2020
DOI: 10.1021/acsomega.0c00334
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Influence of Binding Site Affinity Patterns on Binding of Multivalent Polymers

Abstract: Using inspiration from biology, we can leverage multivalent binding interactions to enhance weak, monovalent binding between molecules. While most previous studies have focused on multivalent binders with uniform binding sites, new synthetic polymers might find it desirable to have multiple binding moieties along the chain. Here, we probe how patterning of heterogeneous binding sites along a polymer chain controls the binding affinity of a polymer using a reactive Brownian dynamics scheme. Unlike monovalent bi… Show more

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Cited by 13 publications
(14 citation statements)
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“…Our results show the confined peptides are capable of specifically capturing β2m from the surface–liquid interface with micromolar affinity. This proof-of-concept work paves the way to the next challenge, which will be designing more complex devices in the form of coupled, or multivalent, binders [ 55 , 56 , 57 , 58 ] or antibody fragments [ 52 , 59 ] capable of further enhancing the binding affinity of the designed architectures.…”
Section: Discussionmentioning
confidence: 99%
“…Our results show the confined peptides are capable of specifically capturing β2m from the surface–liquid interface with micromolar affinity. This proof-of-concept work paves the way to the next challenge, which will be designing more complex devices in the form of coupled, or multivalent, binders [ 55 , 56 , 57 , 58 ] or antibody fragments [ 52 , 59 ] capable of further enhancing the binding affinity of the designed architectures.…”
Section: Discussionmentioning
confidence: 99%
“…The limited valence of lock-and-key type bonds creates competition for sites between bound and unbound target neighbors, reducing the influence of the polymer on the targets. This reduction in binding due to competition for high affinity sites is discussed further in our previous work on multivalent binding site patterns [ 34 ]. The promiscuous nature and high effective valency of non-specific attraction reduces competition and allows polymers to interact with more targets simultaneously.…”
Section: Resultsmentioning
confidence: 88%
“…Systems that have included multiple types of polymeric components did not explore the contributions of modulating non-specific and specific interactions between the two species [ 6 , 31 , 32 ]. Similarly, our previous research studied how the binding affinity changed between two species, a polymer and a colloid target, based on altering polymer characteristics (length, binding site patterns, and chain stiffness), but our previous studies did not explore the effects of combining specific and non-specific attraction to control the phase separation of the system [ 18 , 33 , 34 ].…”
Section: Introductionmentioning
confidence: 99%
“…The limited valence of lock-and-key type bonds creates competition for sites between bound and unbound target neighbors, reducing the influence of the polymer on the targets. This reduction in binding due to competition for high affinity sites is discussed further in our previous work on multivalent binding site patterns [39]. The promiscuous nature and high effective valency of non-specific attraction reduces competition and allows polymers to interact with more targets simultaneously.…”
Section: Non-specific Binding Interactionsmentioning
confidence: 81%