Influence of cellulase gene expression and cellulolytic activity on cellulose utilization in different Volvariella volvacea strains

Zhao, Yan; Liu, Shunjie; Li, Zhe; Zhang, Yaru; Li, Zhengpeng; Yu, Changxia; Chen, Mingjie

doi:10.1016/j.scienta.2019.108956

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…During Morchella’s growth and development, it secretes cellulase to hydrolyze cellulose into small glucose molecules, which are then absorbed and utilized by the Morchella mycelium and fruiting body. The activity of cellulase can reflect the physiological and biochemical activity of hyphae and fruiting body [ 28 ]. Edible fungi secrete laccase and other polyphenol oxidases to degrade lignin, producing small molecular nutrients and providing nutrition to promote mycelial growth [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

The effects of zinc sulfate on mycelial enzyme activity and metabolites of Pholiota adiposa

Ma,

Yang,

Xiao

et al. 2023

PLoS ONE

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The aim of this study was to investigate the effect of zinc sulphate on the activities of different enzymes and metabolites of Pholiota adiposa. In the experiment, we used the conventional enzyme activity assay to determine the changes of six indicators, including protein content, laccase activity, cellulase activity, amylase activity and polyphenol oxidase activity, under different concentrations of zinc sulphate treatment. The results showed that the activities of amylase, laccase, cellulase and peroxidase were Zn2+(200)>Zn2+(0)>Zn2+(400)>Zn2+(800).The activities of catalase and superoxide dismutase were Zn2+(200)>Zn2+(400)>Zn2+(800), and zinc sulfate could significantly affect the activity of polylipic squamase in a dose-dependent manner. Further correlation analysis showed that all six enzyme activities were significantly correlated with each other (P<001); the results of the statistical model test showed that the regression model constructed was statistically significant; overall the residuals met the conditions of normal distribution, and the corresponding points of different enzyme activities Q—Q’ were more evenly distributed around y = x, and all fell in the 90% acceptance interval, thus the series was considered to obey normal distribution; the results of the principal The results of the principal component analysis showed that principal component 1 was positively correlated with amylase, laccase and cellulase. Principal component 2 was positively correlated with superoxide dismutase and catalase, and negatively correlated with peroxidase. The analysis of Metabonomic data revealed that zinc sulfate had a significant impact on the expression of metabolites in the mycelium. Moreover, varying concentrations of zinc sulfate exerted significant effects on the levels of amino acids, organic acids, and gluconic acid. This conclusion was confirmed by other experimental data. The results of the study provide a scientific reference for better research, development and utilization of Pholiota adiposa.

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Section: Discussionmentioning

confidence: 99%

The effects of zinc sulfate on mycelial enzyme activity and metabolites of Pholiota adiposa

Ma,

Yang,

Xiao

et al. 2023

PLoS ONE

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“…When detecting acid and neutral proteases, the pH of buffer was adjusted to 4.0 and 7.0, respectively. Acid cellulase activity was also determined by measuring the amount of reducing sugars (Zhao et al ., 2020b). One unit of cellulase activity for BBQ was defined as the amount of enzyme that produced 1 mg of glucose per minute in the buffer (pH 5.0) at 50 °C.…”

Section: Methodsmentioning

confidence: 99%

Metaproteomic analysis of enzymatic composition in Baobaoqu fermentation starter for Wuliangye baijiu

Fan

Zhao

et al. 2021

Int J of Food Sci Tech

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Wuliangye is one of the most famous brands of baijiu in China. The typical flavours of Wuliangye baijiu are mainly formed by the multifarious enzymes in fermentation starter (Baobaoqu, BBQ). As the enzymes remained poorly understood, the detailed enzymatic composition of BBQ was analysed by metaproteomics for the first time. It is worth noting that the enzymes from plant, animals and micro-organisms were detected together and oxidoreductases were the dominant enzymes. Next, the analysis of function showed that the enzymes were mainly involved in carbon metabolism and the biosynthesis of amino acids. Additionally, the relationship between differential abundant enzymes and formation of typical flavour substances in premium and normal BBQ was established. Furthermore, the activities of classic enzymes in BBQ were examined to verify the reliability of metaproteomic results. This study will provide clues to further improve the manufacturing technology for Wuliangye baijiu.

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“…Volvariella volvacea uses the humus from the fermentation of crop straw (such as wheat stalk, corn stalk, etc.) as the main source of nutrition [9]. In the past three years, China's Volvariella volvacea production has increased year by year, and the output of China's Volvariella volvacea in 2021 was about 256,000 tons [10].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Influence of Culture Environment on the Yield of Volvariella volvacea Based on Microbiomics

Liu,

Wang,

Kang

et al. 2024

Horticulturae

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As one of the most nutritious and delicious mushroom varieties, Volvariella volvacea has always been popular among people around the world. Different from other artificially cultivated mushrooms, Volvariella volvacea is mostly planted on non-sterile substrates. As the cultivation time increases, the yield of mushroom houses continues to decrease. In our experiment, we selected two groups of samples from the old and new mushroom houses, environmental samples and substrate samples. The results showed that the diversity and abundance of microorganisms in the culture environment of the old mushroom room were consistent with that of the new mushroom room, but the proportion of actinomycetes in the former was significantly higher than that of the new mushroom room. The metabolic activity of microorganisms in the old mushroom room was enhanced compared with the new mushroom room. The microbial diversity in the growth substrate was investigated, and it was found that the structure and diversity of the microbial community in the substrate had changed. In conclusion, the interplay between mushrooms, the culture environment, and the host bacterial community may be the cause of the changes in the yield of Volvariella volvacea.

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Influence of cellulase gene expression and cellulolytic activity on cellulose utilization in different Volvariella volvacea strains

Cited by 9 publications

References 21 publications

The effects of zinc sulfate on mycelial enzyme activity and metabolites of Pholiota adiposa

The effects of zinc sulfate on mycelial enzyme activity and metabolites of Pholiota adiposa

Metaproteomic analysis of enzymatic composition in Baobaoqu fermentation starter for Wuliangye baijiu

Exploring the Influence of Culture Environment on the Yield of Volvariella volvacea Based on Microbiomics

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