Influence of brief immersion in an aqueous solution of sodium chloride and/or copper sulphate on pikeperch (Sander lucioperca (L.)) blood parameters

Abstract

Research focusing on the effects of chemotherapeutics on the physiological state of fish determines their application in aquaculture. The aim of the study was to determine the impact of sodium chloride (NaCl) and/or copper sulphate (CuSO4) on the hematological and biochemical parameters of the blood plasma of pikeperch, Sander lucioperca, (body weight − 346.42 ± 44.82 g; body length − 29.8 ± 1.3 cm) during rearing in recirculating aquaculture systems (RAS). Three groups of fish were subjected to treatment with NaCl (5 g/L), CuSO4 (1.5 mg/L), and NaCl+CuSO4 (5 g/L + 1.5 mg/L). Blood was drawn 0, 24 and 48 h following immersion. Significant differences were noted immediately following the conclusion of immersion: mean corpuscular volume (MCV; elevated values in the NaCl+CuSO4 group) and mean corpuscular hemoglobin concentration (MCHC; elevated values in the NaCl group). Immediately following fish exposure in all three experimental groups (0 h), significantly elevated glucose and lactic acid levels were recorded as were lowered levels of chloride ions (Cl−). After 24 and 48 h, significant differences were observed in Cl−. In the NaCl group lowered levels of this parameter were sustained after 48 h, while in the other two groups this was sustained for 24 h. It was confirmed that the physiological impact on the pikeperch of the prophylactic bath in sodium chloride and/or copper sulphate was short and that the treatments did not have a significant impact on the health condition of pikeperch.

Influence of brief immersion in an aqueous solution of sodium chloride and/or copper sulphate on the physiological state of pikeperch (Sander lucioperca (L.)) selects

“…Elevated activity of this enzymes was only observed immediately following the conclusion of immersion in the CuSO 4 group. Similar observations were reported in other studies when juvenile pikeperch specimens were exposed to analogous immersion conditions (NaCl 5 g l -1 , CuSO 4 1.5 mg l -1 ) at a higher water temperature (22°C) (Ro¿yñski et al 2022). Fluctuations in AST values could also signal liver dysfunction.…”

“…in the variant in which NaCl and CuSO 4 were applied together. Elevated glucose levels were also noted in other studies of juvenile pikeperch immersed in sodium chloride and copper sulphate solutions identical to those applied in the present study (NaCl 5 g l -1 , CuSO 4 1.5 mg l -1 ) but at a different water temperature (22°C) (Ro¿yñski et al 2022). Similar reactions were also noted in juvenile pikeperch immersed in sodium chloride solutions of various concentrations (BW -94 g; NaCl concentrations of 10-20 g l -1 ), and elevated glucose values were noted in these fish up to 24 h after the conclusion of immersion (Demska-Zakêoe et al 2021).…”

The Combined Use of Copper Sulfate and Trichlorfon Exerts Stronger Toxicity on the Liver of Zebrafish

“…For example, 4 mg/L of (1/10 dose of LC50) copper sulfate was confirmed to have harmful effects on the fertility of male Nile tilapia [ 10 ], while 0.4 mg/L of Cu 2+ induced genotoxicity and hepatic impairments in spotted snakehead fish [ 11 ]. Meanwhile, studies have demonstrated that a prophylactic bath treatment in copper sulphate has short physical impacts on pikeperch, and the treatment does not significantly impact the health condition of pikeperch [ 12 ]. The antioxidant system plays a crucial role in combating oxidative stress and maintaining the redox balance within cells.…”