Influence of chain length of N-acetyl-d-glucosamine and d-glucosamine residues on direct and complement-mediated chemotactic activities for canine polymorphonuclear cells

Usami, Yasuyuki; Minami, Saburo; Okamoto, Yoshiharu; Matsuhashi, Akira; Shigemasa, Yoshihiro

doi:10.1016/s0144-8617(96)00153-1

Cited by 56 publications

(30 citation statements)

References 14 publications

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“…The findings were supported by Azuma et al [4], who reported that chitosan promoted wound healing, Paul and Sharma [7] reported the haemostatic and re-ephitelization properties of chitosan at wound healing, Jayakumar et al [9] reported that chitosan formed barrier against infection and Usami et al [12] reported that wound healing was accelerated with the presence of chitosan by inducing the chemotaxis of nutrophils. The inflammatory cell infiltration with a remarkable accumulation of PMN was observed in the study, which was clearly reported by Usami et al [11][12][13] and Da Silva et al [14].…”

Section: The Clinical Responsesupporting

confidence: 85%

“…Additionally, it has been observed the chitosan's chemotactic activity promotes neutrophils without enforcing any antimicrobial activity directly to chitosan. This phenomenon may explain the chitosan as a biodegradable and bio-origin material which promotes the coagulation, early and late inflammation, proliferation and finally remodeling without any inflammatory response directly to itself [4,8,[12][13][14][15]24]. Finally, under the results of the case report, chitosan based wound materials were found to be good and effective in wound treatment and infection control in veterinary medicine, as reported earlier by Okamoto et al [15,16].…”

Section: Recoverymentioning

confidence: 52%

“…Cheung et al reported [10] bio-molecules of marine origin with a defense action against pathogenic viruses and fungi. Usami et al [11,12] reported that the bovine and canine polymorph nuclear cells in vitro activation induced by chitin and chitosan [13]. The ability of chitin fragments to regulate murine macrophage cytokine in vitro production was described by Da silva et al [14].…”

mentioning

confidence: 98%

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Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report

Tekelioğlu¹,

Çeli̇k²,

Yandim³

2017

JAST-A

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Abstract:The aim of this case report was to demonstrate the effectivity of chitosan as topical agent on the treatment of severely infected canine extremity wound. The dog was hit by a bus and presented severe friction wounds accompanying with bilateral hip and extremity fractures. Local and generalized infections were observed on the post operational period. The dog was unresponsive to the standard therapeutic protocols and health status was getting worsened. The regenerative sutures did not hold the tissues because of the infection and the tissue eruption. Chitosan samples were extracted from shrimp shell wastes. The milled form of chitosan was topically used to treat the open and heavily mix infected wound areas located at skin and rear extremities accompanying with fever and Canine coronavirus diarrhea. It was decided to use the chitosan as a bio barrier and anti-bio affect over the damaged and erupted tissues. Macroscopic findings indicated chitosan promoted the natural blood clotting and absorbed the inflammation fluid. Microscopic findings indicated the infiltration of polymorph nuclear leukocytes (PMN) accelerated with the topical use of chitosan in the early phase of wound healing. The wound healing was observed daily. This veterinary practice wound treatment result indicated that chitosan is effective to medicate topically the canine open and mix infected wounds with bacteria and viruses and promotes the granulomatosis in four weeks period.

