Influence of biotransformation on trophic transfer of the PAH, fluoranthene

Palmqvist, Annemette; Rasmussen, Lene Juel; Forbes, Valery E.

doi:10.1016/j.aquatox.2006.09.008

Cited by 29 publications

(11 citation statements)

References 36 publications

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“…As a consequence, sediment-bound PAHs can act as a reservoir of mutagenic hazards that favor the exposure of benthic biota [Barreira et al, 2007]. Moreover, their constant reintroduction to the water column via resuspension and trophic transfer [Meador et al, 1995;Palmqvist et al, 2006] could possibly affect the health of pelagic organisms by inducing adverse effects such as reproductive abnormalities [Kammann et al, 2004] and cancers [De Flora et al, 1991;Pinkney et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays

Aouadene¹,

Giorgio²,

Sarrazin³

et al. 2008

Environ and Mol Mutagen

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The present investigation evaluated the capacity of the Salmonella mutagenicity test, the comet assay, and the micronucleus assay to detect and characterize the genotoxic profile of river sediments. Three stations were selected on an urban river (Bouches du Rhône, France) exposed to various sources of industrial and urban pollution (StA, StB, and StC) and one station on its tributary (StD). One station in a nonurban river was included (REF). The concentrations of 16 polycyclic aromatic hydrocarbons (PAHs) were determined by HPLC, and the genotoxicity of the sediments was monitored by the Salmonella mutagenicity test (TA98 + S9, YG1041 +/- S9), the comet assay, and the micronucleus assay on CHO cells. Chemical analysis showed that the total PAH concentrations ranged from 23 microg kg(-1) dw (REF) to 1285 microg kg(-1) dw (StD). All the sediments were mutagenic in the Salmonella mutagenicity test. The mutagenicity was probably induced by the presence of nitroarenes (StA, StB, StC, and StD) and aromatic amines (REF) as deduced from the mutagenicity profiles of strains YG1041 +/- S9 and TA98 + S9. The comet assay revealed direct DNA lesions in REF, StA, and StB sediments and metabolization-dependent DNA damage in StC and StD. The micronucleus assay showed an absence of clastogenicity for StA +/- S9 and StC-S9, and a significant clastogenicity +/- S9 for the three other stations. The genotoxicity ranking determined by the comet assay + S9 matched the ranking of total and carcinogenic PAH concentrations, and this assay was found to be the most sensitive.

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Section: Introductionmentioning

confidence: 99%

Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays

Aouadene¹,

Giorgio²,

Sarrazin³

et al. 2008

Environ and Mol Mutagen

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“…Although the lower bioaccumulation factor of PYOH could be interpreted as reduced bioavailability, it most likely represents the enhanced ability for biotransformation of PYOH compared with PY. These data also provide further evidence that PAH metabolites can be as bioavailable from a food source as parent PAH, suggesting that trophic transfer of metabolites, as well as parent compounds, should be considered as an exposure route to PAH [12]. In the future, the bioavailability of these compounds could have to be incorporated into calculations of trophic transfer of PAH in more components of aquatic systems.…”

Section: Bioaccumulation and Biotransformationmentioning

confidence: 86%

“…As a result of these numerous sources, oxidized PAH have been detected in water and marine sediment [10,11]. PAH and metabolites that bioaccumulate in species low on the food chain can then be taken up by higher trophic levels through dietary exposure [12]. The tendency for such trophic transfer depends on numerous factors, including the chemical properties of the contaminant, the food source, and also the food source's residence time within and the pH of the gut of the organism [13].…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation and biotransformation of pyrene and 1‐hydroxypyrene by the marine whelk Buccinum undatum

