Influence of chitosan and hydroxyapatite incorporation on properties of electrospun PVA/HA nanofibrous mats for bone tissue regeneration: Nanofibers optimization and in-vitro assessment

Salim, Samar A.; Loutfy, Samah A.; El‐Fakharany, Esmail M.; Taha, Tarek H.; Hussien, Yasmen; Kamoun, Elbadawy A.

doi:10.1016/j.jddst.2021.102417

Cited by 33 publications

(24 citation statements)

References 39 publications

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“…After specified time intervals, samples were removed, where extra water was wiped with soft tissue paper and weighted. The SR was calculated according to Salim et al 143 using the following equation where W i is the weight of dry samples (g) and W r is the swollen samples weight (g).…”

Section: Methodsmentioning

confidence: 99%

Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications

Saleh

El‐Gendi

El‐Fakharany

et al. 2022

Sci Rep

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The promising features of most bacterial celluloses (BC) promote the continuous mining for a cost-effective production approach toward wide and sustainable applications. Herein, cantaloupe peels (CP) were successfully implemented for sustainable BC production. Results indicated that the enzymatically hydrolyzed CP supported the maximum BC production of approximately 3.49 g/L when used as a sole fermentation media. The produced BC was fabricated with polyvinyl alcohol (PVA) and chitosan (Ch), and loaded with green synthesized copper oxide nanoparticles (CuO-NPs) to improve its biological activity. The novel composite showed an antimicrobial activity against several human pathogens such as Staphylococcus aureus, Streptococcus mutans, Salmonella typhimurium, Escherichia coli, and Pseudomonas fluorescens. Furthermore, the new composite revealed a significant in vitro anticancer activity against colon (Caco-2), hepatocellular (HepG-2), and breast (MDA) cancer cells, with low IC50 of 0.48, 0.27, and 0.33 mg/mL for the three cell lines, respectively. On the other hand, the new composite was remarkably safe for human skin fibroblast (HSF) with IC50 of 1.08 mg/mL. Interestingly, the composite membranes exhibited lethal effects against all stages of larval instar and pupal stage compared with the control. In this study, we first report the diverse potential applications of BC/PVA/Ch/CuO-NPs composites based on green synthesized CuO-NPs and sustainably produced BC membrane.

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Section: Methodsmentioning

confidence: 99%

Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications

Saleh

El‐Gendi

El‐Fakharany

et al. 2022

Sci Rep

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“…Numerous spinning conditions are required to achieve accepted morphological nanofibers in terms of smooth, beads less, nanofiber production. 33 PCL and PMMA were blended in different ratios then fabricated under different voltage, and adapted solution feed rate, as explained in Table 2 . Table 2 exhibits the spinning conditions optimization for preparation of nanofibrous scaffolds with different compositions.…”

Section: Resultsmentioning

confidence: 99%

“…Numerous spinning conditions are required to achieve accepted morphological nanofibers in terms of smooth, beads less, nanofiber production. 33 1. The collected sandwich nanofiber mats were dried using vacuum oven for 24 h to remove residual solvents and then were stored in dried, cold, conditions to avoid any possible contamination.…”

Section: Optimization Of Spinning Conditionsmentioning

confidence: 99%

Mercaptopurine-Loaded Sandwiched Tri-Layered Composed of Electrospun Polycaprolactone/Poly(Methyl Methacrylate) Nanofibrous Scaffolds as Anticancer Carrier with Antimicrobial and Antibiotic Features: Sandwich Configuration Nanofibers, Release Study and in vitro Bioevaluation Tests

