Influence of cooling temperature in sperm subpopulations of domestic cats

Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples

“…The individual bull factor revealed to be important for every single CASA parameter; this result is linked to the previously demonstrated variability of bull ejaculates among bulls from the same and different breeds [ 25 ], and even from different ejaculates from one single bull [ 12 ]. Due to the lack of homogeneity in breed samples, we cannot conclude that breed would have determined these differences, with this being a limitation of our study.…”

“…In contrast, there is no common methodology to calculate and study sperm subpopulations [ 6 ]. Additionally, it is important to clarify the reasons for the different prevalence of these sperm subpopulations and to assess whether they are of physiological importance [ 12 ].…”

“…CASA values may allow a more accurate prediction of the potential ‘fertility’ of a bull [ 13 ], especially before freezing sperms with fast but nonlinear movements [ 12 ], or even in the case of sperms with a reduced motility [ 11 ]. However, there is controversy about the predictability of fertility from CASA results [ 14 ].…”

“…However, there is controversy about the predictability of fertility from CASA results [ 14 ]. Besides, fertility is variable among bulls, and even between ejaculates from the same bull [ 12 ]. Moreover, the handling of ejaculates seems to be crucial to not interfere in quality assessment, and the conditions of semen storage may affect the sperm kinetics, depending on the extender used, the storage temperature, and the storage duration.…”

Sperm subpopulations influence the pregnancy rates in cattle

“…The computer-assisted sperm analysis (CASA) allows assessing the sperm kinetics and the individual record of their trajectories (Amann & Waberski, 2014;Barbas et al, 2018;Holt & Satake, 2018), based on multivariate statistical analyses to identify and monitor the behaviour of sperm subpopulations within an ejaculate (Souza et al, 2018). The determination of sperm subpopulations based on individual sperm kinetics has been reported in animal species such as domestic cats (Gutiérrez-Reinoso & García-Herreros, 2016;Souza et al, 2018), dogs (Núñez-Martínez et al, 2006;Peña et al, 2012), stallions (Quintero-Moreno et al, 2003), donkeys (Flores et al, 2008), sheep (Barbas et al, 2018;Bergstein-Galan et al, 2017;Dorado et al, 2011;Santolaria et al, 2015), boars (Estrada et al, 2017;Flores et al, 2009), rabbits (Quintero-Moreno et al, 2007) and red deer (Martinez-Pastor et al, 2005). In bulls, studies that evaluated fresh semen and post-thawed samples identified four sperm subpopulations (Ferraz et al, 2014;Ibanescu et al, 2020;Muiño et al, 2009;Muiño, Rivera, et al, 2008;Muiño, Tamargo, et al, 2008).…”