Influence of cold pretreatment on shoot regeneration from callus in date palm (Phoenix dactylifera L.) cv. ‘Barhee’

Abstract

Mass propagation of date palm through indirect somatic embryogenesis or organogenesis has attracted the interest of commercial producers. But, this technique still faces some problems that hindered the production of date palm plantlets in vitro. Tissue browning is one of the serious problems that reduce callus growth and shoot regeneration. So the objective of the present study is to investigate the effect of cold pretreatment on callus growth, shoot regeneration, and polyphenol oxidase (PPO) activity during the callus culture. Results showed that a high survival rate of callus cultures (100%) were obtained when cultures were incubated in low temperature (cold treatment) for 45 and 75 days. On the other hand, total amount on phenolic compounds was also reduced to 0.47 and 0.53 mg GAE/g after same period of incubation (45 and 75 days respectively) at low temperature. In additional, our results showed that the highest frequency of shoot formation (66.67 and 73.34, %) and the highest shoot numbers (7.8 and 8.6 shoots/100 mg) were obtained from callus treated with low temperature for 45 and 75 days, respectively.

Effects of dicamba and casein hydrolysate on in vitro growthand shoot regeneration of date palm (Phoenix dactylifera L.) cv. Barhee

“…Browning is one of the problems facing the practical applications of date palm propagation in vitro, and eliminating or minimizing this phenomenon is essential to establishing a successful culture (Al-Mayahi et al, 2020;Al-Mayahi and Ali, 2021). Phenolic compounds production is correlated with the browning intensity of date palm tissues cultures, and many authors proved these findings in their studies (Al-Mayahi et al, 2018;Shehata et al, 2014), however, Mustafa et al (2013) demonstrated that high concentrations of auxins led to accumulating of phenolic compounds and inhibited the growth of cultures.…”

Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation

“…One of the most significant constraints facing the in vitro tissue of the Date palm is the browning that causes tissue damage a few days after cultivation (Al-Mayahi et al, 2010;Al-Mayahi, 2014). Browning is a significant obstacle to the successful exploitation of these vital trees (Al-Mayahi, 2018 and2019b). Browning of tissues occurs as a result of increased production of TSPCs and their consequent oxidation by the activity of polyphenol oxidase (PPO).…”

Callus Formation and Regeneration Tendency of Justicia vasica Nees under Saline Conditions

“…The browning of explants (Figures 1a and 1b) during tissue culture in culture medium is one of the vital constraints for many plant species, especially for woody species. Usually explant browning is affected by emission of phenolic constituents from the cut ends of explants and its callus restriction mechanism was observed by Al-Mayahi et al [22]. This colorization of explants can be controlled by the addition of phenol absorbents, antioxidants and chelating agents into the medium or transferring the explants into fresh culture media at regular times according to several reports [23,24].…”