Influence of Chitosan Concentration on Cell Viability and Proliferation in Vitro by Changing Film Topography

Abstract

These results establish that changes in chitosan concentration affects the polymer surface topography, which has a direct effect in the growing cell behavior. Additionally, higher concentration of chitosan gels are required to be used in neural tissue engineering.

Synthesis of cellulose nanocrystals from spinach waste for insulin delivery: comparison to chitosan nanoparticles

“…A research study confirmed the findings about chitosan, wherein C6 and PC12 cells proliferated and survived more readily when placed on 2% chitosan films. 31 Chitosan has been considered a potential material for nanobiomedical research since it has been demonstrated to be non-toxic, biodegradable, and biocompatible. However, tests with HeLa and MCF-7 cells demonstrated that chitosan nanoparticles caused cell death.…”

Biocompatibility analysis of high molecular weight chitosan obtained from Pleoticus muelleri shrimps. Evaluation in prokaryotic and eukaryotic cells

“…Previous results indicated that while CS alone favoured cell attachment and growth of human coronary artery endothelial cells, glycosaminoglycans-CS materials inhibited cell spreading and proliferation; meanwhile, dextran sulfate-CS stimulated cell proliferation [ 7 ]. On the other hand, murine bEnd5 brain endothelial cells showed no proliferation under commercial CS treatment [ 68 ]. As evidenced, there are many disparities about the cell growth behaviour depending on the type of CS used and the cell lines cultured.…”

“…Together, these findings denote how relevant could be the selection of in vitro experimental models. As indicated by Martín-López et al , cell growth depend not only on the molecular characteristics of CS but also on intrinsic factors of the employed cell type under study [ 68 ].…”

Peripheral Nerve Regeneration

“…Compositions of chitosan and gelatin have also been tested referring to the cell affinity and the potential to neurite outgrowth. Despite a decreased cell viability, chitosan blended with gelatine was able to show superior neurite outgrowth and in the first days of cell culture compared to PLL containing materials (Martín-López et al, 2012).…”