Influence of Carrier Materials and Storage Temperature on Survivability of Rhizobial Inoculant

Kaljeet, S.; Keyeo, F.; Amir, H. G.

doi:10.3923/ajps.2011.331.337

Cited by 35 publications

(18 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results indicated that the numbers of bacteria in third carrier remain stable during the storage period, and bacterial numbers were 9.910, 9.900 and 9.920 log 10 CFU/gm for incubation temperatures 5, 20 and 30 o C respectively. It is note that the optimum temperature to maintain the vitality of the bacterial inoculum was 30oC, These results are consistent with [40], where they noted that the temperature and humidity play an important role in pH adjustment and thus has a direct impact on the development and vitality of bacteria inside biofertilzers carriers.…”

Section: Survival Of Rhizobium Leguminosarumsupporting

confidence: 88%

Effect of Storage Temperature, Duration and Types of Biofertilizer Carriers on Survival and Numbers of Bacterial Strains Bacillus megaterium var. phosphaticum , Azotobacter chroococcum, Rhizobium leguminosarum and Transformant, Transconjugant B. megaterium var. phosphaticum

2016

Eminent Association of Pioneers (EAP) August 22-24, 2016 Kuala Lumpur (Malaysia)

View full text Add to dashboard Cite

this study was carried out in order to compare the different types of biofertilizers carriers, storage temperature and storage duration on the survival and numbers of bacterial strains in biofertilizers. Fifty soil samples and 25 nodules samples were collected from Erbil city, Kurdistan Region, Iraq. Bacillus megaterium var. phosphaticum, Azotobacter chroococcum, Rhizobium leguminosarum were isolated, purified and then identified by biochemical tests, isolated bacteria were capable of fixing atmospheric nitrogen and dissolving phosphate in the soil. Chromosomal DNA located nif genes responsible for nitrogen-fixing from Azotobacter chrococcum as a donor cell transferred to Bacillus megaterium var. phosphticuom as recipient cell by conjugation process. Plasmid DNA of Rhizobium leguminosarum with nod genes transferred to the conjugated cells by transformation process. The cells containing symbiotic nod genes and non-symbiotic nif genes were obtained and having the ability to dissolve phosphate and fixing atmospheric nitrogen. To ensure the successful of gene transfer the PCR technique was performed. Four types of bacteria were carried on the three carriers with different structures (carrier 1 = CaCo3 20% + Charcoal 20%+ Compost 20% + Clay 20% + Sand 19% + Gum1% , carrier 2 = Clay 50% + Compost 20% + CaCo3 20% + Charcoal 20% + Gum1% and carrier 3 = Sodium Alginate Sodium alginate solution (6% w/v)), and incubated for 6 months at three temperatures (5 o C, 20 o C and 30 o C) and the bacterial number was enumerated monthly. The results indicated that the number of bacterial cells increased gradually up to 60 days of incubation, then decreased and reached to 13.250 x10 10 Cell /gm of carrier at the end of incubation time from 8.333 x10 10 Cell/gm of starting time, while the number of bacterial Cell in sodium alginate carrier remain stable and survive up to the end of the incubation time.

show abstract

Section: Survival Of Rhizobium Leguminosarumsupporting

confidence: 88%

Effect of Storage Temperature, Duration and Types of Biofertilizer Carriers on Survival and Numbers of Bacterial Strains Bacillus megaterium var. phosphaticum , Azotobacter chroococcum, Rhizobium leguminosarum and Transformant, Transconjugant B. megaterium var. phosphaticum

2016

Eminent Association of Pioneers (EAP) August 22-24, 2016 Kuala Lumpur (Malaysia)

View full text Add to dashboard Cite

show abstract

“…Better survival was observed when bacteria were maintained at −18 C compared to 4 C or above. Kaljeet et al [20] also stored carrier materials (peat, rice husk and rice husk plus kaolin) which were inoculated with rhizobia at 4 C and 28 C for eight weeks and found that reduction of number of viable cell count is over a time less for peat compared to other tested materials. In experiments provided by Meade et al [5], viable cell numbers of bacteria stored in peat decreased steadily from 10 11 -10 12 CFU g −1 to 10 9 CFU g −1 or less during 26 weeks of storage at room temperature or at 4 °C.…”

