Influence of blood carbon dioxide pressure on growth index values in non-radiometric Bactec vials

Courcol, René; Durocher, A.; Robin, H; Martin, G.

doi:10.1007/bf01964055

Cited by 3 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… A high percentage of false‐positive vials (41 of 98 (41.8%)) originated from patients from the Bone Marrow Transplant Department. It has previously been shown [10] that abnormally high numbers of blood cells—mainly leukocytes—and hypercapnia can cause increased background CO 2 concentration and thus false positives. However, the leukocyte and thrombocyte counts of these specific patients both varied randomly from extremely low to very high with no apparent correlation (data not shown). False‐positive vials could actually be true positives, but of a kind which defies detection by standard laboratory techniques (Gram stain and subculture).…”

Section: Discussionmentioning

confidence: 99%

Comparison of BacT/Alert and BACTEC NR 860 blood culture systems in a laboratory not continuously staffed

Riest

Linde

Shah

1997

Clinical Microbiology and Infection

View full text Add to dashboard Cite

OBJECTIVE: To evaluate the performance of continuously working blood culture systems in a discontinuous laboratory system. METHODS: The systems used were BacT/Alert (Organon Teknika Corp., Durham, NC) and BACTEC NR 860 (Becton Dickinson Diagnostic Instruments, Sparks, Md) in a comparison in a laboratory staffed 8 1/2 h on Mondays to Fridays and 4 1/2 h on Saturdays. Blood culture bottles (BacT/Alert aerobic and anaerobic, BACTEC NR 26 A and NR 27 A) were received thrice daily. RESULTS: From 1824 pairs of blood culture vials, 110 clinically significant microorganisms were recovered by both BACTEC and BacT/Alert, 43 by BACTEC alone, and 33 by BacT/Alert alone. The differences between the systems in total recovery and in recovery of individual species were not statistically significant. The average detection times were 13.36 h for BACTEC and 13.93 h for BacT/Alert (P>0.1). These times represent only 35.6% (BACTEC) and 32.6% (BacT/Alert) of the total timespans from collection of blood to informing the ward of a positive result (tcrd, clinically relevant detection time). If 24 h per day blood culture processing conditions and continuous transport of vials to the laboratory had been available, these percentages would have risen to 87% (BACTEC) and 87.5% (BacT/Alert). Under such 'ideal' conditions, ttrd could have been reduced by 22.16 h using BACTEC and by 26.81 h using BacT/Alert. The BacT/Alert system showed more false-positive results than the BACTEC system (80 (4.39%) versus 23 (1.26%), P<0.001). CONCLUSIONS: No time benefit for detection of positive blood cultures is gained with continuously measuring systems, if loading and processing of vials is organized discontinuously, as in our laboratory.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of BacT/Alert and BACTEC NR 860 blood culture systems in a laboratory not continuously staffed

Riest

Linde

Shah

1997

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…When vials for the BACTEC NR660 were first employed, the GV and DR threshold settings used were fairly uniform; for example, threshold levels in a study of an anaerobic (NR7A) medium in 1985 to 1986 were as follows: GV = 30 and DR = 15 (3,6). The same values were reported as being recommended by the manufacturer in a study performed in 1988 (10).…”

Section: Discussionmentioning

confidence: 99%

“…Too low a threshold value will result in unproductive gram staining and a subculture which subsequently yields no organism (a false alarm). Such a result presumably arises from the presence of nonmicrobial carbon dioxide in the patient's blood or from the metabolic activity of blood cells (3). By contrast, setting the GV or DR thresholds too high will delay the recognition of vials that do contain organisms (a miss).…”

mentioning

confidence: 99%

Determination of growth value thresholds for BACTEC PLUS aerobic blood culture vials

McGowan

Metchock

1992

J Clin Microbiol

View full text Add to dashboard Cite

Growth value thresholds used to identify positive blood culture vials can be defined by users for each BACTEC NR-660 bacteremia detection instrument. Growth values were compared with the recovery of organisms from vials flagged as positive during the testing of 3.056 high-volume vials containing aerobic (BACTEC PLUS 26) medium over a 2-month period. Results showed that optimal threshold values for our use of these vials varied from those recommended by the manufacturer; if the thresholds defined from these data had been used during the study period, total vials flagged as positive from which no organisms were recovered (false alarms) would have been reduced from 181 (5.9/100 vials tested) to 71 (2.3/100 vials tested), with a minimal decrease in the identification of vials containing usual or occasional pathogens (hits). Adjustments of growth value thresholds by the individual user can make the use of BACTEC instruments more efficient by decreasing further processing of vials from which no organisms are recovered.on August 3, 2020 by guest

show abstract

Setting growth value thresholds for BACTEC vials: An approach to continuous quality improvement

McGowan

Metchock

Brooks

et al. 1993

Clinical Microbiology Newsletter

View full text Add to dashboard Cite

Influence of blood carbon dioxide pressure on growth index values in non-radiometric Bactec vials

Cited by 3 publications

References 9 publications

Comparison of BacT/Alert and BACTEC NR 860 blood culture systems in a laboratory not continuously staffed

Comparison of BacT/Alert and BACTEC NR 860 blood culture systems in a laboratory not continuously staffed

Determination of growth value thresholds for BACTEC PLUS aerobic blood culture vials

Setting growth value thresholds for BACTEC vials: An approach to continuous quality improvement

Contact Info

Product

Resources

About