Influence of chicken growth hormone (cGH) SNP genotypes on morphometric and growth traits of three chicken breeds in Nigeria.

Abstract

This study was conducted to identify the diversity of chicken growth hormone (cGH) single nucleotide polymorphs’ (SNP) and their association on morphometric and growth traits of three Nigerian chicken breeds namely Funaab Alpha (FA), Shikabrown (SB) and Nigerian local chicken (NLC). Morphometric traits measured include shank length (cm), breast girth (cm) and breast width (cm) while growth traits measured were final body weight, av. weight gain, feed intake and feed conversion ratio all at laying stages respectively. Morphometric and growth traits were significantly influenced (P less than 0.05) between the different breeds. The SNP panel showed two base pair substitution mutation (GT and CA) on locus 5 chromosome for the FA and locus 6 chromosome for the SB and NLC respectively. The G – T alignment produced genotypes GG and GT, with TT not observed. Also C – A alignment produced genotypes CC and CA, with AA not observed. The NLC breed had the highest number of haplotypes of 6 while FA and SB had 4 haplotypes each implying that the local breed had more allelic variation than the other two breeds. Also FA had heterozygosity of 0.48 while SB and NLC had a value of 0.44, indicating higher genetic variability of FA breed than the rest with negative theta value (-0.0843) of F-statistics indicating high level of outbreeding. The conducted association of SNP genotypes showed no significant association effects (P>0.05) on the morphometric traits, except for SB breed where CA genotypes was significantly associated with CC for bodyweight, and in NLC breed where CC genotype was also significantly associated with CA genotypes for shank length.

Molecular Evolution of Prolactin Gene Single Nucleotide Polymorphisms in Nigerian Chicken Ecotypes and their Association with Light Ecotype Chickens’ Egg Traits

“…The result of this study compares favorably with the findings of (Osman et al 2017) which showed significant differences (P≤0.012) between the three chicken strains (Lohmann, Cobb 500, and Avian 48) with zero SNPs found and the Hubbard F15 strain, which contain three SNPs in PRL gene. Similarly, Okafor et al (2019) reported that the association of SNP genotypes showed no statistical association effects on all parameters evaluated, except for the CC genotype that was significantly associated with CA genotypes for shank length in the NIC.…”

“…However, to the best of our knowledge, this is the first work on SNP approach to the study of the Nigerian indigenous chicken prolactin gene and its association with Light Ecotype chicken's egg traits. Notwithstanding, association studies have been done on morphometric and growth traits in some breeds of the Nigerian chicken, including the indigenous birds (Okafor et al 2019;Bello et al 2020). In a recent study of Okafor et al (2019) the chicken growth hormone SNP was associated with morphometric and growth traits in the NIC in addition to Funaab Alpha and ShikaBrown.…”

“…Notwithstanding, association studies have been done on morphometric and growth traits in some breeds of the Nigerian chicken, including the indigenous birds (Okafor et al 2019;Bello et al 2020). In a recent study of Okafor et al (2019) the chicken growth hormone SNP was associated with morphometric and growth traits in the NIC in addition to Funaab Alpha and ShikaBrown. The SNP results revealed a two bp substitution mutation on locus 6 chromosome for the NIC.…”