Influence of copper sulfate on anther culture in barley (Hordeum vulgare L.)

Wojnarowiez, Geneviève; Jacquard, Cédric; Devaux, Pierre; Sangwan, Rajbir S.; Clément, Christophe

doi:10.1016/s0168-9452(02)00036-5

Cited by 50 publications

(42 citation statements)

References 16 publications

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“…In cv. Igri, the presence of copper sulphate in the stress treatment medium increases the total number of regenerated plantlets, but has little effect on the production of green plantlets (Wojnarowiez et al 2002). In cv.…”

Section: Discussionmentioning

confidence: 99%

“…Several abiotic stresses, alone or in combination, are used to trigger ME. These include cold shock (Gonzalez and Jouve 2005;Labbani et al 2005), heat-shock (Custers et al 1994;Touraev et al 1996;Rimberia et al 2005), osmotic shock (Caredda et al 2000;Touraev et al 2001;Li and Devaux 2003;Wojnarowiez et al 2004), pH variation (Barinova et al 2004), nutrient starvation (Touraev et al 1996), exposure to heavy metals, such as lithium (Zonia and Tupy 1995) or copper sulphate (Wojnarowiez et al 2002). In barley anthers, ME is induced using a combined coldand osmotic shock treatment (Caredda et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Cork produces exclusively albino plantlets (Caredda and Clément 1999). This problem may be practically overcome by the addition of copper sulphate during anther pre-treatment and culture (Wojnarowiez et al 2002), although the mechanism underlying the beneficial effect of copper is not understood.…”

Section: Introductionmentioning

confidence: 99%

“…Igri and the spring cv. Cork, which are also models for investigating the relationship between ME and albinism in barley (Caredda et al 2000) and (b) in the presence/absence of copper sulphate, owing to its positive effect on ME (Wojnarowiez et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

Jacquard

Mazeyrat-Gourbeyre

Devaux³

et al. 2008

Planta

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Microspore embryogenesis (ME) is a process in which the gametophytic pollen programme of the microspore is reorientated towards a new embryo sporophytic programme. This process requires a stress treatment, usually performed in the anther or isolated microspores for several days. Despite the universal use of stress to induce ME, very few studies have addressed the physiological processes that occur in the anther during this step. To further understand the processes triggered by stress treatment, we followed the response of anthers by measuring the expression of stressrelated genes in two barley (Hordeum vulgare L.) cultivars differing in their ME response. Genes encoding enzymes involved in oxidative stress (glutathione-S-transferase, GST; oxalate oxidase, OxO), in the synthesis of jasmonic acid (13-lipoxygenase, Lox; allene oxide cyclase, AOC; allene oxide synthase, AOS) and in the phenylpropanoid pathway (phenylalanine ammonia lyase, PAL), as well as those encoding PR proteins (Barwin, chitinase 2b, Chit 2b; glucanase, Gluc; basic pathogenesis-related protein 1, PR1; pathogenesis-related protein 10, PR10) were up-regulated in whole anthers upon stress treatment, indicating that anther perceives stress and reacts by triggering general plant defence mechanisms. In particular, both OxO and Chit 2b genes are good markers of anther reactivity owing to their high level of induction during the stress treatment. The effect of copper sulphate appeared to limit the expression of defence-related genes, which may be correlated with its positive effect on the yield of microspore embryos.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

Jacquard

Mazeyrat-Gourbeyre

Devaux³

et al. 2008

Planta

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“…It has been revealed that the number of obtained albino plants depends on the pre-treatment used for androgenesis initiation, the composition of induction medium and in vitro culture conditions (Ć alić et al 2013;Immonen and Robinson 2000;Jacquard et al 2009;Lantos et al 2013;Ś lusarkiewicz-Jarzina and Ponitka 1997;Wojnarowiez et al 2002). Some researches claim that origin of albinism in some cultivars is determined at the earliest phases of ME and obtaining green regenerants depends on the state of microspore plastids at the moment of sampling (Caredda et al 2004(Caredda et al , 2000Muñoz-Amatriaín et al 2009).…”

mentioning

confidence: 99%

Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture

et al. 2015

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High frequency of albino haploids/doubled haploids (DHs), regenerated in androgenic cultures is one of the major obstacles that limit incorporation of DHs technology into cereal breeding programs. Therefore, quantitative trait loci (QTL) associated with albino plant production in triticale anther cultures were analyzed using the population of 90 DH lines derived from F1 cross 'Saka 3006' 9 'Modus'. Composite interval mapping (CIM) and single marker analysis (SMA) in Windows QTL Cartographer ver 2.5 were used to localize the major QTLs. CIM method revealed seven QTLs with LOD scores between 2.9 and 5.6 on five chromosomes from B to R subgenomes (3B, 4B, 4R, 5R and 7R). Effects of all QTLs explained 8.3-17.6 % of the phenotypic variation and were confirmed by SMA analysis. Additionally SMA revealed another seven markers on chromosomes: 2AL.2BL, 3B, 2BS.6AL, 2RS.3R and 4R associated with QTL for albino plant regeneration (p \ 0.01). The additional experiment with ten DH lines varied significantly in their androgenic responsiveness was conducted to analyze the changes in the level of oxidative stress, antioxidative system activity and endogenous hormonal balance associated with androgenesis-inducing low temperature stress treatment (3 weeks at 4°C). The correlation analysis between albino/green plant regeneration ability and analyzed traits were performed by using Spearman Rank test (p B 0.05). Revealed associations may suggest that some level of oxidative stress is necessary for transition from a non-photosynthetic proplastids to the functional chloroplasts. On the other hand, the efficient antioxidative enzyme system and endogenous hormonal balance are also very important. Key message Fourteen chromosome regions were indicated to control albino plant formation during triticale anther culture. Additionally, reactive oxygen species (ROS) generation, antioxidative system activity and hormonal balance were discussed as determinants in androgenesis.

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Haploids in the Improvement of Poaceae

Devaux¹,

Pickering

Haploids in Crop Improvement II

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Influence of copper sulfate on anther culture in barley (Hordeum vulgare L.)

Cited by 50 publications

References 16 publications

Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression

Identification of QTLs associated with albino plant formation and some new facts concerning green versus albino ratio determinants in triticale (×Triticosecale Wittm.) anther culture

Haploids in the Improvement of Poaceae

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