The aim of this work was to analyze spatial phenology and grape quality variability related to the climatic characteristics within the Ribera del Duero Designation of Origin (DO). Twenty plots planted with cv. Tempranillo and distributed within the DO were analyzed for phenology from 2004 to 2013. Grape quality parameters at ripening (berry weight, sugar content, acidity and pH, and anthocyanins) were analyzed in 26 plots for the period 2003-2013. The relationships between phenology and grape parameters with different climatic variables were confirmed with a multivariate analysis. On average, bud break was April 27(th), bloom June 17(th), and veraison August 12th. However, phenology during the time period showed high variability, with differences between years of up to 21 days for a phenology stage. The earliest dates were observed in dry years (2005, 2006, and to a lesser degree in 2009) while the later phenology dates occurred in the wettest year of the period (2008). High correlations were found between veraison date and temperature variables as well as with precipitation-evapotranspiration recorded during the bloom-veraison period. These effects tended to be higher in the central part of the DO. Grape quality parameters also showed high variability among the dry and the wet years, and the influence of extreme temperatures on color development as well as the effect of available water on acidity were observed.