Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes

Lv, Xiaoyang; Liu, Yunen; Song, S. N.; Tong, Changci; Shi, Xiuyun; Zhao, Yan; Zhang, Jinsong; Hou, Mingxiao

doi:10.1016/j.carbpol.2018.10.067

Cited by 115 publications

(63 citation statements)

References 61 publications

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“…Different ways of associating alginate and chitosan have been de scribed in the literature: by mixing at room (Florczyk et al, 2011;Xu et al, 2019;Lv et al, 2019) or higher temperature (Fletcher et al, 2017), bilayer (Banerjee and Ganguly, 2019) or layer by layer (Silva et al, 2017) assemblies, crosslinking (Baysal et al, 2016;Naghizadeha et al, 2018, Zhang et al, 2018, 3D printing (Liu et al, 2018), … They lead to biocompatible buildings whose mechanical properties can be variable (Francis et al, 2013;Salehi et al, 2019;Lv et al, 2019;Xu et al, 2019), even up to correspond to the mechanical properties of hard tissues Florczyk et al, 2013). In this context our strategy was to let these biopolymers chemically unmodified, in order to maintain their excellent initial biocompatibility, and to work on the process to obtain a macroporous 3D architecture with improved rheo logical properties compared with reference polymers.…”

Section: Discussionmentioning

confidence: 99%

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Bushkalova

Farno

Tenailleau

et al. 2019

International Journal of Pharmaceutics

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In this study we evaluate macroporous scaffolds made of alginate-chitosan polyelectrolyte complexes (PEC) as tools to optimire the results of soft tissues oell therapy. Cell therapy using mesenchymal stem cells (MSC) bas become attractive for tissue repair and regeneration in a number of acute and chronic injuries. Unfortunately their low retention and/or survival after injection limit their beneficial effects. A biomaterial-assisted im plantation, providi ng oells a thr�mensional (3D) microenvironment is a promising strategy. To this purpose, we designed a family of PEC scaffolds, and studied if they could meet the requirement of such application. Xray tomography showed that ail PEC scaffolds present an interconnected macroporosity, and both rheology and tensile measurements reveal optimized mechanical properties (higher storage moduli and Young moduli) compared to alginate referenoe scaffolds. In vitro assays demonstrated their ability to allow MSC retention (higher than 90%) , lo ng-term viability and RiF2 secretion. Then, we used a skeletal muscle implantation mode! to assess the biological response to scaffolds graft, and showed that they support in vivo vascular formation within the implant-derived tissue. The combination of alginate/chitosan PEC scaffolds architecture and angio genic potential make them appear as interesti ng tools to optimize MSC therapy results in soft tissues.

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Section: Discussionmentioning

confidence: 99%

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Bushkalova

Farno

Tenailleau

et al. 2019

International Journal of Pharmaceutics

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“…The storage modulus allows for the determination of the hydrogel formation kinetics and also for distinctive analyses of stiff or soft hydrogels. A study by Lv et al [42] employed the ionic gelation approach by stirring varying stock carboxymethyl chitosan (CMCS) and alginate weight ratios (4:1, 2:1, 1.5:1,1:1, 0.67:1, 0.5:1, 0.25:1) and adding D-glucono-δ-lactone (GDL) at different time points [42]. CMCS/alginate/COS hydrogels were produced by the addition of varying chitosan oligosaccharide (COS) concentrations following the above ionic gelation approach [42].…”

Section: Fabrication Approaches Affecting Physical and Mechanical Promentioning

confidence: 99%

“…A study by Lv et al [42] employed the ionic gelation approach by stirring varying stock carboxymethyl chitosan (CMCS) and alginate weight ratios (4:1, 2:1, 1.5:1,1:1, 0.67:1, 0.5:1, 0.25:1) and adding D-glucono-δ-lactone (GDL) at different time points [42]. CMCS/alginate/COS hydrogels were produced by the addition of varying chitosan oligosaccharide (COS) concentrations following the above ionic gelation approach [42]. The addition of COS resulted in two-step hydrogel formation kinetics with a more porous morphology ( Figure 4III,IV) [42].…”

