INFLUENCE OF cAMP ON CELL DIFFERENTIATION AND MORPHOGENESIS IN POLYSPHONDYLIUM

Hohl, Hans R.; Honegger, Rosmarie; Traub, Franz; Markwalder, Martin

doi:10.1016/b978-0-444-41608-7.50020-4

Cited by 14 publications

(7 citation statements)

References 39 publications

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“…The number of vacuoles was gradually reduced as the cells moved into the anterior prestalk region. This was attributed to a fusion process which resulted in the formation of fewer but larger vacuoles, a phenomenon initially noted in P. paflidurn by Hohl et al [17]. However, the prespore region including the cells immediately adjacent to the prestalk cells, contained intact prespore vesicles (Fig.…”

Section: Resalts and Diclcassionmentioning

confidence: 66%

“…This possibility was verified by Bonner [4] who reported that vegetative amoebae responded to cyclic AMP by differentiating into mature stalk cells. Furthermore, P. pallidum prespores exposed to cyclic AMP redifferentiated into mature stalk cells [17]. It has been determined that a 5 min exposure of D. discoideum vegetative amoebae to cyclic AMP results in a 20% loss in their ability to synthesize glycoproteins [15].…”

Section: Resalts and Diclcassionmentioning

confidence: 99%

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Dynamics of Cell Redifferentiation in Dictyostelium mucoroides

Gregg

Davis

1982

Differentiation

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Section: Resalts and Diclcassionmentioning

confidence: 66%

Section: Resalts and Diclcassionmentioning

confidence: 99%

Dynamics of Cell Redifferentiation in Dictyostelium mucoroides

Gregg

Davis

1982

Differentiation

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“…cAMP has been shown to inhibit the development of pseudoplasmodia from fruiting body aggregates of P . pallidum at comparable concentrations (Hohl et al 1977). Hohl et al (1977) also observed that when P. pallidum WS320 vegetative amoebae were exposed to 1 X M cAMP on agar plates they formed cell aggregates which were surrounded by a sheath of material and contained differentiated stalk cells.…”

Section: Discussionmentioning

confidence: 89%

“…discoideum (Bonner 1970;Chia 1975) and P . pallidum (Francis 1975;Hohl et al 1977). The addition of cAMP to roller tube cultures of P .…”

Section: Discussionmentioning

confidence: 98%

Differentiation in roller tube cultures of wild-type and two cyclic AMP mutant strains of the cellular slime mould Polysphondylium pallidum

Paterno¹,

O’Day²

1982

Can. J. Microbiol.

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DAY. 1982. Differentiation in roller tube cultures of wild-type and two cyclic AMP mutant strains of the cellular slime mould Polysphondyliumpallidum. Can. J. Microbiol. 28: 1143-1 149. Submerged cultures of wild-type Polysphondylium pallidum (WS320) undergo a developmental sequence in which cells agglutinate and form tight aggregates within which extensive stalk and some spore differentiation occurs. Development of submerged cultures of P . pallidum bears many similarities to fruiting body cultures except that differentiation occurs in the absence of morphogenesis. Here we extend the results of an earlier study of submerged cultures of P. pallidum WS320 (Paterno and O'Day. 1981. Can. J. Microbiol. 27: 924-936) by showing that these cultures respond to several exogenous agents (cyclic AMP, lithium chloride, ammonium chloride, colchicine, and concanavalin A) in the same way as slime mould fruiting body cultures. However, two mutants abnormal in cyclic AMP production which complement to form fruiting bodies on agar plates could not form normal submerged culture aggregates when mixed together. Complementation tests with mutant and wild-type cells also failed. The inability of the mutants (PN507 and PN518) to complement in submerged cultures suggests that their fruiting complementarity may be based on a morphogenetic event. A low molecular weight fraction from wild-type cells could enhance development and stalk cell differentiation in WS320 and one mutant, PN507, but not in PN518. Together these data reveal that submerged cultures can be utilized to test the effects of extracellular factors on development and used as a source for the isolation of factors that regulate cellular differentiation. PATERNO, G. D., et D. H. O'DAY. 1982. Differentiation in roller tube cultures of wild-type and two cyclic AMP mutant strains of the cellular slime mould Polysphondyliumpallidum. Can. J. Microbiol. 28: 1143-1 149. Lorsque le type sauvage de Polysphondylium pallidum (WS320) est cultivk en immersion, il subit une succession de dkveloppements comportant l'agglutination des cellules et la formation d'aggrkgats compacts B l'intkrieur desquels surviennent le dkveloppement d'un p6doncule important et la diffkrenciation de spores. Le dkveloppement de P . pallidum en culture submergke ressemble en plus d'un point B celui des fructifications en culture, sauf pour la diffkrenciation qui se fait en I'absence de morphogentse. Ici, nous prolongeons les rksultats d'une ktude antkrieure sur la culture en immersion de P . pallidum WS320 (Paterno et O'Day. 1981. Can. J. Microbiol. 27: 924-936) et dkmontrons que, dans ce type de culture, l'organisme rkpond Bdivers agents exogknes (AMP cyclique, chlorure de lithium, chlorure d'ammonium, colchicine et concanavaline A) de la mSme faqon que les fructifications des moississures glissantes en culture. Toutefois, deux mutants mis ensemble en culture submergke n'ont pu dkvelopper des aggrkgats normaux en raison d'une anormalitk face B la production d'AMP cyclique, compliment nkcessaire B la formation d...

