Influence of Blood Flow on Adsorption of β₂‐Microglobulin onto AN69 Membrane

Abstract

Adsorption onto the dialyzer membrane is a contributing factor to the elimination of P,-microglobulin (P,M) from the sera of uremic patients. The purpose of this prospective study was to ascertain the influence of the blood flow rate on adsorption of P2M onto the polyacrylonitrile (AN69) hollow-fiber dialyzer membrane in 8 patients during regular hemodialysis (HD). Blood first passed through a low-flux polysulfone dialyzer and then through an AN69 dialyzer, which was not in contact with the dialysis fluid. During the investigation period (first hour of the HD session), the blood flow rate was 100 mli min (first part of the study), 200 mumin (second part of the study), and 300 ml/min (third part of the study). Ultrafiltration was not performed during the investigation period. At the start of the HD sessions, the serum concentration of P,M in the afferent blood line did not differ significantly among the 3 parts of the study. Serum P,M was measured in samples taken from the afferent and efferent blood lines of the AN69 dialyzer at 5, 10, 15, 30, 45, and 60 min. The serum P2M concentration decreased significantly in blood that had passed through the AN69 dialyzer. This decrease, indicating membrane adsorption, was maximal during the first part and minimal during the third part of study. The decrease in the contact time between the blood and the AN69 could be the underlying cause. The calculated quantities of P2M adsorbed onto the AN69 membrane (44.2 * 10.2,43.2 12.1, and 42.6 17.3 mg) did not differ significantly among the 3 parts of the study. These results suggest that an increase in blood flow rate from 100 to 300 mllmin did not significantly affect the quantity of P,M adsorbed onto the AN69 membrane.

A Serial Copolymerization Procedure for Manufacturing Immunoadsorption Walls as a Potential Unit in Conjunction With Hemodialysis Filters

“…The present or related studies have suggested that saturation of an immunoadsorption process could be completed within a relatively short period of time (16). On the other hand, the removal of β‐2M is primarily dependent on the available adsorption surface but is independent of the blood flow rate (34,35). Thus, for a blood flow‐circuit as shown previously (14), the blood flow rate could be tentatively maintained as that in the hemodialyzer but immunoadsorption procedure could be performed in less time than hemodialysis.…”

Creatinine adsorption capacity of electrospun polyacrylonitrile (PAN)‐zeolite nanofiber membranes for potential artificial kidney applications

“…More than 70% of 615,000 end stage renal disease patients are on dialysis in America. 1 Although several types of hollow fiber dialysis membranes which eliminating uremic toxins though diffusion process are used in clinics, [2][3][4][5] the dialysis process is still inconvenient, time consuming, and expensive.…”