of DNA damage, cells have evolved highly conserved DNA repair pathways. Many commonly used DNA repair assays are relatively low throughput and are limited to analysis of one protein or one pathway. Here, we have explored the capacity of the CometChip platform for parallel analysis of multiple DNA repair activities. Taking advantage of the versatility of the traditional comet assay and leveraging micropatterning techniques, the CometChip platform offers increased throughput and sensitivity compared to the traditional comet assay. By exposing cells to DNA damaging agents that create substrates of Base Excision Repair, Nucleotide Excision Repair, and Non-Homologous End Joining, we show that the CometChip is an effective method for assessing repair deficiencies in all three pathways. With these applications of the CometChip platform, we expand the utility of the comet assay for precise, high-throughput, parallel analysis of multiple DNA repair activities.

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Influence of bariatric surgery induced weight loss on oxidative DNA damage

Cited by 14 publications

References 28 publications

Obese Adipose Tissue as a Driver of Breast Cancer Growth and Development: Update and Emerging Evidence

Obese Adipose Tissue as a Driver of Breast Cancer Growth and Development: Update and Emerging Evidence

Effect of cryopreservation on DNA damage and DNA repair activity in human blood samples in the comet assay

CometChip enables parallel analysis of multiple DNA repair activities

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