Immature cotton (Gossypium hirsutum L.) fi bers and neps in ginned cotton will aff ect textile quality and thus can aff ect overall industry reputations. Th is study conducted three fi eld studies that systematically varied the timing of harvest aid application to generate diff erences in the amount of immature fi ber and levels of neps in crops at harvest. Th e aim was to ascertain what crop conditions (percent open bolls, number of immature bolls, percent immature bolls, and percent immature lint mass) at the time of application contribute to these diff erences, and assess whether these outcomes are infl uenced by 0, 1, 2 lint cleaning passes. Earlier harvest aid treatments increased neps and the level of neps was best related to fi ber linear density (r 2 = 0.78). All measurements of crop condition at harvest aid application explained changes in yield and fi ber properties well, although the percent immature bolls (r 2 > 0.67) can be applied when crops are nonuniform in their maturity, and when they contain fruiting gaps. Relationships between lint cleaning passes and crop condition at harvest aid application showed an interaction between earlier harvest treatments and lint cleaning passes. One lint cleaning pass contributed between 95 and 141 count g −1 more neps, while a second pass added between 101 and 181 count g −1 more neps. Th is information will be valuable in refi ning strategies that aim to optimize both yield and fi ber quality (including less neps). Th is study also supported the current recommendation of applying harvest aids at 60% open bolls.