Influence of cholesterol content on red cell membrane viscoelasticity and fluidity

Chabanel, A.; Flamm, Michael; Sung, K.L. Paul; Lee, M. M.; Schachter, David; Chien, Shu

doi:10.1016/s0006-3495(83)84288-x

Cited by 132 publications

(89 citation statements)

References 17 publications

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“…Both parameters are significantly increased. 111 terms of flexibility, this finding is in agreement with that of Chabanel et a1 (21) and was also found in rats experimentally exposed to lead (27). Concerning increased aggregation, no data are available.…”

Section: Discussionsupporting

confidence: 91%

“…Qualitatively, these viscoelastic curves imply erythrocytes showing an enhanced rigidity (18)(19)(20). Biochemical investigations have demonstrated that erythrocyte cholesterol is increased in lead-exposed workers (I I) and that the fluidity of the cholesterol-enriched erythrocytes is decreased (21). Moreover, in vitro experi~nents have shown that the membrane viscosity of erythrocytes is increased after incubation of ghost membranes for erythrocytes with lead for 1 h (22).…”

Section: Discussionmentioning

confidence: 99%

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Hemorheology in occupational lead exposure

Osterode

1996

Scand J Work Environ Health

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Objectives Tlle purpose of this investigation was to study hemorheological parameters in occupationally lead exposed men. Material and methods For 15 clinically healthy lead-exposed male subjects [age 34.6 (SD 8) years] the viscous (E') and elastic (E") components of whole blood viscosity corrected for 45% hematocrit were measured for shear rates between 1 . s-I -100 . s-' at 37OC. Moreover, lead concentrations in blood and urine and the deltaaminolevuliilic acid dehydrase level were determined. Fifteen unexposed age-matched men were used as referents.Results The meail lead concentration in the blood and urine of the lead-exposed men was 48.7 (SD 16.2) pg . dl-' and 38.8 (SD 17.1) pg . g creatinine-', respectively. While E" was significantly increased only at low shear rates, E' was elevated throughout the investigated shear range in comparison with the values of the unexposed referents. These results are characteristic of erythrocytes with enhanced rigidity. The aggregation and filtration index of erythrocytes were significantly elevated for the lead-exposed workers. A positive correlation existed between lead in blood and E' or E", whereas 6-aminolevulinic acid dehydrase and E' or E" were inversely correlated. No differences in plasma viscosity or in lipid status could be detected. C O~C~U S~O~Increased blood lead concentrations can be considered an additional risk factor for vascular diseases predisposing towards microvascular occlusion.Key terms blood viscosity, erythrocyte, lead, 6-aminolevulinic acid dehydrase.Approximately 95% of the circulating fraction of lead in the human body is bound to erythrocytes (I). Several

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Hemorheology in occupational lead exposure

Osterode

1996

Scand J Work Environ Health

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“…Membrane lipid order is influenced by various factors, including changes in lipid composition, the concentration of membrane cholesterol, the density of lipid packing, relative water content, ion concentration, pH, and temperature, and shear stress may alter membrane lipid order via any of these factors or combinations of them. Studies on living cells and membrane models have shown that the cholesterol content of the membrane has a major impact on the physical properties of plasma membranes, including on their membrane fluidity (Chabanel et al, 1983;Coderch et al, 2000). In the present study, treatment of ECs with MbCD, which depletes membrane cholesterol, was found to decrease the membrane lipid order, and the addition of cholesterol prevented the effects of MbCD.…”

Section: Discussionsupporting

confidence: 51%

Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases

Yamamoto

Ando

2013

Journal of Cell Science

108

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SummaryEndothelial cells (ECs) sense shear stress and transduce blood flow information into functional responses that play important roles in vascular homeostasis and pathophysiology. A unique feature of shear-stress-sensing is the involvement of many different types of membrane-bound molecules, including receptors, ion channels and adhesion proteins, but the mechanisms remain unknown. Because cell membrane properties affect the activities of membrane-bound proteins, shear stress might activate various membrane-bound molecules by altering the physical properties of EC membranes. To determine how shear stress influences the cell membrane, cultured human pulmonary artery ECs were exposed to shear stress and examined for changes in membrane lipid order and fluidity by Laurdan two-photon imaging and FRAP measurements. Upon shear stress stimulation, the lipid order of EC membranes rapidly decreased in an intensity-dependent manner, and caveolar membrane domains changed from the liquid-ordered state to the liquid-disordered state. Notably, a similar decrease in lipid order occurred when the artificial membranes of giant unilamellar vesicles were exposed to shear stress, suggesting that this is a physical phenomenon. Membrane fluidity increased over the entire EC membranes in response to shear stress. Addition of cholesterol to ECs abolished the effects of shear stress on membrane lipid order and fluidity and markedly suppressed ATP release, which is a well-known EC response to shear stress and is involved in shear-stress Ca 2+ signaling. These findings indicate that EC membranes directly respond to shear stress by rapidly decreasing their lipid phase order and increasing their fluidity; these changes could be linked to shear-stress-sensing and response mechanisms.

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“…The effect of lipid composition modulation on red blood cells has been extensively studied. Indeed, the rheology of erythrocytes is altered in patients with hypercholesterolemia due to an in- creased cholesterol:PL ratio and impaired membrane fluidity (23,24). Statin treatment lowers the cholesterol amount in the membranes and normalizes erythrocyte membrane fluidity (10,25).…”

Section: Discussionmentioning

confidence: 99%

Phosphatidylethanolamine Is a Key Regulator of Membrane Fluidity in Eukaryotic Cells

Dawaliby

Trubbia

Delporte

et al. 2016

Journal of Biological Chemistry

306

202

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Adequate membrane fluidity is required for a variety of key cellular processes and in particular for proper function of membrane proteins. In most eukaryotic cells, membrane fluidity is known to be regulated by fatty acid desaturation and cholesterol, although some cells, such as insect cells, are almost devoid of sterol synthesis. We show here that insect and mammalian cells present similar microviscosity at their respective physiological temperature. To investigate how both sterols and phospholipids control fluidity homeostasis, we quantified the lipidic composition of insect SF9 and mammalian HEK 293T cells under normal or sterol-modified condition. As expected, insect cells show minimal sterols compared with mammalian cells. A major difference is also observed in phospholipid content as the ratio of phosphatidylethanolamine (PE) to phosphatidylcholine (PC) is inverted (4 times higher in SF9 cells). In vitro studies in liposomes confirm that both cholesterol and PE can increase rigidity of the bilayer, suggesting that both can be used by cells to maintain membrane fluidity. We then show that exogenously increasing the cholesterol amount in SF9 membranes leads to a significant decrease in PE:PC ratio whereas decreasing cholesterol in HEK 293T cells using statin treatment leads to an increase in the PE:PC ratio. In all cases, the membrane fluidity is maintained, indicating that both cell types combine regulation by sterols and phospholipids to control proper membrane fluidity.

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Influence of cholesterol content on red cell membrane viscoelasticity and fluidity

Cited by 132 publications

References 17 publications

Hemorheology in occupational lead exposure

Hemorheology in occupational lead exposure

Endothelial cell and model membranes respond to shear stress by rapidly decreasing the order of their lipid phases

Phosphatidylethanolamine Is a Key Regulator of Membrane Fluidity in Eukaryotic Cells

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