Salim¹,

Kamoun²,

Evans³

et al. 2021

IJN

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Background: 6-Mercaptopurine (6-MP) is a potential anti-cancer agent which its therapeutic and limitation applicability due to its high toxicity. Objective: Herein, 6-MP was loaded into tri-layered sandwich nanofibrous scaffold (the top layer composed of poly methyl methacrylate/polycaprolactone (PMMA/PCL), the middle layer was PCL/PMMA/6-MP, and the bottom layer was PCL/PMMA to improve its bioactivity, adjusting the release-sustainability and reduce its toxicity. Methods: Electrospun tri-layered nanofibers composed of PCL/PMMA were utilized as nano-mats for controlling sustained drug release. Four groups of sandwich scaffold configurations were investigated with alteration of (PMMA: PCL) composition. Results:The sandwich scaffold composed of 2%PCL/4%PMMA/1%6-MP showed the best miscibility, good homogeneity and produced the smoothest nanofibers and low crystallinity. All fabricated 6-MP-loaded-PCL/PMMA scaffolds exhibited antimicrobial properties on the bacterial and fungal organisms, where the cytotoxicity evaluation proved the safety of scaffolds on normal cells, even at high concentration. Scaffolds provided a sustained-drug release profile that was strongly dependent on (PCL: PMMA). As (PCL: PMMA) decreased, the sustained 6-MP release from PCL/PMMA scaffolds increased. Results established that ~18% and 20% of 6-MP were released after 23h from (4%PCL/4%PMMA/1%6-MP) and (2%PCL/4%PMMA/1%6-MP), respectively, where this release was maintained for more than 20 days. The anti-cancer activity of all fabricated scaffolds was also investigated using different cancerous cell lines (e.g., Caco-2, MDA, and HepG-2) results showed that 6-MP-loaded-nanofibrous mats have an anti-cancer effect, with a high selective index for breast cancer. We observed that viability of a cancer cell was dropped to about 10%, using nanofibers containing 2% PCL/4%PMMA/1%6-MP. Conclusion: Overall, the PCL: PMMA ratio and sandwich configuration imparts a tight control on long-term release profile and initial burst of 6-MP for anticancer treatment purposes.

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“…All tested cells were measured at 570 nm using a microplate ELISA reader (BMG LabTech, Munich, Germany) and the relative cell viability (%) as compared with control wells containing cells without treatment was calculated using the following formula: (X) test/(Y) control × 100%. In addition, the sensitivity of crosslinked alginate/PVA/calcium phosphate (at pH 4 and 8) hybrid materials at concentrations of 0.5 and 1.0 mg/mL against the morphology of both HFB-4 and HSF cells was estimated by visualization under a phase contrast microscopy (Olympus, Hamburg, Germany) and compared to untreated control cells [ 38 , 39 ].…”

Section: Methodsmentioning

confidence: 99%

Mineralized Polyvinyl Alcohol/Sodium Alginate Hydrogels Incorporating Cellulose Nanofibrils for Bone and Wound Healing

et al. 2022

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Bio sustainable hydrogels including tunable morphological and/or chemical cues currently offer a valid strategy of designing innovative systems to enhance healing/regeneration processes of damaged tissue areas. In this work, TEMPO-oxidized cellulose nanofibrils (T-CNFs) were embedded in alginate (Alg) and polyvinyl alcohol (PVA) solution to form a stable mineralized hydrogel. A calcium chloride reaction was optimized to trigger a crosslinking reaction of polymer chains and mutually promote in situ mineralization of calcium phosphates. FTIR, XRD, SEM/EDAX, and TEM were assessed to investigate the morphological, chemical, and physical properties of different mineralized hybrid hydrogels, confirming differences in the deposited crystalline nanostructures, i.e., dicalcium phosphate dehydrate (DCPDH) and hydroxyapatite, respectively, as a function of applied pH conditions (i.e., pH 4 or 8). Moreover, in vitro tests, in the presence of HFB-4 and HSF skin cells, confirmed a low cytotoxicity of the mineralized hybrid hydrogels, and also highlighted a significant increase in cell viability via MTT tests, preferentially, for the low concentration, crosslinked Alg/PVA/calcium phosphate hybrid materials (<1 mg/mL) in the presence of hydroxyapatite. These preliminary results suggest a promising use of mineralized hybrid hydrogels based on Alg/PVA/T-CNFs for bone and wound healing applications.

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Influence of chitosan and hydroxyapatite incorporation on properties of electrospun PVA/HA nanofibrous mats for bone tissue regeneration: Nanofibers optimization and in-vitro assessment

Cited by 33 publications

References 39 publications

Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications

Exploitation of cantaloupe peels for bacterial cellulose production and functionalization with green synthesized Copper oxide nanoparticles for diverse biological applications

Mercaptopurine-Loaded Sandwiched Tri-Layered Composed of Electrospun Polycaprolactone/Poly(Methyl Methacrylate) Nanofibrous Scaffolds as Anticancer Carrier with Antimicrobial and Antibiotic Features: Sandwich Configuration Nanofibers, Release Study and in vitro Bioevaluation Tests

Mineralized Polyvinyl Alcohol/Sodium Alginate Hydrogels Incorporating Cellulose Nanofibrils for Bone and Wound Healing

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