Influence of Chronic Obstructive Pulmonary Disease on Volatile Organic Compounds in Patients with Non-Small Cell Lung Cancer

Muñoz-Lucas, M. Ángeles; Jareño-Esteban, Javier; Gutiérrez-Ortega, Carlos; López-Guijarro, Pablo; Collado-Yurrita, Luis; Quintana-Díaz, Manuel; Callol-Sánchez, Luis Miguel

doi:10.1016/j.arbr.2020.10.004

Cited by 10 publications

(8 citation statements)

References 25 publications

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“…Despite being of utmost importance to obtain precise results, the addition of an internal standard (IS) to the samples was performed in few studies ( Basanta et al, 2012 ; Corradi et al, 2015 ; Fowler et al, 2015 ; Berna et al, 2021 ; Ibrahim et al, 2021 ; Van Oort et al, 2022 ; Ahmed et al, 2023 ; Cen et al, 2023 ; Myers et al, 2023 ) ( Tables 1 , 3 ; Supplementary Table S1 ). Likewise, hexamethylcyclotrisiloxane, a desorption tube bleeding compound, was used as an internal reference compound ( Callol-Sanchez et al, 2017 ; Jareño-Esteban et al, 2017 ; Muñoz-Lucas et al, 2020 ). The ISs used include not isotopically labeled compounds such as 1,4-difluorobenzene, 2-methylpentanal, 4-bromofluorobenzene, bromochloromethane, and stable isotopically labeled ones, such as acetone-d8, 1,4-dichlorobenzene-d4, chlorobenzene-d5, bromobenzene-d5, hexane-d14, n-heptane-d16, n-octane-d18, phenanthrene-d10, styrene-d8, toluene-d8, and xylene-d10.…”

Section: Methodologiesmentioning

confidence: 99%

GC-MS-based metabolomics of volatile organic compounds in exhaled breath: applications in health and disease. A review

Bajo-Fernández,

Souza-Silva,

Barbas

et al. 2024

Front. Mol. Biosci.

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Exhaled breath analysis, with particular emphasis on volatile organic compounds, represents a growing area of clinical research due to its obvious advantages over other diagnostic tests. Numerous pathologies have been extensively investigated for the identification of specific biomarkers in exhalates through metabolomics. However, the transference of breath tests to clinics remains limited, mainly due to deficiency in methodological standardization. Critical steps include the selection of breath sample types, collection devices, and enrichment techniques. GC-MS is the reference analytical technique for the analysis of volatile organic compounds in exhalates, especially during the biomarker discovery phase in metabolomics. This review comprehensively examines and compares metabolomic studies focusing on cancer, lung diseases, and infectious diseases. In addition to delving into the experimental designs reported, it also provides a critical discussion of the methodological aspects, ranging from the experimental design and sample collection to the identification of potential pathology-specific biomarkers.

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Section: Methodologiesmentioning

confidence: 99%

GC-MS-based metabolomics of volatile organic compounds in exhaled breath: applications in health and disease. A review

Bajo-Fernández,

Souza-Silva,

Barbas

et al. 2024

Front. Mol. Biosci.

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“…Despite over 8 million deaths per year that can be directly attributed to it, there are still about 1.3 billion smokers worldwide. 1 Smoking is the main cause of mortality due to cardiovascular diseases like atherosclerosis and its consequences, 2 respiratory disorders such as chronic obstructive bronchopathy (COPD), 3 , 4 chronic bronchitis and pulmonary emphysema, 5 bone disorders such as osteoporosis 6 , 7 as well as cancer, particularly lung, bladder, liver, breast and oral malignancies. 8 , 9 Consequently, governments worldwide have promoted anti‐smoking campaigns and laws.…”

Section: Introductionmentioning

confidence: 99%

Heat‐not‐burn tobacco (IQOS), oral fibroblasts and keratinocytes: cytotoxicity, morphological analysis, apoptosis and cellular cycle. An in vitro study

