Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by
            <i>Pleurotus ostreatus</i>
            in Soil

Baldrián, Petr; Wiesche, Carsten in der; Gabriel, Jiřı́; Nerud, F.; Zadražil, František

doi:10.1128/aem.66.6.2471-2478.2000

Cited by 199 publications

(118 citation statements)

References 63 publications

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“…Activities of peroxidases, including Mnperoxidase were assayed using 3,3-dimethylaminobenzoic acid and 3-methyl-2-benzothiazolinone hydrazone in succinate/lactate buffer (100 mM, pH 4?5), as described previously (Ngo & Lenhoff, 1980;Bourbonnais & Paice, 1990;Baldrian et al, 2000). All spectrophotometric measurements were done in a microplate reader (Sunrise, Tecan) or a UV-VIS spectrophotometer (Lambda 11, Perkin-Elmer).…”

Section: Methodsmentioning

confidence: 99%

Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus – production of extracellular enzymes and characterization of the major cellulases

2006

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Piptoporus betulinus is a common wood-rotting fungus parasitic for birch (Betula species). It is able to cause fast mass loss of birch wood or other lignocellulose substrates. When grown on wheat straw, P. betulinus caused 65 % loss of dry mass within 98 days, and it produced endo-1,4-b-glucanase (EG), endo-1,4-b-xylanase, endo-1,4-b-mannanase, 1,4-b-glucosidase (BG), 1,4-b-xylosidase, 1,4-b-mannosidase and cellobiohydrolase activities. The fungus was not able to efficiently degrade crystalline cellulose. The major glycosyl hydrolases, endoglucanase EG1 and b-glucosidase BG1, were purified. EG1 was a protein of 62 kDa with a pI of 2?6-2?8. It cleaved cellulose internally, produced cellobiose and glucose from cellulose and cellooligosaccharides, and also showed b-xylosidase and endoxylanase activities. The K m for carboxymethylcellulose was 3?5 g l "1 , with the highest activity at pH 3?5 and 70 6C. BG1 was a protein of 36 kDa with a pI around 2?6. It was able to produce glucose from cellobiose and cellooligosaccharides, but also produced galactose, mannose and xylose from the respective oligosaccharides and showed some cellobiohydrolase activity. The K m for p-nitrophenyl-1,4-b-glucoside was 1?8 mM, with the highest activity at pH 4 and 60 6C, and the enzyme was competitively inhibited by glucose (K i =5?8 mM). The fungus produced mainly b-glucosidase and b-mannosidase activity in its fruit bodies, while higher activities of endoglucanase, endoxylanase and b-xylosidase were found in fungus-colonized wood.

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Section: Methodsmentioning

confidence: 99%

Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus – production of extracellular enzymes and characterization of the major cellulases

2006

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“…The weight loss of straw incubated with P. chrysosporium was optimum at Pb concentrations of 30 mg·kg −1 , but proceeded, too, at 400 mg·kg −1 [117]. The colonization of non-sterile soil by the basidiomycete Pleurotus ostreatus reached limits at concentrations of 50-100 mg·kg −1 DW in Hg and 100-500 mg·kg −1 in Cd [113]. An A-type soil lot of 41 mg·kg −1 ·DW in Cd, 156 mg·kg −1 in Pb, and 3320 mg·kg −1 in Zn enabled normal mycelial growth and fructification by the lignicolous basidiomycetes Hypholoma fasciculare, Kuehneromyces mutabilis, and P. ostreatus [118].…”

Section: Applicability Of the Herbage From The Metalliferous Soil Amentioning

confidence: 99%

“…However, the fungi used in the preparatory delignification of the biomass feedstock for bioethanol production face the full toxicity of internal HM. The resulting oxidative stress leads to the damage of cellular biomolecules such as DNA, lipids, and proteins by reactive oxygen species and may result in cell death [110][111][112] and restrictions in mycelial growth, enzymatic activity, and the rate of substrate conversion [113][114][115]. Toxicity effects of Cd as pronounced in liquid media drastically diminish in the presence of soils, organic matter, and high concentrations of dissolved salts [116].…”

Section: Applicability Of the Herbage From The Metalliferous Soil Amentioning

confidence: 99%

Gradual Accumulation of Heavy Metals in an Industrial Wheat Crop from Uranium Mine Soil and the Potential Use of the Herbage

