Influence of blue light on Streptococcus mutans re-organization in biofilm

Chebath-Taub, Daniella; Steinberg, Doron; Featherstone, J.D.B.; Feuerstein, Osnat

doi:10.1016/j.jphotobiol.2012.08.004

Cited by 46 publications

(45 citation statements)

References 23 publications

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“…Recently, PDT has been used as an antimicrobial therapy with some photosensitizers to target a variety of microorganisms, such as P. gingivalis, Fusobacterium nucleatum, Propionibacterium acnes, S. aureus, S. mutans, and E. coli [21][22][23][24][25][26]. Guffey and Wilborn reported that 405 nm blue light had a bactericidal effect on two aerobes, Staphylococcus aureus and Pseudomonas aeruginosa [25,26]. In our previous study, we investigated the most effective wavelength for inhibiting P. gingivalis growth and confirmed that 400-410 nm blue light was the most effective for inhibiting this bacterium even without a photosensitizer [27].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Imamura

Tatehara

Takebe

et al. 2014

International Journal of Photoenergy

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The purpose of this study is to evaluate the usefulness of 405 nm monochromatic laser irradiation as an alternative management for prevention and/or treatment of endodontic infections. A monochromatic laser-emitting device equipped with a 405-nm laser diode was developed. Using this device, the effect of 405 nm laser irradiation on the growth of Porphyromonas gingivalis, Prevotella intermedia, Enterococcus faecalis, and Candida albicans, which are microorganisms associated with persistent endodontic infections, was evaluated by viable colony counting. As a result, the irradiation with a 405 nm laser had a significant bactericidal/fungicidal effect on P. gingivalis, P. intermedia, and C. albicans, whereas the growth of E. faecalis was not affected by the irradiation. The inhibition rate in P. gingivalis and P. intermedia was ∼60% and ∼80%, respectively, following irradiation at 0.2 W for 300 sec. The inhibition rate in C. albicans was ∼90% following irradiation at 0.2 W for 1200 sec. These results indicate that 405 nm monochromatic laser irradiation exerts a bactericidal/fungicidal effect on these microorganisms. The present study clearly demonstrates that 405 nm laser irradiation is a promising alternative management strategy for prevention and/or treatment of endodontic infections.

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Section: Introductionmentioning

confidence: 99%

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Imamura

Tatehara

Takebe

et al. 2014

International Journal of Photoenergy

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“…The combination of a specific photosensitizer with a light source of appropriate wavelength, availability of oxygen and also the type of a particular organism or a group of microorganisms, plays a vital role in the application of photodynamic therapy [14,18,37]. The mechanism behind photoinactivation of S. mutans is not known, and to our knowledge only one study has used Violet-blue light with no photosensitizer on S. mutans biofilms [6]. They used a plasma arc lamp with 400 to 500 nm wavelength and a power density of 1.14 W/cm 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Phototherapy without exogenous photosensitizers was used to eliminate Porphyromonas gingivalis, Prevotella intermedia, Prevetolla nigrescens and Prevetolla melaninogenica and it is believed that endogenous porphyrins in the oral black-pigmented periodontal bacteria are excited at 380 to 520 nm releasing reactive oxygen species (ROS) [37]. Recent studies by Chebath-Taub et al [6] and Steinberg et al [38] indicated that S. mutans biofilm loses the ability to form new biofilm when exposed to blue light in the range of 400 to 500 nm and proposed a new concept of delayed antibacterial activity. In the present study, we hypothesized that violet-blue light specifically from a QLF device with an exposure time of 5 min has the ability to kill S. mutans or inactivate established S. mutans biofilm formed during 12 to 16 h of growth without any exogenous photosensitizer.…”

