The present study evaluated the influence of 2% chlorhexidine and 2.5% sodium hypochlorite on the resin sealer/dentin interface bond strength of AH Plus/gutta-percha and Epiphany/Resilon. Seventy-two extracted bovine incisors were randomly distributed into 6 groups according to irrigant and sealers: G S+AH =physiologic saline solution+AH Plus/gutta-percha; G S+Ep =physiologic saline solution+Epiphany/Resilon; G H+AH =2.5% sodium hypochlorite (NaOCl)+AH Plus/gutta-percha; G H+Ep =2.5% NaOCl+Epiphany/ Resilon; G C+AH =2% chlorhexidine (CHX)+AH Plus/gutta-percha; and G C+Ep =2% CHX +Epiphany/Resilon. After 7 days at 377C and 100% humidity, the roots were cut transversally on the long axis of the tooth in 0.8 mm (±0.09)-thick slices; these slices were then subjected to the push-out test. Data were analyzed using a 2-way ANOVA and Tukey tests at 5% significance. The AH Plus/gutta-percha groups showed significantly higher bond strength than the Epiphany/Resilon groups, regardless of the irrigant used (p<0.001). Sodium hypochlorite adversely affected bond strength in the AH Plus group, whereas chlorhexidine did not influence the push-out bond strength of either sealer (p<0.05). Two percent chlorhexidine did not adversely affect the bond strength of the sealers, whereas 2.5% sodium hypochlorite solution damaged AH Plus/gutta-percha bond strength.