Influence of blood contamination before or after surface treatment on adhesion of 4-META/MMA-TBB resin to root dentin

Abstract

The purpose of this study was to evaluate the influence of blood contamination before or after surface treatment on adhesion of 4-META/MMA-TBB resin. After bovine root dentin surfaces were contaminated with blood before or after dentin surface treatment with 10-3 solution, the contaminated surface was rinsed with water, air-dried, or re-treated with 10-3 solution. Dye leakage and microtensile bond strength (MTBS) of 4-META/MMA-TBB resin to dentin were measured after storage in water for 24 h. When blood contamination occurred before surface treatment, there was no significant difference in the leakage value and MTBS as compared with that of the uncontaminated group. When blood contamination occurred after surface treatment, the leakage value increased and MTBS significantly decreased (p<0.05) even if the blood was washed away. However, when the surface was re-treated with 10-3 solution after rinsing with water, the leakage value and MTBS were restored to those of the uncontaminated group.

Status of decontamination methods after using dentin adhesion inhibitors on indirect restorations: An integrative review of 19 publications

“…Takefu et al [ 26 ] (B#1) reported that the leakage value and micro-tensile bond strength were restored to an uncontaminated group after re-treating the surface with 10-3 solution (10% citric acid [pH 0.86] and 3% ferric chloride) and rinsing with water; however, re-etching by phosphoric acid after saliva contamination reduced the bond strength, as mentioned in a previous section ( 3.2 . Saliva contamination).…”

“…A total of 52 papers were identified (from 1990 to 2020), 39 of which were excluded after a review (about adhesives, not using resin cement: 20 papers; not including bond strength test: 4 papers; review without any data: 3 papers; no examination of decontamination or simple explanation of the contamination effects of decreased bond strength: 10 papers; and using glass ionomer cement: 2 papers). We also included six papers found in a manual search, resulting in a total of 19 articles for inclusion in this review [ [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] , [19] , [20] , [21] , [22] , [23] , [24] , [25] , [26] , [27] , [28] , [29] , [30] ] ( Fig. 2 ).…”

Evaluation of Restorative Techniques for Vertically Fractured Roots

“…This may be because of effective and sufficient resin-impregnated layer formations by UB applications on bonding durability and stability [ 22 , 23 ]. Furthermore, the tooth surface treatments using 10-3 solutions and EDTA were more effective compared with phosphate solution in removing contaminants and thin layers of cutting debris (smear layers) without excess dentin decalcification [ 24 , 25 , 26 ], which may have improved the bond strength. When performing adhesive restoration methods for vertically fractured roots followed by intentional replantation in a clinical setting, blood contamination for the fractured root surface is assumed.…”

“…When performing adhesive restoration methods for vertically fractured roots followed by intentional replantation in a clinical setting, blood contamination for the fractured root surface is assumed. However, a previous study reported that the µTBS of the contaminated dentin after treatment with 10-3 solution was equivalent to that of noncontaminated dentin [ 24 ]. Therefore, using 10-3 solution may be beneficial in adhesive restoration methods.…”

Treatment of saliva contamination of resin core foundation before adhesive luting

“…During fabrication and trial fitting of prosthetic restorations, abutment surfaces, such as dentin, restorative materials, and core foundation materials, may be contaminated by saliva and blood 3,4) . Therefore, adherent surfaces should be thoroughly cleaned and conditioned before cementation with resin luting cement to ensure long-term bonding stability 5) .…”