Section: Introductionmentioning

confidence: 99%

Photo Inactivation of Streptococcus mutans Biofilm by Violet-Blue light

et al. 2016

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Among various preventive approaches, non-invasive phototherapy/photodynamic therapy is one method used to control oral biofilm. Studies indicate that light at specific wavelengths has a potent antibacterial effect. The objective of this study was to determine the effectiveness of violet-blue light at 380-440 nm to inhibit biofilm formation of Streptococcus mutans or kill S. mutans. S. mutans UA159 biofilm cells were grown for 12-16 h in 96-well flat-bottom microtiter plates using Tryptic Soy broth (TSB) or TSB with 1% sucrose (TSBS). Biofilm was irradiated with violet-blue light for 5 min. After exposure, plates were re-incubated at 37°C for either 2 or 6 h to allow the bacteria to recover. A crystal violet biofilm assay was used to determine relative densities of the biofilm cells grown in TSB, but not in TSBS, exposed to violet-blue light. The results indicated a statistically significant (p < 0.05) decrease compared to the nontreated groups after the 2 or 6h recovery period. Growth rates of planktonic and biofilm cells indicated a significant reduction in the growth rate of the violet-blue light-treated groups grown in TSB and TSBS. Biofilm viability assays confirmed a statistically significant difference between Violet-blue light-treated and non-treated groups in TSB and TSBS. Visible Violet-blue light of the electromagnetic spectrum has the ability to inhibit S. mutans growth and reduce the formation of S. mutans biofilm. This in-vitro study demonstrated that Violet-blue light has the capacity to inhibit S. mutans biofilm formation. Potential clinical applications of light therapy in the future remain bright in preventing the development and progression of dental caries.

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“…The synergistic effect of 20-s blue light treatment with hydrogen peroxide reduced the bacterial growth up to 96% (11). Whereas a delayed antibacterial effect (12,13) was reported by Feuerstein et al (11) in an S. mutans biofilm grown for 6 h, whereas Chebath et al (14) observed increased antibacterial effect after 3-, 5-, 7-, and 10-min treatment (13). Photoinactivation of S. mutans biofilm has been achieved through a 5-min treatment with violet-blue light with a peak wavelength of 405 nm (15).…”

Section: Introductionmentioning

confidence: 96%

Effect of phototherapy on the metabolism of <i>Streptococcus mutans</i> biofilm based on a colorimetric tetrazolium assay

Gomez

Huang

Eckert

et al. 2018

Journal of Oral Science

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The aim of this in vitro study was to determine the effect of violet-blue light on the metabolic activity of early Streptococcus mutans biofilm, reincubated at 0, 2, and 6 h after 5 min of violet-blue light treatment. S. mutans UA159 biofilm cells were cultured for 12 to 16 h in microtiter plates with Tryptic Soy broth (TSB) or TSB with 1% sucrose (TSBS) and irradiated with violet-blue light for 5 min. After irradiation, the plates were reincubated at 37°C for 0, 2, or 6 h in 5% CO 2 . Colorimetric tetrazolium salt reduction assay was used to investigate bacterial metabolic activity. Mixed model ANOVA was used to find the difference between the violet-blue light treated and nontreated groups. Bacterial metabolic activity was significantly lower in the violet-blue light group for TSB than in the nontreated group (P < 0.0001) regardless of recovery time. However, the differences between metabolic activity in the treated groups without sucrose decreased over time. For TSBS, metabolic activity was significantly lower with violet-blue light at 0 and 2 h. Violet-blue light inhibited the metabolic activity of S. mutans biofilm cells in the light-treated group. This finding may present a unique treatment method for patients with active caries.

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Influence of blue light on Streptococcus mutans re-organization in biofilm

Cited by 46 publications

References 23 publications

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Antibacterial and Antifungal Effect of 405 nm Monochromatic Laser on Endodontopathogenic Microorganisms

Photo Inactivation of Streptococcus mutans Biofilm by Violet-Blue light

Effect of phototherapy on the metabolism of <i>Streptococcus mutans</i> biofilm based on a colorimetric tetrazolium assay

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