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Section: The Clinical Responsesupporting

confidence: 85%

Section: Recoverymentioning

confidence: 52%

mentioning

confidence: 98%

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Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report

Tekelioğlu¹,

Çeli̇k²,

Yandim³

2017

JAST-A

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“…11,35 We also reported that chitin and chitosan enhance complement activity. 8 These results indicate that chitin and chitosan attract canine PMNs by at least four pathways in vivo: direct attraction, complement activation, arachidonic acid production, and cytokine production. Chitin and chitosan accelerate wound healing in humans and animals.…”

Section: Discussionmentioning

confidence: 79%

“…5,6 Wounds to which chitin and chitosan are applied show active and rapid accumulation of polymorphonuclear cells (PMNs), angiogenesis, and healing with little scarring. 7,8 Some investigators have reported on the mechanism by which chitin and chitosan act at a wounded site, which might involve activation of PMNs and macrophages, [9][10][11][12] a protective effect against microorganisms, 13 or promotion of granulation tissue formation with angiogenesis. 2,14 PMN migration is one of the important steps in wound healing, 15 and defects of PMN migration not only induce delayed wound healing but also severe infection.…”

Section: Introductionmentioning

confidence: 99%

Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2

Usami

Okamoto

Takayama

et al. 1998

J. Biomed. Mater. Res.

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The effects of chitin and chitosan on the release of arachidonic acid products were investigated in this study. Supernatants of canine polymorphonuclear cell (PMN) suspensions incubated with chitin and chitosan contained a leukotriene B4 (LTB4) concentration high enough to induce canine PMN migration in vitro. The supernatants also contained the same concentration of prostaglandin E2 (PGE2) as that normally found in the peripheral blood of dogs. Intraperitoneal administration of chitosan to dogs induced peritoneal exudative fluid (PEF), but chitin did not. The PEF contained numerous PMNs and macrophages. The supernatant of PEF contained both heat-stable and heat-labile chemotactic factors for canine PMNs. It also contained enough LTB4 to attract the canine PMNs in vitro.

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Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes

Lahiji

Sohrabi

Hungerford

et al. 2000

J. Biomed. Mater. Res.

406

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The search for biocompatible materials that can support the growth and phenotypic expression of osteoblasts and chondrocytes is a major challenge in the application of tissue engineering techniques for the repair of bone and cartilage defects. Chitosan, a copolymer of glucosamine and N-acetylglucosamine, may provide an answer to this search. Chitosan is the deacetylated product of chitin, a ubiquitous biopolymer found in the exoskeleton of insects and marine invertebrates. Little is known about the utility of chitosan in propagating human osteoblasts and chondrocytes. In this study, we test the hypothesis that chitosan promotes the survival and function of osteoblasts and chondrocytes. Chitosan (4%, w/v in 2% HAc) was coated onto plastic coverslips that had been fitted into 24-well plates. Human osteoblasts and articular chondrocytes were seeded on either uncoated or chitosan-coated coverslips at 1 x 10(5)/cells per well. Cultures were incubated at 37 degrees C, 5% CO(2) for a period of 7 days. Cell viability was assessed at that time using a fluorescent molecular probe. The phenotypic expression of osteoblasts and chondrocytes was analyzed by reverse transcriptase-polymerase chain reaction and immunocytochemistry. Osteoblasts and chondrocytes appeared spherical and refractile on chitosan-coated coverslips. In contrast, greater than 90% of cells on plastic coverslips were elongated and spindle shaped after 7 days of culture. Similar to cells propagated on uncoated control wells, greater than 90% of human osteoblasts and chondrocytes propagated on chitosan remained viable. Human osteoblasts propagated on chitosan films continued to express collagen type I whereas chondrocytes expressed collagen type II and aggrecan, as shown by reverse transcriptase-polymerase chain reaction analysis and immunostaining. The present in vitro work demonstrates the biocompatibility of chitosan as a substrate for the growth and continued function of human osteoblasts and chondrocytes. Chitosan may have potential use as a tissue engineering tool for the repair of osseous and chondral defects.

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Influence of chain length of N-acetyl-d-glucosamine and d-glucosamine residues on direct and complement-mediated chemotactic activities for canine polymorphonuclear cells

Cited by 56 publications

References 14 publications

Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report

Canine Extremity Wound Treatment with Chitosan Extracted from Shrimp Shells: A Case Report

Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2

Chitosan supports the expression of extracellular matrix proteins in human osteoblasts and chondrocytes

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