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“…The Polysphondylium violaceum chemoattractant, glorin, has recently been isolated and identified as a substituted dipeptide (glutamyl-ornithine) (21). Polysphondylium species do produce and excrete cyclic AMP during the early stages of development (2,7,10), but aggregating amoebae do not show enhanced chemotactic sensitivity toward cyclic AMP (11,12) as they do toward glorin (J. Bonner, personal communication). High concentrations of externally added cyclic AMP have been shown to delay slightly aggregation of P. violaceum (12) and to cause aberrant aggregation, stalk cell formation, and disruption of slugs in Polysphondylium pallidum (11).…”

mentioning

confidence: 99%

Extracellular cyclic AMP during development of the cellular slime mold Polysphondylium violaceum: comparison of accumulation in the wild type and an aggregation-defective mutant

Hanna¹,

Nowicki²,

Fatone³

1984

J Bacteriol

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Cyclic AMP was synthesized by Polysphondylium violaceum after starvation and during the preaggregation stage of development. Most of the newly synthesized cyclic AMP accumulated in the extracellular medium, with very little change in the intracellular cyclic AMP concentration. The addition of 10-3 to 10-6 M exogenous cyclic AMP to starved amoebae caused a 20 to 50% decrease in the number of aggregation centers formed compared with untreated controls. An aggregation-defective mutant of P. violaceum (strain aggA586) excreted or accumulated very little cyclic AMP. Strain aggA586 aggregated normally in the presence of a dialyzable, excreted product (D factor) produced by wild-type amoebae. When the mutant was incubated with D factor, cyclic AMP accumulated in the medium, and the amount accumulated depended on the amount of D factor added to the mutant amoebae.Cyclic AMP is involved in controlling development in the cellular slime molds (6,17). In Dictyostelium discoideum cyclic AMP is used as the chemoattractant for aggregate formation (13,14), affects stalk and spore cell differentiation (8), and is involved in gene regulation at the level of transcription (4,15,16,24). In Polysphondylium species the role of cyclic AMP is less well understood. Cyclic AMP is not the chemoattractant for aggregate formation (25). The Polysphondylium violaceum chemoattractant, glorin, has recently been isolated and identified as a substituted dipeptide (glutamyl-ornithine) (21). Polysphondylium species do produce and excrete cyclic AMP during the early stages of development (2, 7, 10), but aggregating amoebae do not show enhanced chemotactic sensitivity toward cyclic AMP (11, 12) as they do toward glorin (J. Bonner, personal communication). High concentrations of externally added cyclic AMP have been shown to delay slightly aggregation of P. violaceum (12) and to cause aberrant aggregation, stalk cell formation, and disruption of slugs in Polysphondylium pallidum (11). In this paper we show that the cyclic AMP synthesized by P. violaceum is rapidly excreted and that high extracellular concentrations of cyclic AMP lead to a decrease in the number of aggregation centers formed.In contrast to the effect of cyclic AMP on aggregation, an aggregation-stimulating factor, called D factor, is produced during the preaggregation period of development in the cellular slime molds (9). In P. violaceum and in D. discoideum production of D factor occurs just before synthesis and excretion of cyclic AMP (9, 10). Thus, in P. violaceum, both stimulatory and inhibitory chemicals are present during aggregation. A well-defined class of aggregation-defective mutants in P. violaceum (aggA) aggregate only in response to an external source of D factor (9). There is no morphological development by the aggA mutants in the absence of D factor, whereas in the presence of D factor aggregation and further development seem to be normal (9). As we show here, aggA mutants, in contrast to wild-type P. violaceum, synthesize and excrete little, if any, cyclic AMP. However, ...

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INFLUENCE OF cAMP ON CELL DIFFERENTIATION AND MORPHOGENESIS IN POLYSPHONDYLIUM

Cited by 14 publications

References 39 publications

Dynamics of Cell Redifferentiation in Dictyostelium mucoroides

Dynamics of Cell Redifferentiation in Dictyostelium mucoroides

Differentiation in roller tube cultures of wild-type and two cyclic AMP mutant strains of the cellular slime mould Polysphondylium pallidum

Extracellular cyclic AMP during development of the cellular slime mold Polysphondylium violaceum: comparison of accumulation in the wild type and an aggregation-defective mutant

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