Influence of colloid fluids on polymorphonuclear granulocyte function <i>in vivo</i>

Engel, J.; Welters, Ingeborg; Rupp, Markus; Langefeld, T. W.; Ruwoldt, Ralph; Menges, T.; Hempelmann, G.

doi:10.1034/j.1399-6576.2001.045003385.x

Cited by 25 publications

(15 citation statements)

References 24 publications

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“…Clinical studies showed blood transfusion to be an independent risk factor for postinjury MOF (10,11). Animal studies demonstrated that different types of resuscitation fluids may affect the inflammatory response and be involved in the pathogenesis of later MOF (9,(14)(15)(16).…”

Section: Discussionmentioning

confidence: 97%

“…Previous studies showed that some artificial colloids have additional immune modulation functions besides volume expansion (14,15). Compared with albumin, hydroxyethyl starch (HES) can reduce splanchnic ischemia and release of circulating adhesion molecules in critically ill patients (16), reduce post-trauma capillary leak, and improve gas exchange (17).…”

Section: Introductionmentioning

confidence: 99%

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Effect of Different Resuscitation Fluids on Cytokine Response in a Rat Model of Hemorrhagic Shock

Lee¹,

Chang

Yen³

et al. 2005

Shock

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This study was designed to determine the effects of different resuscitation fluids on the production of proinflammatory and anti-inflammatory cytokines in an animal model of hemorrhagic shock. Wistar male rats (n = 24; 8/group) were subjected to a volume-controlled hemorrhagic shock for 30 minutes and resuscitated as follows: (1) sham group without resuscitation, (2) lactated Ringer solution (LR), 3:1; (3) 4% hydroxyethyl starch (HES) solution, 1:1; and (4) 4% modified fluid gelatin (GEL), 1:1. Hemodynamic parameters were recorded, and blood samples were collected at 0 min and 30, 90, 150, 210, 270, and 330 min after hemorrhage for plasma levels of IL-6, IL-10, and TNFa. The circulating concentrations of IL-6 at 90, 150, 210, 270, and 330 min and TNFa levels at 150, 210, and 270 min after hemorrhage were significantly elevated in animals resuscitated with GEL compared with HES or LR (P < 0.05). At 210, 270, and 330 min, IL-10 concentration was decreased significantly in GEL-resuscitated rats compared with rats resuscitated with LR or HES (P < 0.05). Mean blood pressure and serum levels of lactate after resuscitation were not different among three kinds of fluids. LR, HES, and GEL are comparable in volume efficacy for resuscitation of hemorrhagic shock but are associated with different postresuscitation immune responses. Resuscitation with GEL may be associated with cytokine production favoring a proinflammatory response. The marked elevation of IL-6 observed in the GEL-treated animals may play a role in the relatively high frequency of anaphylactoid reaction in clinical use of GEL.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Effect of Different Resuscitation Fluids on Cytokine Response in a Rat Model of Hemorrhagic Shock

Lee¹,

Chang

Yen³

et al. 2005

Shock

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“…However, although some authors suggested that differences in the molecular structure of different starch preparations might be responsible for these inconsistent results [34,35], none of the studies to date was able to determine the mechanistic basics that underlie the possibly antiadherent actions of HES. Regarding the well-preserved expression of adhesion molecules on the endothelium [31,32] as well as on the leukocyte [38], effects on protein synthesis or expression do not seem to play a role. Because HES had an immediate effect on PMN adhesion in our experiment only when it was present during perfusion, we suggest that HES affects leukocyte-endothelial interactions by a direct inhibition of ligand-receptor binding.…”

Section: Discussionmentioning

confidence: 99%

Endothelial Accumulation of Hydroxyethyl Starch and Functional Consequences on Leukocyte-Endothelial Interactions

Nohé¹,

Burchard²,

Zanke³

et al. 2002

Eur Surg Res

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To date, accumulation of hydroxyethyl starch (HES) has been studied mainly in skin specimens, but there are no detailed reports available regarding starch accumulation in the endothelium. Because endothelial cells play an essential role during shock, we studied the accumulation of HES in human umbilical venous endothelial cells (HUVEC). HUVEC (n = 9) were incubated with a fluorescein-conjugated HES 200/0.5 (FITC-HES) at 0.5–20 mg/ml for 1–72 h. FITC-HES was internalized dose- and time-dependently by pinocytosis into secondary lysosomes. Asymptotic elimination curves showed that 50% of the formerly ingested molecules could not be eliminated. Despite accumulation, starch molecules did not attenuate the expression of E-selectin, ICAM-1 or VCAM-1 on TNF-α-activated HUVEC. However, apart from adhesion molecule expression, perfusion studies showed that HES reduced neutrophil adhesion by direct inhibition of integrin-mediated interactions.

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“…According to the manufacturer´s instructions, gelatine is hydrolyzed after implantation of the gelatine-coated prosthesis (18). Investigations in plasma-expanding fluid revealed only a marginal PMN receptor upregulation (CD 11b) under the effect provided by gelatine (19). Wilhelm et al (20) verified the strongest antigen-restricted immune response induced by gelatine-coated.…”

Section: Discussionmentioning

confidence: 93%

Effects Of Gelatine-Coated Vascular Grafts On Human Neutrophils

Meyer¹,

Buerger²,

Halloul³

et al. 2015

Polish Journal of Surgery

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6the aim of the study was to investigate the immune-modulatory potential of commercially available PTFE and polyester vascular grafts with and without gelatine-coating. The biomaterial-cell-interaction was characterized by changes of established parameters such as PMN-related receptors/mediators, phagocytosis potential and capacity as well as the effect of an additional plasma-dependent modulation. material and methods. By means of a standardized experimental in vitro model, various vascular graft material (PTFE/polyester/uncoated/gelatine-coated) was used for incubation with or without plasma and co-culturing with human neutrophile granulocytes (PMN) followed by analysis of representative receptors and mediators (CD62L, CD11b, CXCR2, fMLP-R, IL-8, Elastase, LTB 4 ). Oxidative burst assessed phagocytosis capacity. Results. Comparing the vascular grafts, un-coated PTFE induced the lowest magnitude of cell stimulation whereas in case of gelatine-coating, cell response exceeded those of the other vascular grafts. This was also found comparing the polyester-based prosthetic material. Gelatine-coated polyester led to a more pronounced release of elastase than gelatine-coated PTFE and the uncoated materials. The results of oxidative burst indicated a reduced phagocytosis capacity in case of gelatine-coated polyester. Plasma incubation did also provide an impact on the cellular response. While in case of gelatinecoating, PMN-related receptor stimulation became lower, it increased by native polyester. The latter one did also induce more mediators such as IL-8 and LTB 4 than gelatine-coated material. conclusions. There have been no extensive data on cell-cell interactions, cytokines and general histo-/hemocompatibility of human cells by the new generation of vascular grafts. It remains still open whether healing process and infectious resistance can be compromised by material-dependent overstimulation or reduced phagocytosis potential of the immune cells of the primary unspecific immune response induced by gelatine-coated materials.

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Influence of colloid fluids on polymorphonuclear granulocyte function in vivo

Cited by 25 publications

References 24 publications

Effect of Different Resuscitation Fluids on Cytokine Response in a Rat Model of Hemorrhagic Shock

Effect of Different Resuscitation Fluids on Cytokine Response in a Rat Model of Hemorrhagic Shock

Endothelial Accumulation of Hydroxyethyl Starch and Functional Consequences on Leukocyte-Endothelial Interactions

Effects Of Gelatine-Coated Vascular Grafts On Human Neutrophils

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