Gramss

Voigt

2016

Agriculture

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Abstract:Testing the quality of heavy-metal (HM) excluder plants from non-remediable metalliferous soils could help to meet the growing demands for food, forage, and industrial crops. Field cultures of the winter wheat cv. JB Asano were therefore established on re-cultivated uranium mine soil (A) and the adjacent non-contaminated soil (C). Twenty elements were determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) from soils and plant sections of post-winter seedlings, anthesis-state, and mature plants to record within-plant levels of essential and toxic minerals during ripening and to estimate the (re)use of the soil-A herbage in husbandry and in HM-sensitive fermentations. Non-permissible HM loads (mg·kg −1 ·DW) of soil A in Cd, Cu, and Zn of 40.4, 261, and 2890, respectively, initiated the corresponding phytotoxic concentrations in roots and of Zn in shoots from the seedling state to maturity as well as of Cd in the foliage of seedlings. At anthesis, shoot concentrations in Ca, Cd, Fe, Mg, Mn, and Zn and in As, Cr, Pb, and U had fallen to a mean of 20% to increase to 46% during maturation. The respective shoot concentrations in C-grown plants diminished from anthesis (50%) to maturity (27%). They were drastically up/down-regulated at the rachis-grain interface to compose the genetically determined metallome of the grain during mineral relocations from adjacent sink tissues. Soil A caused yield losses of straw and grain down to 47.7% and 39.5%, respectively. Nevertheless, pronounced HM excluder properties made Cd concentrations of 1.6-3.08 in straw and 1.2 in grains the only factors that violated hygiene guidelines of forage (1). It is estimated that grains and the less-contaminated green herbage from soil A may serve as forage supplement. Applying soil A grains up to 3 and 12 in Cd and Cu, respectively, and the mature straw as bioenergy feedstock could impair the efficacy of ethanol fermentation by Saccharomyces cerevisiae.

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“…With further increase in the metal ion concentration above 20 mM, laccase activity decreased, but had detectable activity even at 25 mM. Copper and cadmium was also reported to increase the laccase activity in P. ostreatus (Baldrian et al 2000). Baldrian has discussed the sensitivity of laccases toward heavy metal ions as well (Baldrian 2006).…”

Section: Effect Of Metal Ionsmentioning

confidence: 99%

Assessing the tolerance of immobilized laccase from a salt-tolerant strain of Trichoderma viride Pers NFCCI-2745 to heavy metal ions, detergents and copper chelating agents

Divya

Prasanth

Sadasivan

2014

Int. J. Environ. Sci. Technol.

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The biotreatment of industrial effluents that contain heavy metals, detergents and metal chelating agents represents a great concern to industries as these compounds are either toxic to microbes or they act as noncompetitive inhibitors/denaturing agents for several enzymes. So, it is essential to know the effect of various agents usually present in the treatment plant on activity of the enzyme, for extending its applications in in situ treatment processes. This study describes the immobilization conditions of laccase and analysis of the sensitivity of immobilized laccase from Trichoderma viride Pers NFCCI-2745 to heavy metals, detergent and copper chelating agents. The concentrations and combinations of cations influenced the activity of immobilized enzyme and its yield. The immobilized Cu-Ba alginate enzyme showed comparatively higher activity compared to the Cu-alginate enzyme. Most of the metal ions tested enhanced laccase activity at lower concentration. Immobilized laccase retained more than 70 % of its activity even in the presence of 20 mM copper chelating agents like EDTA and sodium thioglycolic acid. Sodium azide, on the other hand, inhibited 80 % of the activity of all the beads even at a very low concentration of 0.5 mM. Cu-Ba and Cu-Ni alginate enzyme exhibited comparatively higher tolerance than Cualginate beads to EDTA and sodium thioglycolate. Tween 20 and cetyl trimethyl ammonium bromide significantly increased the activity of all the beads. These properties of the immobilized laccase may be aptly considered and suitably utilized in treating phenolic effluents that may contain heavy metals, detergents and certain copper chelating agents which may otherwise pose a threat to enzymatic activity.

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Influence of Cadmium and Mercury on Activities of Ligninolytic Enzymes and Degradation of Polycyclic Aromatic Hydrocarbons by Pleurotus ostreatus in Soil

Cited by 199 publications

References 63 publications

Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus – production of extracellular enzymes and characterization of the major cellulases

Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus – production of extracellular enzymes and characterization of the major cellulases

Gradual Accumulation of Heavy Metals in an Industrial Wheat Crop from Uranium Mine Soil and the Potential Use of the Herbage

Assessing the tolerance of immobilized laccase from a salt-tolerant strain of Trichoderma viride Pers NFCCI-2745 to heavy metal ions, detergents and copper chelating agents

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