Influence of Blue Light on the Leaf Morphoanatomy of<i>In Vitro Kalanchoe pinnata</i>(Lamarck) Persoon (Crassulaceae)

Leal-Costa, Marcos Vinícius; Nascimento, Luana Beatriz dos Santos; Moreira, Nattacha dos Santos; Reinert, Fernanda; Costa, Sônia Soares; Lage, Celso Luiz Salgueiro; Tavares, Eliana Schwartz

doi:10.1017/s1431927610000279

Cited by 12 publications

(9 citation statements)

References 36 publications

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“…The results of the present study also showed that, compared with monochromatic red light, the accumulation of 3,4-dihydroxybenzoic acid, gallic acid, and ferulic acid in P. cynaroides plantlets was enhanced by the presence of blue light, whether individually or in combination with red light (red + blue LEDs) ( Table 2). This is in agreement with studies that showed that blue light stimulates the biosynthesis of phenolic compounds in plants (Leal-Costa et al, 2010;Meng et al, 2004;Rao and Ravishankar, 2002;Saleh, 2007). Similarly, chlorogenic acid in lettuce seedlings grown under blue-containing LEDs was also higher than those irradiated by red lights (Johkan et al, 2010).…”

Section: Resultssupporting

confidence: 92%

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Wu¹,

Lin²

2012

horts

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The effects of light quality emitted by light-emitting diodes (LEDs) on the growth and morphogenesis, and concentrations of endogenous phenolic compounds of Protea cynaroides L. plantlets in vitro, were investigated. Plantlets were cultured under four light treatments: conventional fluorescent lamps (control), red LEDs (630 nm), blue LEDs (460 nm), and red + blue LEDs (1:1 photosynthetic photon flux). Four phenolic compounds extracted from the plantlets were analyzed: 3,4-dihydroxybenzoic acid, gallic acid, caffeic acid, and ferulic acid. The highest rooting percentage was observed in plantlets cultured under red LEDs (67%) compared with 7% under conventional white fluorescent light, 13% under blue LEDs, and 13% under red + blue LEDs. The highest number of roots per plantlet was also found under red LEDs, whereas a significantly lower number of roots per plantlet was obtained under the other light treatments. Furthermore, red light promoted the formation of new leaves in P. cynaroides plantlets. However, the highest leaf dry weight (53.8 mg per plantlet) was found in plantlets irradiated by the combination of red and blue LEDs. Phenolic analyses showed that the lowest concentrations of 3,4-dihydroxybenzoic acid (4.3 mg·g−1), gallic acid (7.0 mg·g−1), and ferulic acid (7.4 mg·g−1) were detected in plantlets exposed to red light, whereas those irradiated by white fluorescent light contained the highest concentration. A significant inverse correlation (r = –0.419) was established between 3,4-dihydroxybenzoic acid and rooting percentage. Strong inverse correlations were also established between 3,4-dihydroxybenzoic acid and number of roots per plantlet (r = –0.768) as well as between ferulic acid and number of roots per plantlet (r = –0.732). These results indicate that the stimulation of root formation in P. cynaroides plantlets under red LEDs is the result of the low endogenous concentrations of 3,4-dihydroxybenzoic acid and ferulic acid.

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Section: Resultssupporting

confidence: 92%

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Wu¹,

Lin²

2012

horts

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“…In a previous study, the production of phenolic compounds increased in K. pinnata grown in vitro under supplemental blue light (Leal-Costa et al, 2010).…”

Section: Microchemical Testsmentioning

confidence: 78%

“…Some anatomical studies have examined K. pinnata (Jain et al 2008;Anjoo & Kumar, 2010;Leal-Costa et al 2010) and K. crenata (Hyakutake & Grotta, 1972). However, these studies provided neither information about the influence of environmental light intensity on the leaf anatomy of these species, nor a detailed anatomical description of them.…”

Section: Introductionmentioning

confidence: 99%

Comparative anatomy of leaves of Kalanchoe pinnata and K. crenata in sun and shade conditions, as a support for their identification

Moreira¹,

Nascimento²,

Leal-Costa³

et al. 2012

Rev. bras. farmacogn.

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Kalanchoe pinnata (Lam.) Pers. and K. crenata (Andrews) Haw., Crassulaceae, are popularly used in the treatment of many diseases. Their biological activities, such as anti-leishmaniasis and analgesic, can be useful in phytotherapy. Both species are often misidentified as the other, because of their similar popular uses and names, and the similar external morphology of the leaves. We investigated the existence of anatomical characters that will permit correct identification of the species grown in shade and in sun conditions. We also contribute with new observations on the leaf anatomy of K. pinnata and K. crenata. Fixed (FAA 70 ) leaves were used, and their sections were embedded in Leica historesin. Hydathodes were observed in both species, and for the first time were anatomically described in K. crenata. The species showed anatomical differences in relation to the presence of epidermal idioblasts only in K. crenata, the different pattern of distribution of subepidermal idioblasts, and the presence of leaf buds only in K. pinnata.

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“…Previous studies showed the presence of phenolic-producing cells located in the mesophyll of Inula viscosa (L.) Aiton leaves (Nikolakaki and Christodoulakis 2004) as well as Crassula multicava Lem. (Castro and Demarco 2008) and several Kalanchoë species (Leal-Costa et al 2010;Moreira et al 2012;Chernetskyy et al 2018). Different classes of phenolic compounds serve different functions.…”

Section: Discussionmentioning

confidence: 99%

Sites of secretion of bioactive compounds in leaves of Dracocephalum moldavica L.: anatomical, histochemical, and essential oil study

et al. 2019

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Dracocephalum moldavica L. is an aromatic plant emitting intense lemon scent. The aboveground parts of the plants constitute raw material for medicine and food industry. In contrast to the comprehensively investigated trichomes, there are only few studies of the histochemical characteristics of the leaves of essential oil-bearing plants from the family Lamiaceae. The present study shows the micromorphology, anatomy, and histochemistry of the leaves of the analysed species. The research aimed to determine the location of essential oil and other specialised metabolites in leaf tissues. The investigations of fresh and fixed material were carried out with the use of light, fluorescence, and scanning electron microscopy. Additionally, the content and composition of essential oil in the leaves were determined with the GC/MS method. The leaf epidermis had non-glandular unbranched trichomes and three types of glandular trichomes: peltate as well as long and short capitate trichomes. The results of the histochemical assays showed positive reactions to lipids and to some secondary compounds such as essential oil, terpenes, phenolic compounds, and flavonoids in all types of the glandular and non-glandular trichomes. The same compounds were found in the epidermis cells of the leaves. The results of the present study indicate that the intense smell of the leaves is associated with emission of essential oil not only by the glandular and non-glandular trichomes but also by the leaf epidermis cells. The main components in the essential oil (0.10%) include geranial, neral, geraniol, nerol, and trans-myrtanol acetate. Since D. moldavica has been used as an adulterant of Melissa officinalis L., the anatomical traits of leaves and the essential oil composition in both species were compared in the study.

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Influence of Blue Light on the Leaf Morphoanatomy ofIn Vitro Kalanchoe pinnata(Lamarck) Persoon (Crassulaceae)

Cited by 12 publications

References 36 publications

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Red Light-emitting Diode Light Irradiation Improves Root and Leaf Formation in Difficult-to-propagate Protea cynaroides L. Plantlets In Vitro

Comparative anatomy of leaves of Kalanchoe pinnata and K. crenata in sun and shade conditions, as a support for their identification

Sites of secretion of bioactive compounds in leaves of Dracocephalum moldavica L.: anatomical, histochemical, and essential oil study

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