Influence of Clonal Variation on the Efficiency of Arundo donax Propagation Methods

Danelli, Tommaso; Cantaluppi, Enrico; Tosca, A.; Cassani, E.; Landoni, M.; Bosio, Stefano; Adani, Fabrizio; Pilu, Roberto

doi:10.1007/s00344-019-09947-4

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Once efficient protocols to overcome these technical limits become available, the recent advances in A. donax L. molecular markers [13,15,55,57,58] and transcriptomics [63,101,103,105,107] could be used to improve several important traits of this energy crop, for example herbicide Literature data suggest that clonal selection is the best effective method up to now for selection towards yield [57,65], in vitro propagation efficiency [67], salt tolerance [61,63,70] and phytoremediation [76][77][78]. Physical/chemical mutagenesis [93] and somaclonal variation [79,83] could represent opportunities to generate new genetic variability, although, since A. donax L. is a polyploid species, it may be very difficult to genetically modify it and overcome the redundancy of genetic information.…”

Section: Discussionmentioning

confidence: 99%

“…Significant differences were reported among clones, considering the percentage of node buds sprouting in hydroponic propagation, which ranged from 19.6% to 71.7%, and the number of shoots produced in a 45-day cycle of tissue culture, which ranged from less than 10 to over 40. The clone Ad20 gave the best performance during hydroponic cultivation and had a high predisposition towards micropropagation [67].…”

Section: Clonal Selectionmentioning

confidence: 99%

“…Different methods for A. donax tissue culture, essential in the frame of its genetic improvement, have been investigated by different authors. Large scale shoot culture micropropagation of A. donax is rapidly achievable starting from stem nodes, preferably sampled in autumn, followed by subcultures on a modified Murashige and Skoog (MS) medium supplemented with BAP (benzylaminopurine) concentrations of 2-3 mg/L [84], 0.3 mg/L [85], 0.5-5 mg/L [86], 2.5-5 mg/L [87] or 1.25-5mg/L [67], with roots developing on the same shoot propagation medium. Successful acclimatization (>95% survival) of plantlets can be carried out, even in late winter, in a cold greenhouse or under simpler facilities such as shade nets [84].…”

Section: Somaclonal Variation and Mutagenesismentioning

confidence: 99%

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Genetic Improvement of Arundo donax L.: Opportunities and Challenges

Danelli

Laura²,

Savona³

et al. 2020

Plants

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Arundo donax L., the giant reed—being a long-duration, low-cost, non-food energy crop able to grow in marginal lands—has emerged as a potential alternative to produce biomass for both energy production, with low carbon emissions, and industrial bioproducts. In recent years, pioneering efforts have been made to genetically improve this very promising energy crop. This review analyses the recent advances and challenges encountered in using clonal selection, mutagenesis/somaclonal variation and transgenesis/genome editing. Attempts to improve crop yield, in vitro propagation efficiency, salt and heavy metal tolerance by clonal selection were carried out, although limited by the species’ low genetic diversity and availability of mutants. Mutagenesis and somaclonal variation have also been attempted on this species; however, since Arundo donax is polyploid, it is very difficult to induce and select promising mutations. In more recent years, genomics and transcriptomics data are becoming available in Arundo, closing the gap to make possible the genetic manipulation of this energy crop in the near future. The challenge will regard the functional characterization of the genes/sequences generated by genomic sequencing and transcriptomic analysis in a complex polyploid genome.

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Section: Discussionmentioning

confidence: 99%

Section: Clonal Selectionmentioning

confidence: 99%

Section: Somaclonal Variation and Mutagenesismentioning

confidence: 99%

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Genetic Improvement of Arundo donax L.: Opportunities and Challenges

Danelli

Laura²,

Savona³

et al. 2020

Plants

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“…Alamo; and as control, the mixed meadows (MM) species already present in the area. The A. donax L. clone Ad20 represents a promising genotype for large scale cultivation, due to its production potential and efficient propagation [31][32][33]. The control MM was constituted by the predominant species Medicago sativa and Chenopodium album.…”

Section: Plant Materialsmentioning

confidence: 99%

Arundo donax L. Biomass Production in a Polluted Area: Effects of Two Harvest Timings on Heavy Metals Uptake

et al. 2021

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Within the framework of energy biomass production, Arundo donax L. is very promising for its capability to grow on marginal lands with high yields. This potential can be realized in unused polluted areas where the energy production can be coupled with phytoremediation, and harvested biomass represents a resource and a means to remove contaminants from the soil. Two main processes are considered to evaluate A. donax L. biomass as an energy crop, determined by the timing of harvest: anaerobic digestion with fresh biomass before winter and combustion (e.g., pyrolysis and gasification) of dry canes in late winter. The aim of this work was to evaluate the use of A. donax L. in an area polluted by heavy metals for phytoextraction and energy production at two different harvest times (October and February). For that purpose, we established in polluted area in northern Italy (Caffaro area, Brescia) an experimental field of A. donax, and included switchgrass (Panicum virgatum L.) and mixed meadow species as controls. The results obtained by ICP-MS analysis performed on harvested biomasses highlighted a differential uptake of heavy metals depending on harvest time. In particular, considering the yield in the third year, A. donax was able to remove from the soil 3.87 kg ha−1 of Zn, 2.09 kg ha−1 of Cu and 0.007 kg ha−1 of Cd when harvested in October. Production of A. donax L. for anaerobic digestion or combustion in polluted areas represents a potential solution for both energy production and phytoextraction of heavy metals, in particular Cu, Zn and Cd.

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