Section: Fabrication Approaches Affecting Physical and Mechanical Promentioning

confidence: 99%

Bioplatform Fabrication Approaches Affecting Chitosan-Based Interpolymer Complex Properties and Performance as Wound Dressings

et al. 2020

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Chitosan can form interpolymer complexes (IPCs) with anionic polymers to form biomedical platforms (BMPs) for wound dressing/healing applications. This has resulted in its application in various BMPs such as gauze, nano/microparticles, hydrogels, scaffolds, and films. Notably, wound healing has been highlighted as a noteworthy application due to the remarkable physical, chemical, and mechanical properties enabled though the interaction of these polyelectrolytes. The interaction of chitosan and anionic polymers can improve the properties and performance of BMPs. To this end, the approaches employed in fabricating wound dressings was evaluated for their effect on the property–performance factors contributing to BMP suitability in wound dressing. The use of chitosan in wound dressing applications has had much attention due to its compatible biological properties. Recent advancement includes the control of the degree of crosslinking and incorporation of bioactives in an attempt to enhance the physicochemical and physicomechanical properties of wound dressing BMPs. A critical issue with polyelectrolyte-based BMPs is that their effective translation to wound dressing platforms has yet to be realised due to the unmet challenges faced when mimicking the complex and dynamic wound environment. Novel BMPs stemming from the IPCs of chitosan are discussed in this review to offer new insight into the tailoring of physical, chemical, and mechanical properties via fabrication approaches to develop effective wound dressing candidates. These BMPs may pave the way to new therapeutic developments for improved patient outcomes.

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“…However, the cell viability tends to increase when exposed for 72 h, indicating that eventually cells keep growing due to an improvement in the biocompatibility, promoted as a positive characteristic in terms of prolonged drug delivery [21], which is the opposite for the case of adenocarcinoma cells. From the results, it is also important to take into account that increasing the Cs-Al NDs concentration may affect negatively the cell viability, as was observed for the treatment using 50 μg/mL which resulted in a slight inhabitation of both cell lines due to induced oxidative stress on cells or changes in the cell membrane, as well as inducing damage of the DNA [1,55,56]. These results are in quantitative agreement with the results reported in the literature, which obtained low growth inhibition using similar nanomaterials synthesized with chitosan and alginate biopolymers for cytotoxicity assessment [13,14,57].…”

Section: International Journal Of Polymer Sciencementioning

confidence: 99%

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

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We report on the synthesis of chitosan-alginate nanodisks (Cs-Al NDs) using a simple approach consisting of the ionotropic gelation method. Sodium tripolyphosphate (STPP) was used as crosslinking agent to promote the electrostatic interaction between amine groups the chitosan and hydroxyl and carboxyl groups of alginate. Scanning electron microscopy (SEM) images provided direct evidence of the morphology of the nanodisks where agglomeration was observed due to the electrostatic interaction between the functional groups. Furthermore, dynamic light scattering (DLS) showed that the hydrodynamic size of the Cs-Al NDs was 227 nm and 152 nm in pH 1.2 and pH 7.4, respectively, which is in agreement with the information observed in the SEM images. The chemical structure is presented mainly the amine and carboxyl groups due to the presence of chitosan and alginate in the nanodisks, respectively, which allow the electrostatic interaction through N-H linkages. According to the X-ray diffraction, we found that the Cs-Al NDs exhibited the typical structure of chitosan and alginate, which lead the formation of polyelectrolyte complexes. We also evaluated the encapsulation of amoxicillin in the nanodisk, obtaining a loading efficiency of 74.98%, as well as a maximum in vitro release amount of 63.2 and 52.3% at pH 1.2 and 7.4, respectively. Finally, the cytotoxicity effect of the Cs-Al nanodisks was performed in human prostatic epithelial PWR-1E and Caucasian prostate adenocarcinoma PC-3 cell lines, in which the cell viability was above 80% indicating low inhibition and determining the Cs-Al NDs as a promising technology for controlled delivery systems.

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Influence of chitosan oligosaccharide on the gelling and wound healing properties of injectable hydrogels based on carboxymethyl chitosan/alginate polyelectrolyte complexes

Cited by 115 publications

References 61 publications

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Alginate-chitosan PEC scaffolds: A useful tool for soft tissues cell therapy

Bioplatform Fabrication Approaches Affecting Chitosan-Based Interpolymer Complex Properties and Performance as Wound Dressings

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

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