Section: Resultsmentioning

confidence: 98%

Immobilization and Survival of Root Nodule Bacterium Rhizobium leguminosarum Biovar viciae / Gumiņbaktērijas Rhizobium leguminosarum biovar viciae imobilizācija un dzīvotspēja

Žvagiņa¹,

Petrina²,

Nikolajeva³

et al. 2015

Materials Science and Applied Chemistry

View full text Add to dashboard Cite

Rhizobium leguminosarum biovar viciae is a bacterium, which can establish nodules on roots of legumes. Rhizobial inoculants are used in agriculture as bio-fertilizers. Peat, clay powder and three kinds of expanded clay were tested for the immobilization of bacteria. The results showed that carrier material influences the success of immobilization and that storage temperature influences the survival. The best results were achieved with maintenance of bacteria in a suspension and immobilization on peat. We recommend storage of rhizobial products at a temperature of −18 C or 4 C.

show abstract

“…Earthworm activity is closely associated with microbial activity. Primarily moisture is playing a major role in microbial population maintenance as it has been reported by Prakash et al [27] and Kaljeet et al [28]. Tiunov and Scheu [29] have shown that earthworms deprive easily available carbon to microorganisms and availability of carbon increases effective mobilization of N and P by earthworms.…”

Section: Total Microbial Population and The Microbial Inoculantsmentioning

confidence: 96%

Microbial Enrichment of Vermicompost

Rajasekar

Daniel

2012

ISRN Soil Science

View full text Add to dashboard Cite

The present study has been conducted to explore the possibility of enrichment of vermicompost with microbial inoculants (i.e., biofertilizer organisms), Azospirillum brasilense and Rhizobium leguminosarum, optimization of inoculum level, and time of inoculation during vermicomposting. The survival rate of each microbial inoculant, total microbial population in vermicompost, and their correlation with the microbial inoculants during the storage period (180 days) were assessed. The change in population of A. brasilense and R. leguminosarum in vermicompost (at 30, 35, and 40 mL/175 g substrates) with reference to storage period showed highly significant negative correlation (P < 0.001). The total microbial population in A. brasilense and R. leguminosarum inoculated vermicompost was high during initial phases of storage and then total microbial population declined towards the end. The inoculum level of A. brasilense and R. leguminosarum at 35 mL per 175 g vermibed substrate is sufficient to maintain 1 × 10 7 viable cells up to 160 days after ther harvesting of vermicompost. The inoculum of these two biofertilizer organisms into vermibed on the 30th day showed increased survival rate and, hence, the optimized inoculation of 35 mL of inoculum per 175 g substrate on the 30th day of vermicomposting is helpful for the maintenance of sufficient viable population for more than five months in the enriched vermicompost.

show abstract

Influence of Carrier Materials and Storage Temperature on Survivability of Rhizobial Inoculant

Cited by 35 publications

References 12 publications

Effect of Storage Temperature, Duration and Types of Biofertilizer Carriers on Survival and Numbers of Bacterial Strains Bacillus megaterium var. phosphaticum , Azotobacter chroococcum, Rhizobium leguminosarum and Transformant, Transconjugant B. megaterium var. phosphaticum

Effect of Storage Temperature, Duration and Types of Biofertilizer Carriers on Survival and Numbers of Bacterial Strains Bacillus megaterium var. phosphaticum , Azotobacter chroococcum, Rhizobium leguminosarum and Transformant, Transconjugant B. megaterium var. phosphaticum

Immobilization and Survival of Root Nodule Bacterium Rhizobium leguminosarum Biovar viciae / Gumiņbaktērijas Rhizobium leguminosarum biovar viciae imobilizācija un dzīvotspēja

Microbial Enrichment of Vermicompost

Contact Info

Product

Resources

About