Influence of bcl-2-Related Proteins on Matrix Metalloproteinase Expression in a Rat Glioma Cell Line

The expression of core fucosylated E-cadherin in cancer cells and lung cancer patients: prognostic implications

“…Both MMP-2 and MMP-9 are two proteases most frequently expressed in cancer metastasis and angiogenesis as well as for cell migration or invasiveness [13]. By zymography, our data showed that the activity of MMP-2 and MMP-9 in RNAi/95D cells decreased compared to Mock/95D cells.…”

“…Gelatinase activity was assayed by zymography as described previously [13]. Mocked/95D cells and RNAi/95D cells grown at confluence were washed several times in PBS and then incubated with serum free medium for 48 h in the presence of DMSO or 1 µM herbimycin A.…”

The Tumor Necrosis Factor-like Weak Inducer of Apoptosis (TWEAK)-Fibroblast Growth Factor-inducible 14 (Fn14) Signaling System Regulates Glioma Cell Survival via NFκB Pathway Activation and BCL-XL/BCL-W Expression

“…Members of the BCL-2 gene family include the proapoptotic proteins BAD, BIK, and BID and the antiapoptotic proteins BCL-2, BCL-X L , and BCL-W (22). High expression levels of antiapoptotic BCL-2-related proteins have been found in many tumors, and up-regulation of BCL-2 and BCL-X L has been shown to be a key element in malignancy (23) and drug resistance (24,25). BCL-2 overexpression has been observed in several glioma cell lines and in glioma biopsies of various histological grade (26,27) and may confer resistance to radiotherapy and chemotherapy (28 -30).…”

Análise comparativa das distribuições espaciais de moléculas envolvidas na migração e invasão de um tumor cerebral de rato in vivo.

“…Esse fato sugere que a MMP-9 apresenta um papel mais importante na remodelagem angiogênica, enquanto a MMP-2 apresentaria um papel tanto na invasão quanto na angiogênese (BELLAIL et al, 2004;FORSYTH et al, 1999 (CHOE et al, 2002;BELLAIL et al, 2004;LAKKA et al, 2002a;LAKKA et al, 2002b;WICK et al, 2001;PARK et al, 2002;UHM et al, 1996). Oliver et al (2000) mostraram que proteínas da família do bcl-2 (como o bax e o bcl-xl) apresentou um papel na expressão de MMP-9. A família do bcl-2 apresenta propriedades tanto anti-apoptóticas (bcl-2 e bcl-xl), quanto pró-apoptóticas (bax) e a sua expressão foi alterada em gliomas.…”

“…Os pesquisadores observaram que linhagens de glioma transfectadas para expressarem bcl-2 e bcl-x apresentaram também uma menor expressão de MMP-9, e as células transfectadas com bax expressaram menos TIMP-1. Os autores ressaltaram que o controle do metabolismo migratório envolveu um balanço complexo entre MMPs e TIMPs e a expressão aumentada de TIMP-1 que, apesar de aparentemente paradoxical, também tem sido relatada em diversos tipos tumorais (OLIVER et al, 2000). condições hipóxias e/ou isquêmicas como derrames e processos infecciosos.…”