Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma

Tomeková, Juliána; Kyzek, Stanislav; Medvecká, Veronika; Gálová, Eliška; Zahoranová, Anna

doi:10.1007/s11090-020-10109-8

Cited by 48 publications

(50 citation statements)

References 44 publications

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“…There were significant differences for the treatment times of 60, 90 and 120 s, compared to NC ( Figure 8 ). This is also in accordance with [ 6 ], where seedlings pre-treated with oxygen CAPP (DCSBD; 400 W, 14 kHz) had higher level of DNA damage compared to ambient air CAPP treatment. CAPP generated in nitrogen caused the highest DNA damage from all working gases studied in this work.…”

Section: Resultssupporting

confidence: 91%

“…This is stated, for example, in [ 11 ], where the use of CAPP generated in a nitrogen atmosphere for 180 and 300 s led to a significant increase in water intake, high lactate and alcohol dehydrogenase activities and thus to a complete inhibition of pea seed germination. The study presented in [ 6 ] confirmed that the generation of DCSBD plasma, used in our work, in a nitrogen and ambient air atmosphere produces ultraviolet (UV) radiation which is harmful in high doses, as confirmed by the work [ 29 ]. Nitrogen plasma produces a greater deal of intensive UV radiation than ambient air plasma what could be the reason of low germination of seeds treated by nitrogen plasma at 90 s and 120 s. In other treated variants, an increase in percentage of germination (compared to the untreated control) was recorded, statistically significant increase was in the variants N30, O60, O90.…”

Section: Resultssupporting

confidence: 81%

“…The balance between production and detoxification of ROS may be disturbed by exposure to stronger environmental factors [ 36 , 37 ]. Tomeková et al [ 6 ], using FTIR spectroscopy, pointed to production of ozone (O 3 ) during CAPP generation in oxygen atmosphere and nitrogen oxides during CAPP generation in ambient air. Like UV radiation, long-term exposure to O 3 or reactive nitrogen species leads to oxidative stress and to partial or permanent disruption of homeostasis [ 38 , 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…In the nitrogen atmosphere, small amounts of N 2 O were observed because of oxygen impurities. In the oxygen atmosphere, only the presence of ozone was recorded [ 6 ]. Treatment of seed surfaces by non-thermal plasma generated in different gases effectively reduces the amount of pathogenic microorganism, which colonize corn grains [ 7 ], nuts [ 8 , 9 ], barley and wheat grains [ 1 ], and pine seeds [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Švubová

Slováková

Holubová

et al. 2021

Plants

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The present study aims to define the effects of Cold Atmospheric Pressure Plasma (CAPP) exposure on seed germination of an agriculturally important crop, soybean. Seed treatment with lower doses of CAPP generated in ambient air and oxygen significantly increased the activity of succinate dehydrogenase (Krebs cycle enzyme), proving the switching of the germinating seed metabolism from anoxygenic to oxygenic. In these treatments, a positive effect on seed germination was documented (the percentage of germination increased by almost 20% compared to the untreated control), while the seed and seedling vigour was also positively affected. On the other hand, higher exposure times of CAPP generated in a nitrogen atmosphere significantly inhibited succinate dehydrogenase activity, but stimulated lactate and alcohol dehydrogenase activities, suggesting anoxygenic metabolism. It was also found that plasma exposure caused a slight increment in the level of primary DNA damage in ambient air- and oxygen-CAPP treatments, and more significant DNA damage was found in nitrogen-CAPP treatments. Although a higher level of DNA damage was also detected in the negative control (untreated seeds), this might be associated with the age of seeds followed by their lower germination capacity (with the germination percentage reaching only about 60%).

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Švubová

Slováková

Holubová

et al. 2021

Plants

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“…A greater formation of single‐strand breaks after plasma treatment was confirmed also on the plasmid DNA. [ 67–70 ] Kyzek et al [ 71 ] and Tomeková et al [ 72 ] also observed increased DNA damage in pea seedlings after DCSBD plasma treatment of their seeds. In all these mentioned studies, the effect of direct plasma treatment on DNA was investigated.…”

Section: Resultsmentioning

confidence: 99%

The effect of water activated by nonthermal air plasma on the growth of farm plants: Case of maize and barley

Yemeli

Švubová

Kostoláni

et al. 2020

Plasma Processes & Polymers

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The effects of plasma‐activated water (PAW) generated by nonthermal air plasmas of transient spark with water electrospray or atmospheric glow discharge were investigated on maize (Zea mays L. var Saccharata) and barley (Hordeum vulgare L.) seedlings. PAW is characterized by measuring concentrations of reactive oxygen and nitrogen species (H2O2, , ). After 4 weeks of plants growth, the effects of PAW are analyzed by measuring plant growth and physiological parameters: plant length and fresh weight, photosynthetic pigments concentration and photosynthesis rate, total soluble proteins, antioxidant enzyme activity, and DNA damage. The results suggest that PAW, depending on chemical composition, has the potential to improve the plant growth and influence the physiological parameters, while causing no harmful DNA damage.

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Entering the plasma agriculture field: An attempt to standardize protocols for plasma treatment of seeds

Waskow

Avino

Howling

et al. 2021

Plasma Processes & Polymers

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Plasma treatments are currently being assessed as a seed processing technology for agricultural purposes. There is sufficient information as a proof-of-concept, but a lack of standardization in the methodology prevents a convincing evaluation of plasma treatment on seeds. It would be helpful to coordinate research efforts to make the entry for newcomers into this interdisciplinary field less overwhelming and to aid in transferring this technology into the industry by establishing a common protocol. This review presents the parameters used in the seed preparation, the plasma treatment of the seed, and the seed posttreatment. This summary of the plasma and biological parameters is intended to raise awareness about questions that need to be addressed to properly record protocol details and reproduce results for the plasma treatment of seeds.

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Influence of Cold Atmospheric Pressure Plasma on Pea Seeds: DNA Damage of Seedlings and Optical Diagnostics of Plasma

Cited by 48 publications

References 44 publications

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

Evaluation of the Impact of Cold Atmospheric Pressure Plasma on Soybean Seed Germination

The effect of water activated by nonthermal air plasma on the growth of farm plants: Case of maize and barley

Entering the plasma agriculture field: An attempt to standardize protocols for plasma treatment of seeds

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