Influence of collection and separation of blood samples on plasma IL-1, IL-6 and TNF-α concentrations

Riches, Pamela G.; Gooding, Roger; Millar, Barbara C.; Rowbottom, Anthony W.

doi:10.1016/0022-1759(92)90314-j

Cited by 129 publications

(79 citation statements)

References 7 publications

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“…After exposing mice to SHS from 2 cigarettes for 30 minutes/ day for 4 months, Zhang et al, 2001 noted an increase in interleukin-6, a proinflammatory cytokine. The difference in results between this study and others may be explained by the stability of IL6 which has a shorter half-life (2-6h) than urinary cotinine (Riches et al, 1992) and reflects a more immediate response.…”

Section: Discussioncontrasting

confidence: 90%

The Association Between Environmental Tobacco Smoke and Inflammatory Markers Among Non-Smoker Nurses in Shebin Al-Kom Teaching Hospital

2012

Egypt. J. Occup. Med.

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Abstract:Background: Environmental tobacco smoke (ETS) can cause or exacerbate a wide range of adverse health effects, including cancer, respiratory infections and cardiovascular disease (CVD). There is limited and inconsistent evidence of an association between ETS exposure and inflammatory markers. Aim of the work: To examine the relationship of ETS exposure measured by urinary cotinine level with systemic inflammatory markers that included high-sensitive C reactive protein (hs-CRP), homocysteine, inter-leukin-6 (IL-6), and fibrinogen among non-smoker nurses. Subjects and methods: A cross-sectional study was conducted to study one hundred and forty eight non-smoker nurses at Shebin Al-Kom Teaching hospital. Participants were interviewed and a urine sample for quantitative determination of urinary cotinine level was collected. A blood sample was withdrawn for measuring hs-CRP, homocysteine, IL-6 and fibrinogen levels. Results: With increasing urinary cotinine level, hs-CRP, homocysteine, fibrinogen and IL-6 levels increased, reaching a significant level for hs-CRP and homocysteine not the others. Multivariate regression analysis after adjusting for age, education, BMI and duration of employment, revealed that nurses with ETS exposure had a significant higher levels of hs-CRP, fibrinogen and homocysteine. Summary and recommendations: Regular and repetitive exposure to ETS leads to relevant effects on inflammatory system rather than cytokine system. Longitudinal studies are necessary to determine the potential causal relevance of this

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Section: Discussioncontrasting

confidence: 90%

The Association Between Environmental Tobacco Smoke and Inflammatory Markers Among Non-Smoker Nurses in Shebin Al-Kom Teaching Hospital

2012

Egypt. J. Occup. Med.

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“…TNF-α, measured in serum or plasma is commonly cited as very susceptible to changes in collection and storage methods [13][14][15], and seems to possess the similar vulnerability in mucous samples. The levels of the cytokines TNF-α, IFN-γ and IL-1β, measured in our sample set were well above the detection limits of our assay, and therefore the differences seen were not assay artifacts.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In several cytokine studies, these same factors involving sample collection, processing and storage have been shown to be critical for achieving accurate and reproducible results [2][3][4]. But, most of these published reports on cytokine stability and storage are related to serum or plasma samples [5][6][7][8]. Several studies of cytokines in cervical mucous have been performed to investigate the local immune response to human papillomavirus (HPV), in hopes of determining immune responses associated with clearance or disease progression.…”

Section: Introductionmentioning

confidence: 99%

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Effect of storage temperatures on the stability of cytokines in cervical mucous

Panicker¹,

Meadows²,

Lee³

et al. 2007

Cytokine

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Cytokines have progressively come to serve as indicators for the presence or severity of a disease. But accurate measurement of cytokine levels can be deterred by lack of proper handling and storage of the samples. In this study, we attempted to measure the effect of snap-freezing and refrigeration at the time of collection on cervical mucous. Luminex analyses of the frozen and refrigerated pairs exhibited no significant differences in levels for 7 out of the 10 cytokines measured simultaneously. The cytokines TNF-α, IFN-γ and IL-1β, were significantly different between the pairs with the refrigerated samples showing higher levels for each of these cytokines. The results suggest that refrigeration of mucous samples immediately after collection would allow for better conservation of the cytokines in cervical mucous.

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“…59 Coagulation can activate monocytes during blood sampling. 60 Thawing also increases IL-6 levels, and the type of tubes can affect cytokine measurements. 61 Hence, these factors deserve particular attention.…”

Section: Discussionmentioning

confidence: 99%

The plasma levels of interleukin-12 in schizophrenia, major depression, and bipolar mania: effects of psychotropic drugs

et al. 2002

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Interleukin-12 (IL-12) plays a key role in promoting T helper 1 (Th1) responses and subsequent cell-mediated immunity. Given the role of cytokines in the pathogenesis of psychiatric disorders, the dysregulation of IL-12 in these illnesses would be expected. We measured the plasma levels of IL-12 in 102 psychiatric patients (43 schizophrenia, 34 major depression and 25 bipolar disorder) and 85 normal controls. In addition, IL-12 levels of the patients were measured after an 8-week treatment to assess whether the levels were affected by medication. The IL-12 levels of the patient group with major depression were significantly higher than that of the control group, whereas no differences were found among the other groups. IL-12 values of the three patient groups decreased significantly after 8 weeks of treatment. These findings support the hypothesis that activation of the inflammatory response system and in particular of Th-1-like cells, is involved in the pathophysiology of major depression and that repeated administration of antidepressive and antipsychotic drugs may suppress IL-12 plasma concentrations in psychiatric patients.

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Influence of collection and separation of blood samples on plasma IL-1, IL-6 and TNF-α concentrations

Cited by 129 publications

References 7 publications

The Association Between Environmental Tobacco Smoke and Inflammatory Markers Among Non-Smoker Nurses in Shebin Al-Kom Teaching Hospital

The Association Between Environmental Tobacco Smoke and Inflammatory Markers Among Non-Smoker Nurses in Shebin Al-Kom Teaching Hospital

Effect of storage temperatures on the stability of cytokines in cervical mucous

The plasma levels of interleukin-12 in schizophrenia, major depression, and bipolar mania: effects of psychotropic drugs

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