Influence of Brain and Azadirachtin on Trypanosoma cruzi Development in the Vector, Rhodnius prolixus

Gonzalez, Marcelo S.; Nogueira, Nadir F. S.; Mello, C.B.; Souza, Wanderley de; Schaub, Günter A.; Azambuja, Patrı́cia; Garcia, Elói S.

doi:10.1006/expr.1998.4387

Cited by 67 publications

(52 citation statements)

References 24 publications

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“…Recently, Gonzalez et al (1999) explored the techniques of decapitation and head transplantation on the development of T. cruzi in R. prolixus. They demonstrated that decapitation as well as azadirachtin treatment induce a diminishing of the parasite infection.…”

Section: Trypanosoma Cruzi and Triatomine Neu-roendocrine Interactionmentioning

confidence: 99%

“…Thus, we developed in our laboratory the Rhodnius prolixus model to study several parameters related to the high degree of interaction between T. cruzi and invertebrate vector. Basically, this model implies in feeding of larvae and adults of R. prolixus, through a special membrane feeding apparatus, on blood containing different strains/clones of T. cruzi and, at different intervals, determination of the number of parasite in the gut, urine and feces of the vector (see Garcia & Azambuja 1991, Gonzalez et al 1998a, 1999. Herein, we will describe experiments, using this useful parasite-vector model, to elucidate some mechanisms involved in the interaction T. cruzi-triatomine insect and also link insect factors with the success or the failure of strains/clones of parasites to establish the infection in the digestive tract of the vector.…”

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confidence: 99%

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Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

Garcia

Gonzalez

1999

Mem. Inst. Oswaldo Cruz

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Section: Trypanosoma Cruzi and Triatomine Neu-roendocrine Interactionmentioning

confidence: 99%

mentioning

confidence: 99%

Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

Garcia

Gonzalez

1999

Mem. Inst. Oswaldo Cruz

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“…Rhodnius prolixus, an important Chagas' disease vector in America, is traditionally used as a model for studies of insect physiology (Wigglesworth, 1934a(Wigglesworth, ,b, 1943Garcia et al, 1990), control vector strategies (Garcia;Azambuja, 2004) and vector parasite interactions (Chagas, 1909;Dias, 1943;Garcia;Azambuja, 1991;Garcia et al, 1999;Gonzalez et al, 1999Gonzalez et al, , 2000Kollien;Schaub, 2000;Cortez et al, 2002;Kelecom et al, 2002a). In this work, studies were carried out with the essential oil obtained from Pilocarpus spicatus plants (popularly know as "Jaborandi da restinga") -collected in natura in the state of Rio de Janeiro (Brazil) -looking for effects produced on blood ingestion, mortality, molt and movement of fi fth-instar nynphae of R. prolixus.…”

Section: Introductionmentioning

confidence: 99%

Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilaire (Rutaceae) on the development of Rhodnius prolixus nymphae

Mello¹,

Uzeda²,

Bernardino³

et al. 2007

Rev. bras. farmacogn.

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RESUMO: "Efeitos do óleo essencial de Pilocarpus spicatus Saint-Hilaire (Rutaceae) no desenvolvimento de ninfas de Rhodnius prolixus". Bioensaios contra ninfas de 5º estádio de Rhodnius prolixus foram conduzidos utilizando-se óleo essencial de Pilocarpus spicatus extraído por hidrodestilação. Os principais resultados podem ser resumidos como se segue: (i) altos níveis de toxicidade e paralisia associados à discreta inibição da muda foram induzidos pela aplicação tópica de 0,5 μL ou 1,0 μL do óleo essencial por inseto; (ii) fagoinibição parcial, altos níveis de inibição da muda, período intermuda prolongado e alto número de insetos paralisados mas ausência de toxicidade foram observados após tratamento oral com 5,0 μL ou 10 μL de óleo essencial de P. spicatus por mL de sangue ingerido. A importância destes resultados em relação a eventos biológicos relevantes em R. prolixus é aqui discutida. Unitermos: Rhodnius prolixus, Pilocarpus spicatus, óleo essencial.ABSTRACT: Bioassays against fi fth-instar nymphae of Rhodnius prolixus were conducted with essential oil of Pilocarpus spicatus extracted by hydrodistillation. The main results may be summarized as follows: (i) high levels of toxicity and paralysis together with discrete moulting inhibition were caused by topical application of either 0.5 μL or 1.0 μL per insect of the crude essential oil; (ii) partial fagoinhibition, high moulting inhibition, prolonged intermoulting period and high number of paralyzed insects, but no toxicity were observed after oral treatment using either 5 μL or 10 μL of Pilocarpus spilcatus essential oil per mL of ingested blood meal. The importance of these results in relation to the relevant biological events in R. prolixus is herein discussed.

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“…Finally, once at the rectum, epimastigotes weakly attach to the hindgut cuticle and transform into metacyclic trypomastigotes, which are expelled with triatomine urine and feces and are then able to infect mammalian hosts [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

“…The attachment of T. cruzi epimastigotes to the PMM has been suggested to be important for parasite division [9]. Recently, the involvement of glycoinositolphospholipids (GIPLs), the major surface glycoconjugates of T. cruzi epimastigotes, in the adhesion of the parasite to the posterior midgut was demonstrated [14].…”

Section: Introductionmentioning

confidence: 99%

Cell Signaling During Trypanosoma cruzi Development in Triatominae

Silva-Neto¹

2010

TOPARAJ

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Abstract:The year 2009 is the centennial anniversary of the original description of Chagas disease by Carlos Chagas. During the last 100 years, several advances have occurred regarding our knowledge of the development of Trypanosoma cruzi and its travel along the gut of Triatominae vectors. We have also witnessed the completion of both the human and parasite genome projects; the genome of one of Chagas disease vectors, Rhodnius prolixus, is currently being sequenced. The development of T. cruzi in triatomine gut relies on several biochemical and molecular processes. The biochemistry of blood digestion and the molecular and biological aspects of parasite development are well known. However, several signaling molecules are generated during blood digestion, and their effects on parasite biology are only beginning to be understood. Here, we will summarize our current knowledge in this area with an emphasis on heme and bioactive lipids. In addition, we will highlight some recently described members of the parasite signaling machinery, which were identified through high-throughput studies, but whose ligands are unknown thus far. Finally, we will discuss some potential aspects for future investigation in this area that may strengthen our view of such a concomitant biological process in the next years.

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Influence of Brain and Azadirachtin on Trypanosoma cruzi Development in the Vector, Rhodnius prolixus

Cited by 67 publications

References 24 publications

Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

Effects of the essential oil obtained from Pilocarpus spicatus Saint-Hilaire (Rutaceae) on the development of Rhodnius prolixus nymphae

Cell Signaling During Trypanosoma cruzi Development in Triatominae

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