Pagano

Negri

Coniglio

et al. 2021

J of Periodontal Research

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Objectives The aim of this work is to investigate the biological effects of IQOS smoking on human gingival fibroblasts and human keratinocytes analysing cell viability, morphology, migration, apoptosis and cell cycle. Background Electronic cigarettes and tobacco heating systems have been marketed to reduce smoking damages caused by combustion. Methods Human gingival fibroblasts and human keratinocytes viability was determined by a colorimetric assay measuring mitochondrial dehydrogenase activity (MTT assay); after an in vitro exposure of 24 h, cell morphology was analysed with scanning electron microscope and cell migration was tested by Scratch assay, a method to mimic the migration of the cells during wound healing in vivo. Apoptosis and cell cycle were analysed with flow cytometry, and the expression of related genes (p53, Bcl2, p16 and p21) was indagated using real‐time polymerase chain reaction. Results IQOS extracts increased both cell viability (23%‐41% with fibroblasts and 30%‐79% with keratinocytes) and migration. No morphological alterations were observed. IQOS extracts did not induced an increase in cell death, but rose the number of S‐ and G2/M‐phase cells. IQOS extracts also significantly increased p53 expression by fibroblasts (undiluted and 6.25% dilution, 2‐ and 3.6‐fold higher, respectively) and reduced both Bcl2 (about two‐ and fivefold, respectively) and p21 expressions (about twofold with both extracts), while on keratinocytes both undiluted and 6.25% dilution extracts increased Bcl2 expression (about four‐ and threefold higher, respectively) and reduced p53 expression (about two‐ and fivefold, respectively). Conclusion IQOS smoke seemed to induce proliferation as highlighted by a viability assay, and migration and cell cycle analysis. The increased cell proliferation induced by IQOS devices must be carefully investigated for its possible clinical effects on oral cell populations.

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“…LC is subdivided into different categories with different clinical characteristics as well. Small cell LC (SCLC), with 20–25% percentage of occurrence [ 208 ], is characterized by increased metabolic and proliferation rates compared to other cancer cells [ 209 ], while non-small cell LC (NSCLC) accounts for 70–75% of LC cases and is subdivided into the smoking-related [ 37 , 210 ] squamous cell carcinoma (SCC) [ 208 ] and the non-squamous cell carcinomas [ 37 ] including adenocarcinomas (ADC) (minor smoking correlation) and large-cell carcinoma (LCC) [ 208 ]. Consequently, the accurate discrimination of different lung diseases and subtypes of a lung disease, especially using breath analysis of exhaled VOCs, is of particular importance.…”

Section: Differential Diagnosis and Disease Phenotyping And Staging I...mentioning

confidence: 99%

Breath Analysis: A Promising Tool for Disease Diagnosis—The Role of Sensors

Kaloumenou

Skotadis

Lаgopati

et al. 2022

Sensors

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Early-stage disease diagnosis is of particular importance for effective patient identification as well as their treatment. Lack of patient compliance for the existing diagnostic methods, however, limits prompt diagnosis, rendering the development of non-invasive diagnostic tools mandatory. One of the most promising non-invasive diagnostic methods that has also attracted great research interest during the last years is breath analysis; the method detects gas-analytes such as exhaled volatile organic compounds (VOCs) and inorganic gases that are considered to be important biomarkers for various disease-types. The diagnostic ability of gas-pattern detection using analytical techniques and especially sensors has been widely discussed in the literature; however, the incorporation of novel nanomaterials in sensor-development has also proved to enhance sensor performance, for both selective and cross-reactive applications. The aim of the first part of this review is to provide an up-to-date overview of the main categories of sensors studied for disease diagnosis applications via the detection of exhaled gas-analytes and to highlight the role of nanomaterials. The second and most novel part of this review concentrates on the remarkable applicability of breath analysis in differential diagnosis, phenotyping, and the staging of several disease-types, which are currently amongst the most pressing challenges in the field.

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Influence of Chronic Obstructive Pulmonary Disease on Volatile Organic Compounds in Patients with Non-Small Cell Lung Cancer

Cited by 10 publications

References 25 publications

GC-MS-based metabolomics of volatile organic compounds in exhaled breath: applications in health and disease. A review

GC-MS-based metabolomics of volatile organic compounds in exhaled breath: applications in health and disease. A review

Heat‐not‐burn tobacco (IQOS), oral fibroblasts and keratinocytes: cytotoxicity, morphological analysis, apoptosis and cellular cycle. An in vitro study

Breath Analysis: A Promising Tool for Disease Diagnosis—The Role of Sensors

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