Beach

Quilliam

Rouleau

et al. 2010

Enviro Toxic and Chemistry

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The fates of a phenolic contaminant and its hydrocarbon precursor have rarely been compared, especially in an invertebrate species. Two groups of Buccinum undatum were exposed to equimolar amounts of pyrene and 1‐hydroxypyrene over 15 d through their diets. Tissue extracts from the muscle and visceral mass were analyzed by liquid chromatography with fluorescence and mass spectrometry detection. Nine biotransformation products were detected in animals from both exposures. These included 1‐hydroxypyrene, pyrene‐1‐sulfate, pyrene‐1‐glucuronide, pyrene glucose sulfate, two isomers each of pyrenediol sulfate and pyrenediol disulfate, and one isomer of pyrenediol glucuronide sulfate. These compounds represent a more complex metabolic pathway for pyrene than is typically reported. Diconjugated metabolites were as important in animals exposed to pyrene as in those exposed to 1‐hydroxypyrene. Biotransformation products represented >90% of the material detected in the animals and highlight the importance of analyzing metabolites when assessing exposure. A mean of only 2 to 3% of the body burden was present in muscle compared with the visceral mass of both groups. The analytical methods were sufficiently sensitive to detect biotransformation products both in laboratory control whelks and in those sampled offshore. The tissue distribution of [14C]pyrene was also studied by autoradiography. Radioactivity was present primarily in the digestive and excretory system of the whelks and not in the gonads or muscle tissue. Environ. Toxicol. Chem. 2010;29:779–788. © 2009 SETAC

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“…4C for comparison] and up to ∼58% biotransformed in the study by Bach et al [10]), despite the fact that worms in the present study were exposed to both Flu and B a P (i.e., a total PAH exposure concentration of twice the concentrations used in both earlier studies). Recent experiments [28] in which Capitella sp. I were exposed to sediment concentrations of approximately 67 μg Flu/g dry weight sediment (331 nmol/g dry wt sediment) showed that these worms are able to keep a constant Flu biotransformation rate even at high Flu concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…I were exposed to sediment concentrations of approximately 67 μg Flu/g dry weight sediment (331 nmol/g dry wt sediment) showed that these worms are able to keep a constant Flu biotransformation rate even at high Flu concentrations. Palmqvist et al [28] found that worms biotransformed approximately 70% of the accumulated Flu equivalents within the 4 d of their experiment. After exposure to a total of 261 nmol PAH/g dry weight sediment for more than 1 d in the present study, the worms had biotransformed approximately 64% of the accumulated PAH equivalents (mean over exposure days from day 2 to the end of the exposure period).…”

Section: Discussionmentioning

confidence: 99%

Relative impact of coexposure compared to single‐substance exposure on the biotransformation and toxicity of benzo[a]pyrene and fluoranthene in the marine polychaete Capitella sp. I

Palmqvist

Rasmussen

Forbes

2008

Enviro Toxic and Chemistry

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Most biotransformation studies to date have concentrated on single-substance exposures. The biotransformation ability of organisms exposed to a single substance, however, may differ from that of organisms coexposed to binary or complex mixtures. The marine polychaete Capitella sp. I was exposed to either 31 microg/g dry weight sediment of benzo[a]pyrene (BaP) in a single-substance exposure or to a mixture of 31 and 28 microg/g dry weight sediment of BaP and fluoranthene (Flu), respectively (coexposure). Biotransformation capability and DNA damage were compared between treatments. Worms exposed to BaP alone accumulated total BaP equivalents to a significantly higher degree than coexposed worms (final body burden: 117.14 +/- 3.52 microg BaP equivalents/g dry wt tissue and 69.78 +/- 8.58 microg BaP equivalents/g dry wt tissue, respectively), probably because of a higher degree of BaP biotransformation in the latter. In addition, a larger percentage of the accumulated BaP equivalents was in the form of parent compound in worms exposed to BaP alone compared to coexposed worms on day 10 (87 and 56%, respectively). In coexposed worms, Flu was both accumulated and biotransformed to a considerably greater degree than BaP (final Flu body burden: 181.85 +/- 18.46 microg Flu equivalents/g dry wt tissue, 24% as parent compound). Neither treatment resulted in significant effects on growth or DNA damage. The present study shows that the extent to which bioaccumulation factors estimated from single-substance exposures have relevance for the field situation will depend on the degree to which accumulation is influenced by the presence of other contaminants. These results therefore are relevant for assessing risks from contaminated sediments.

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Influence of biotransformation on trophic transfer of the PAH, fluoranthene

Cited by 29 publications

References 36 publications

Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays

Evaluation of the genotoxicity of river sediments from industrialized and unaffected areas using a battery of short‐term bioassays

Bioaccumulation and biotransformation of pyrene and 1‐hydroxypyrene by the marine whelk Buccinum undatum

Relative impact of coexposure compared to single‐substance exposure on the biotransformation and toxicity of benzo[a]pyrene and fluoranthene in the marine polychaete Capitella sp. I

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