Influence of Carbohydrate Accessibility on Cellulase Production by Lenzites trabea and Polyporus versicolor

Abstract

Cellulase and hemicellulase, both free and bound to cellulose, were produced in shake cultures of L. trabea and P. versicolor grown on ball-milled aspen (Populus tremuloides) or filter paper. Most of the enzyme was initially bound to cellulose plus mycelium and could not be readily dislodged, even by ultrasonic disintegration. Proportionally more enzyme could be obtained in the supernatant by reducing the cellulose concentration in the medium. No correlation was observed between the amount of growth and the amount of enzyme production. Rather, enzyme production was shown to be influenced by regulation of the growth rate through the use of cellulosic substrates of varying ease of accessibility and also when cultures were continuously supplied with glucose at a slow rate.

Submerged cultivation of edible white-rot fungi on tree bark

“…The resulting suspension was adjusted to pH 4.5 with 1.0 N NaOH and the flasks were autoclaved again. The final volume of 500 ml had a salts concentration equivalent to the original Hulme and Stranks (1974) medium.…”

“…prepared by growing the fungi on the salts medium of Hulme and Stranks (1974), and a substrate consisting of 1% malt extract and 0.5% ground filter paper (.Whatman CF 11).…”

“…After cooling, 330 ml of a 50% more concentrated Hulme and Stranks (1974) medium was added to each flask. The resulting suspension was adjusted to pH 4.5 with 1.0 N NaOH and the flasks were autoclaved again.…”